Optimized single-cell RNA sequencing protocol to study early genome activation in mammalian preimplantation development

Here, we present a modification of single-cell tagged reverse transcription protocol to study gene expression on a single-cell level or with limited RNA input. We describe different enzymes for reverse transcription and cDNA amplification, modified lysis buffer, and additional clean-up steps before...

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Detalles Bibliográficos
Autores principales: Boskovic, Nina, Yazgeldi, Gamze, Ezer, Sini, Tervaniemi, Mari H., Inzunza, Jose, Deligiannis, Spyridon Panagiotis, Yaşar, Barış, Skoog, Tiina, Krjutškov, Kaarel, Katayama, Shintaro, Kere, Juha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10277609/
https://www.ncbi.nlm.nih.gov/pubmed/37314922
http://dx.doi.org/10.1016/j.xpro.2023.102357

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