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Transcriptome analysis reveals the high ribosomal inhibitory action of 1,4-naphthoquinone on Meloidogyne luci infective second-stage juveniles

The root-knot nematode (RKN) Meloidogyne luci presents a threat to the production of several important crops. This nematode species was added to the European Plant Protection Organization Alert list in 2017. The scarce availability of efficient nematicides to control RKN and the phasing out of nemat...

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Autores principales: Cardoso, Joana M. S., Esteves, Ivânia, Egas, Conceição, Braga, Mara E. M., de Sousa, Hermínio C., Abrantes, Isabel, Maleita, Carla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10277735/
https://www.ncbi.nlm.nih.gov/pubmed/37342130
http://dx.doi.org/10.3389/fpls.2023.1191923
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author Cardoso, Joana M. S.
Esteves, Ivânia
Egas, Conceição
Braga, Mara E. M.
de Sousa, Hermínio C.
Abrantes, Isabel
Maleita, Carla
author_facet Cardoso, Joana M. S.
Esteves, Ivânia
Egas, Conceição
Braga, Mara E. M.
de Sousa, Hermínio C.
Abrantes, Isabel
Maleita, Carla
author_sort Cardoso, Joana M. S.
collection PubMed
description The root-knot nematode (RKN) Meloidogyne luci presents a threat to the production of several important crops. This nematode species was added to the European Plant Protection Organization Alert list in 2017. The scarce availability of efficient nematicides to control RKN and the phasing out of nematicides from the market have intensified the search for alternatives, such as phytochemicals with bionematicidal properties. The nematicidal activity of 1,4-naphthoquinone (1,4-NTQ) against M. luci has been demonstrated; however, knowledge of the potential mode(s) of action of this compound is still scarce. In this study, the transcriptome profile of M. luci second-stage juveniles (J2), the infective stage, in response to 1,4-NTQ exposure was determined by RNA-seq to identify genes and pathways that might be involved in 1,4-NTQ’s mode(s) of action. Control treatments, consisting of nematodes exposed to Tween(®) 80 (1,4-NTQ solvent) and to water, were included in the analysis. A large set of differentially expressed genes (DEGs) was found among the three tested conditions, and a high number of downregulated genes were found between 1,4-NTQ treatment and water control, reflecting the inhibitory effect of this compound on M. luci, with a great impact on processes related to translation (ribosome pathway). Several other nematode gene networks and metabolic pathways affected by 1,4-NTQ were also identified, clarifying the possible mode of action of this promising bionematicide.
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spelling pubmed-102777352023-06-20 Transcriptome analysis reveals the high ribosomal inhibitory action of 1,4-naphthoquinone on Meloidogyne luci infective second-stage juveniles Cardoso, Joana M. S. Esteves, Ivânia Egas, Conceição Braga, Mara E. M. de Sousa, Hermínio C. Abrantes, Isabel Maleita, Carla Front Plant Sci Plant Science The root-knot nematode (RKN) Meloidogyne luci presents a threat to the production of several important crops. This nematode species was added to the European Plant Protection Organization Alert list in 2017. The scarce availability of efficient nematicides to control RKN and the phasing out of nematicides from the market have intensified the search for alternatives, such as phytochemicals with bionematicidal properties. The nematicidal activity of 1,4-naphthoquinone (1,4-NTQ) against M. luci has been demonstrated; however, knowledge of the potential mode(s) of action of this compound is still scarce. In this study, the transcriptome profile of M. luci second-stage juveniles (J2), the infective stage, in response to 1,4-NTQ exposure was determined by RNA-seq to identify genes and pathways that might be involved in 1,4-NTQ’s mode(s) of action. Control treatments, consisting of nematodes exposed to Tween(®) 80 (1,4-NTQ solvent) and to water, were included in the analysis. A large set of differentially expressed genes (DEGs) was found among the three tested conditions, and a high number of downregulated genes were found between 1,4-NTQ treatment and water control, reflecting the inhibitory effect of this compound on M. luci, with a great impact on processes related to translation (ribosome pathway). Several other nematode gene networks and metabolic pathways affected by 1,4-NTQ were also identified, clarifying the possible mode of action of this promising bionematicide. Frontiers Media S.A. 2023-06-05 /pmc/articles/PMC10277735/ /pubmed/37342130 http://dx.doi.org/10.3389/fpls.2023.1191923 Text en Copyright © 2023 Cardoso, Esteves, Egas, Braga, de Sousa, Abrantes and Maleita https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Cardoso, Joana M. S.
Esteves, Ivânia
Egas, Conceição
Braga, Mara E. M.
de Sousa, Hermínio C.
Abrantes, Isabel
Maleita, Carla
Transcriptome analysis reveals the high ribosomal inhibitory action of 1,4-naphthoquinone on Meloidogyne luci infective second-stage juveniles
title Transcriptome analysis reveals the high ribosomal inhibitory action of 1,4-naphthoquinone on Meloidogyne luci infective second-stage juveniles
title_full Transcriptome analysis reveals the high ribosomal inhibitory action of 1,4-naphthoquinone on Meloidogyne luci infective second-stage juveniles
title_fullStr Transcriptome analysis reveals the high ribosomal inhibitory action of 1,4-naphthoquinone on Meloidogyne luci infective second-stage juveniles
title_full_unstemmed Transcriptome analysis reveals the high ribosomal inhibitory action of 1,4-naphthoquinone on Meloidogyne luci infective second-stage juveniles
title_short Transcriptome analysis reveals the high ribosomal inhibitory action of 1,4-naphthoquinone on Meloidogyne luci infective second-stage juveniles
title_sort transcriptome analysis reveals the high ribosomal inhibitory action of 1,4-naphthoquinone on meloidogyne luci infective second-stage juveniles
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10277735/
https://www.ncbi.nlm.nih.gov/pubmed/37342130
http://dx.doi.org/10.3389/fpls.2023.1191923
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