Cargando…
Cellular response of Parachlorella kessleri to a solid surface culture environment
Attached culture allows high biomass productivity and is a promising biomass cultivating system because neither a huge facility area nor a large volume of culture medium are needed. This study investigates photosynthetic and transcriptomic behaviors in Parachlorella kessleri cells on a solid surface...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10277731/ https://www.ncbi.nlm.nih.gov/pubmed/37342150 http://dx.doi.org/10.3389/fpls.2023.1175080 |
_version_ | 1785060349561536512 |
---|---|
author | Miyauchi, Hiroki Ishikawa, Tomoharu Hirakawa, Yutaro Sudou, Ayumu Okada, Katsuhiko Hijikata, Atsushi Sato, Norihiro Tsuzuki, Mikio Fujiwara, Shoko |
author_facet | Miyauchi, Hiroki Ishikawa, Tomoharu Hirakawa, Yutaro Sudou, Ayumu Okada, Katsuhiko Hijikata, Atsushi Sato, Norihiro Tsuzuki, Mikio Fujiwara, Shoko |
author_sort | Miyauchi, Hiroki |
collection | PubMed |
description | Attached culture allows high biomass productivity and is a promising biomass cultivating system because neither a huge facility area nor a large volume of culture medium are needed. This study investigates photosynthetic and transcriptomic behaviors in Parachlorella kessleri cells on a solid surface after their transfer from liquid culture to elucidate the physiological and gene-expression regulatory mechanisms that underlie their vigorous proliferation. The chlorophyll content shows a decrease at 12 h after the transfer; however, it has fully recovered at 24 h, suggesting temporary decreases in the amounts of light harvesting complexes. On PAM analysis, it is demonstrated that the effective quantum yield of PSII decreases at 0 h right after the transfer, followed by its recovery in the next 24 h. A similar changing pattern is observed for the photochemical quenching, with the PSII maximum quantum yield remaining at an almost unaltered level. Non-photochemical quenching was increased at both 0 h and 12 h after the transfer. These observations suggest that electron transfer downstream of PSII but not PSII itself is only temporarily damaged in solid-surface cells just after the transfer, with light energy in excess being dissipated as heat for PSII protection. It thus seems that the photosynthetic machinery acclimates to high-light and/or dehydration stresses through its temporal size-down and functional regulation that start right after the transfer. Meanwhile, transcriptomic analysis by RNA-Seq demonstrates temporary upregulation at 12 h after the transfer as to the expression levels of many genes for photosynthesis, amino acid synthesis, general stress response, and ribosomal subunit proteins. These findings suggest that cells transferred to a solid surface become stressed immediately after transfer but can recover their high photosynthetic activity through adaptation of photosynthetic machinery and metabolic flow as well as induction of general stress response mechanisms within 24 h. |
format | Online Article Text |
id | pubmed-10277731 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102777312023-06-20 Cellular response of Parachlorella kessleri to a solid surface culture environment Miyauchi, Hiroki Ishikawa, Tomoharu Hirakawa, Yutaro Sudou, Ayumu Okada, Katsuhiko Hijikata, Atsushi Sato, Norihiro Tsuzuki, Mikio Fujiwara, Shoko Front Plant Sci Plant Science Attached culture allows high biomass productivity and is a promising biomass cultivating system because neither a huge facility area nor a large volume of culture medium are needed. This study investigates photosynthetic and transcriptomic behaviors in Parachlorella kessleri cells on a solid surface after their transfer from liquid culture to elucidate the physiological and gene-expression regulatory mechanisms that underlie their vigorous proliferation. The chlorophyll content shows a decrease at 12 h after the transfer; however, it has fully recovered at 24 h, suggesting temporary decreases in the amounts of light harvesting complexes. On PAM analysis, it is demonstrated that the effective quantum yield of PSII decreases at 0 h right after the transfer, followed by its recovery in the next 24 h. A similar changing pattern is observed for the photochemical quenching, with the PSII maximum quantum yield remaining at an almost unaltered level. Non-photochemical quenching was increased at both 0 h and 12 h after the transfer. These observations suggest that electron transfer downstream of PSII but not PSII itself is only temporarily damaged in solid-surface cells just after the transfer, with light energy in excess being dissipated as heat for PSII protection. It thus seems that the photosynthetic machinery acclimates to high-light and/or dehydration stresses through its temporal size-down and functional regulation that start right after the transfer. Meanwhile, transcriptomic analysis by RNA-Seq demonstrates temporary upregulation at 12 h after the transfer as to the expression levels of many genes for photosynthesis, amino acid synthesis, general stress response, and ribosomal subunit proteins. These findings suggest that cells transferred to a solid surface become stressed immediately after transfer but can recover their high photosynthetic activity through adaptation of photosynthetic machinery and metabolic flow as well as induction of general stress response mechanisms within 24 h. Frontiers Media S.A. 2023-06-05 /pmc/articles/PMC10277731/ /pubmed/37342150 http://dx.doi.org/10.3389/fpls.2023.1175080 Text en Copyright © 2023 Miyauchi, Ishikawa, Hirakawa, Sudou, Okada, Hijikata, Sato, Tsuzuki and Fujiwara https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Miyauchi, Hiroki Ishikawa, Tomoharu Hirakawa, Yutaro Sudou, Ayumu Okada, Katsuhiko Hijikata, Atsushi Sato, Norihiro Tsuzuki, Mikio Fujiwara, Shoko Cellular response of Parachlorella kessleri to a solid surface culture environment |
title | Cellular response of Parachlorella kessleri to a solid surface culture environment |
title_full | Cellular response of Parachlorella kessleri to a solid surface culture environment |
title_fullStr | Cellular response of Parachlorella kessleri to a solid surface culture environment |
title_full_unstemmed | Cellular response of Parachlorella kessleri to a solid surface culture environment |
title_short | Cellular response of Parachlorella kessleri to a solid surface culture environment |
title_sort | cellular response of parachlorella kessleri to a solid surface culture environment |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10277731/ https://www.ncbi.nlm.nih.gov/pubmed/37342150 http://dx.doi.org/10.3389/fpls.2023.1175080 |
work_keys_str_mv | AT miyauchihiroki cellularresponseofparachlorellakessleritoasolidsurfacecultureenvironment AT ishikawatomoharu cellularresponseofparachlorellakessleritoasolidsurfacecultureenvironment AT hirakawayutaro cellularresponseofparachlorellakessleritoasolidsurfacecultureenvironment AT sudouayumu cellularresponseofparachlorellakessleritoasolidsurfacecultureenvironment AT okadakatsuhiko cellularresponseofparachlorellakessleritoasolidsurfacecultureenvironment AT hijikataatsushi cellularresponseofparachlorellakessleritoasolidsurfacecultureenvironment AT satonorihiro cellularresponseofparachlorellakessleritoasolidsurfacecultureenvironment AT tsuzukimikio cellularresponseofparachlorellakessleritoasolidsurfacecultureenvironment AT fujiwarashoko cellularresponseofparachlorellakessleritoasolidsurfacecultureenvironment |