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Harnessing the probiotic properties and immunomodulatory effects of fermented food-derived Limosilactobacillus fermentum strains: implications for environmental enteropathy

INTRODUCTION: Environmental enteropathy (EE), a chronic small intestine disease characterized by gut inflammation, is widely prevalent in low-income countries and is hypothesized to be caused by continuous exposure to fecal contamination. Targeted nutritional interventions using potential probiotic...

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Autores principales: Prakash, Vidhya, Madhavan, Aravind, Veedu, Archana Palillam, Babu, Pradeesh, Jothish, Abhirami, Nair, Sruthy S., Suhail, Alin, Prabhakar, Meera, Sain, Thasleema, Rajan, Raveena, Somanathan, Priyanka, Abhinand, Kuniyil, Nair, Bipin G., Pal, Sanjay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10277634/
https://www.ncbi.nlm.nih.gov/pubmed/37342549
http://dx.doi.org/10.3389/fnut.2023.1200926
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author Prakash, Vidhya
Madhavan, Aravind
Veedu, Archana Palillam
Babu, Pradeesh
Jothish, Abhirami
Nair, Sruthy S.
Suhail, Alin
Prabhakar, Meera
Sain, Thasleema
Rajan, Raveena
Somanathan, Priyanka
Abhinand, Kuniyil
Nair, Bipin G.
Pal, Sanjay
author_facet Prakash, Vidhya
Madhavan, Aravind
Veedu, Archana Palillam
Babu, Pradeesh
Jothish, Abhirami
Nair, Sruthy S.
Suhail, Alin
Prabhakar, Meera
Sain, Thasleema
Rajan, Raveena
Somanathan, Priyanka
Abhinand, Kuniyil
Nair, Bipin G.
Pal, Sanjay
author_sort Prakash, Vidhya
collection PubMed
description INTRODUCTION: Environmental enteropathy (EE), a chronic small intestine disease characterized by gut inflammation, is widely prevalent in low-income countries and is hypothesized to be caused by continuous exposure to fecal contamination. Targeted nutritional interventions using potential probiotic strains from fermented foods can be an effective strategy to inhibit enteric pathogens and prevent chronic gut inflammation. METHODS: We isolated potential strains from fermented rice water and lemon pickle and investigated their cell surface properties, antagonistic properties, adhesion to HT-29 cells, and inhibition of pathogen adherence to HT-29 cells. Bacteriocin-like inhibitory substances (BLIS) were purified, and in vivo, survival studies in Caenorhabditis elegans infected with Salmonella enterica MW116733 were performed. We further checked the expression pattern of pro and anti-inflammatory cytokines (IL-6, IL8, and IL-10) in HT-29 cells supplemented with strains. RESULTS: The strains isolated from rice water (RS) and lemon pickle (T1) were identified as Limosilactobacillus fermentum MN410703 and MN410702, respectively. Strains showed probiotic properties like tolerance to low pH (pH 3.0), bile salts up to 0.5%, simulated gastric juice at low pH, and binding to extracellular matrix molecules. Auto-aggregation of T1 was in the range of 85% and significantly co-aggregated with Klebsiella pneumoniae, S. enterica, and Escherichia coli at 48, 79, and 65%, respectively. Both strains had a higher binding affinity to gelatin and heparin compared to Bacillus clausii. Susceptibility to most aminoglycoside, cephalosporin, and macrolide classes of antibiotics was also observed. RS showed BLIS activity against K. pneumoniae, S. aureus, and S. enterica at 60, 48, and 30%, respectively, and the protective effects of BLIS from RS in the C. elegans infection model demonstrated a 70% survival rate of the worms infected with S. enterica. RS and T1 demonstrated binding efficiency to HT-29 cell lines in the 38–46% range, and both strains inhibited the adhesion of E. coli MDR and S. enterica. Upregulation of IL-6 and IL-10 and the downregulation of IL-8 were observed when HT-29 cells were treated with RS, indicating the immunomodulatory effects of the strain. DISCUSSION: The potential strains identified could effectively inhibit enteric pathogens and prevent environmental enteropathy.
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spelling pubmed-102776342023-06-20 Harnessing the probiotic properties and immunomodulatory effects of fermented food-derived Limosilactobacillus fermentum strains: implications for environmental enteropathy Prakash, Vidhya Madhavan, Aravind Veedu, Archana Palillam Babu, Pradeesh Jothish, Abhirami Nair, Sruthy S. Suhail, Alin Prabhakar, Meera Sain, Thasleema Rajan, Raveena Somanathan, Priyanka Abhinand, Kuniyil Nair, Bipin G. Pal, Sanjay Front Nutr Nutrition INTRODUCTION: Environmental enteropathy (EE), a chronic small intestine disease characterized by gut inflammation, is widely prevalent in low-income countries and is hypothesized to be caused by continuous exposure to fecal contamination. Targeted nutritional interventions using potential probiotic strains from fermented foods can be an effective strategy to inhibit enteric pathogens and prevent chronic gut inflammation. METHODS: We isolated potential strains from fermented rice water and lemon pickle and investigated their cell surface properties, antagonistic properties, adhesion to HT-29 cells, and inhibition of pathogen adherence to HT-29 cells. Bacteriocin-like inhibitory substances (BLIS) were purified, and in vivo, survival studies in Caenorhabditis elegans infected with Salmonella enterica MW116733 were performed. We further checked the expression pattern of pro and anti-inflammatory cytokines (IL-6, IL8, and IL-10) in HT-29 cells supplemented with strains. RESULTS: The strains isolated from rice water (RS) and lemon pickle (T1) were identified as Limosilactobacillus fermentum MN410703 and MN410702, respectively. Strains showed probiotic properties like tolerance to low pH (pH 3.0), bile salts up to 0.5%, simulated gastric juice at low pH, and binding to extracellular matrix molecules. Auto-aggregation of T1 was in the range of 85% and significantly co-aggregated with Klebsiella pneumoniae, S. enterica, and Escherichia coli at 48, 79, and 65%, respectively. Both strains had a higher binding affinity to gelatin and heparin compared to Bacillus clausii. Susceptibility to most aminoglycoside, cephalosporin, and macrolide classes of antibiotics was also observed. RS showed BLIS activity against K. pneumoniae, S. aureus, and S. enterica at 60, 48, and 30%, respectively, and the protective effects of BLIS from RS in the C. elegans infection model demonstrated a 70% survival rate of the worms infected with S. enterica. RS and T1 demonstrated binding efficiency to HT-29 cell lines in the 38–46% range, and both strains inhibited the adhesion of E. coli MDR and S. enterica. Upregulation of IL-6 and IL-10 and the downregulation of IL-8 were observed when HT-29 cells were treated with RS, indicating the immunomodulatory effects of the strain. DISCUSSION: The potential strains identified could effectively inhibit enteric pathogens and prevent environmental enteropathy. Frontiers Media S.A. 2023-06-05 /pmc/articles/PMC10277634/ /pubmed/37342549 http://dx.doi.org/10.3389/fnut.2023.1200926 Text en Copyright © 2023 Prakash, Madhavan, Veedu, Babu, Jothish, Nair, Suhail, Prabhakar, Sain, Rajan, Somanathan, Abhinand, Nair and Pal. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Prakash, Vidhya
Madhavan, Aravind
Veedu, Archana Palillam
Babu, Pradeesh
Jothish, Abhirami
Nair, Sruthy S.
Suhail, Alin
Prabhakar, Meera
Sain, Thasleema
Rajan, Raveena
Somanathan, Priyanka
Abhinand, Kuniyil
Nair, Bipin G.
Pal, Sanjay
Harnessing the probiotic properties and immunomodulatory effects of fermented food-derived Limosilactobacillus fermentum strains: implications for environmental enteropathy
title Harnessing the probiotic properties and immunomodulatory effects of fermented food-derived Limosilactobacillus fermentum strains: implications for environmental enteropathy
title_full Harnessing the probiotic properties and immunomodulatory effects of fermented food-derived Limosilactobacillus fermentum strains: implications for environmental enteropathy
title_fullStr Harnessing the probiotic properties and immunomodulatory effects of fermented food-derived Limosilactobacillus fermentum strains: implications for environmental enteropathy
title_full_unstemmed Harnessing the probiotic properties and immunomodulatory effects of fermented food-derived Limosilactobacillus fermentum strains: implications for environmental enteropathy
title_short Harnessing the probiotic properties and immunomodulatory effects of fermented food-derived Limosilactobacillus fermentum strains: implications for environmental enteropathy
title_sort harnessing the probiotic properties and immunomodulatory effects of fermented food-derived limosilactobacillus fermentum strains: implications for environmental enteropathy
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10277634/
https://www.ncbi.nlm.nih.gov/pubmed/37342549
http://dx.doi.org/10.3389/fnut.2023.1200926
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