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Protein expression and purification of bioactive growth factors for use in cell culture and cellular agriculture
Mitogenic growth factors are major cost drivers in serum-free media, contributing up to 95% of the total cost. Here, we present a streamlined workflow detailing cloning, expression testing, protein purification, and bioactivity screening that allows for low-cost production of bioactive growth factor...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10277608/ https://www.ncbi.nlm.nih.gov/pubmed/37314918 http://dx.doi.org/10.1016/j.xpro.2023.102351 |