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Identification of differentially expressed HERV-K(HML-2) loci in colorectal cancer
Colorectal cancer is one of the malignant tumors with the highest mortality rate in the world. Survival rates vary significantly among patients at various stages of the disease. A biomarker capable of early diagnosis is required to facilitate the early detection and treatment of colorectal cancer. H...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10277637/ https://www.ncbi.nlm.nih.gov/pubmed/37342563 http://dx.doi.org/10.3389/fmicb.2023.1192900 |
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author | Kang, Qian Guo, Xin Li, Tianfu Yang, Caiqin Han, Jingwan Jia, Lei Liu, Yongjian Wang, Xiaolin Zhang, Bohan Li, Jingyun Wen, Hong-Ling Li, Hanping Li, Lin |
author_facet | Kang, Qian Guo, Xin Li, Tianfu Yang, Caiqin Han, Jingwan Jia, Lei Liu, Yongjian Wang, Xiaolin Zhang, Bohan Li, Jingyun Wen, Hong-Ling Li, Hanping Li, Lin |
author_sort | Kang, Qian |
collection | PubMed |
description | Colorectal cancer is one of the malignant tumors with the highest mortality rate in the world. Survival rates vary significantly among patients at various stages of the disease. A biomarker capable of early diagnosis is required to facilitate the early detection and treatment of colorectal cancer. Human endogenous retroviruses (HERVs) are abnormally expressed in various diseases, including cancer, and have been involved in cancer development. Real-time quantitative PCR was used to detect the transcript levels of HERV-K(HML-2) gag, pol, and env in colorectal cancer to systematically investigate the connection between HERV-K(HML-2) and colorectal cancer. The results showed that HERV-K(HML-2) transcript expression was significantly higher than healthy controls and was consistent at the population and cell levels. We also used next-generation sequencing to identify and characterize HERV-K(HML-2) loci that were differentially expressed between colorectal cancer patients and healthy individuals. The analysis revealed that these loci were concentrated in immune response signaling pathways, implying that HERV-K impacts the tumor-associated immune response. Our results indicated that HERV-K might serve as a screening tumor marker and a target for tumor immunotherapy in colorectal cancer. |
format | Online Article Text |
id | pubmed-10277637 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102776372023-06-20 Identification of differentially expressed HERV-K(HML-2) loci in colorectal cancer Kang, Qian Guo, Xin Li, Tianfu Yang, Caiqin Han, Jingwan Jia, Lei Liu, Yongjian Wang, Xiaolin Zhang, Bohan Li, Jingyun Wen, Hong-Ling Li, Hanping Li, Lin Front Microbiol Microbiology Colorectal cancer is one of the malignant tumors with the highest mortality rate in the world. Survival rates vary significantly among patients at various stages of the disease. A biomarker capable of early diagnosis is required to facilitate the early detection and treatment of colorectal cancer. Human endogenous retroviruses (HERVs) are abnormally expressed in various diseases, including cancer, and have been involved in cancer development. Real-time quantitative PCR was used to detect the transcript levels of HERV-K(HML-2) gag, pol, and env in colorectal cancer to systematically investigate the connection between HERV-K(HML-2) and colorectal cancer. The results showed that HERV-K(HML-2) transcript expression was significantly higher than healthy controls and was consistent at the population and cell levels. We also used next-generation sequencing to identify and characterize HERV-K(HML-2) loci that were differentially expressed between colorectal cancer patients and healthy individuals. The analysis revealed that these loci were concentrated in immune response signaling pathways, implying that HERV-K impacts the tumor-associated immune response. Our results indicated that HERV-K might serve as a screening tumor marker and a target for tumor immunotherapy in colorectal cancer. Frontiers Media S.A. 2023-06-05 /pmc/articles/PMC10277637/ /pubmed/37342563 http://dx.doi.org/10.3389/fmicb.2023.1192900 Text en Copyright © 2023 Kang, Guo, Li, Yang, Han, Jia, Liu, Wang, Zhang, Li, Wen, Li and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Kang, Qian Guo, Xin Li, Tianfu Yang, Caiqin Han, Jingwan Jia, Lei Liu, Yongjian Wang, Xiaolin Zhang, Bohan Li, Jingyun Wen, Hong-Ling Li, Hanping Li, Lin Identification of differentially expressed HERV-K(HML-2) loci in colorectal cancer |
title | Identification of differentially expressed HERV-K(HML-2) loci in colorectal cancer |
title_full | Identification of differentially expressed HERV-K(HML-2) loci in colorectal cancer |
title_fullStr | Identification of differentially expressed HERV-K(HML-2) loci in colorectal cancer |
title_full_unstemmed | Identification of differentially expressed HERV-K(HML-2) loci in colorectal cancer |
title_short | Identification of differentially expressed HERV-K(HML-2) loci in colorectal cancer |
title_sort | identification of differentially expressed herv-k(hml-2) loci in colorectal cancer |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10277637/ https://www.ncbi.nlm.nih.gov/pubmed/37342563 http://dx.doi.org/10.3389/fmicb.2023.1192900 |
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