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Genomic characterization of the rotavirus G3P[8] strain in vaccinated children, reveals possible reassortment events between human and animal strains in Manhiça District, Mozambique

Mozambique introduced the rotavirus vaccine (Rotarix®; GlaxoSmithKline Biologicals, Rixensart, Belgium) in 2015, and since then, the Centro de Investigação em Saúde de Manhiça has been monitoring its impact on rotavirus-associated diarrhea and the trend of circulating strains, where G3P[8] was repor...

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Autores principales: Manjate, Filomena, João, Eva D., Mwangi, Peter, Chirinda, Percina, Mogotsi, Milton, Messa, Augusto, Garrine, Marcelino, Vubil, Delfino, Nobela, Nélio, Nhampossa, Tacilta, Acácio, Sozinho, Tate, Jacqueline E., Parashar, Umesh, Weldegebriel, Goitom, Mwenda, Jason M., Alonso, Pedro L., Cunha, Celso, Nyaga, Martin, Mandomando, Inácio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10277737/
https://www.ncbi.nlm.nih.gov/pubmed/37342557
http://dx.doi.org/10.3389/fmicb.2023.1193094
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author Manjate, Filomena
João, Eva D.
Mwangi, Peter
Chirinda, Percina
Mogotsi, Milton
Messa, Augusto
Garrine, Marcelino
Vubil, Delfino
Nobela, Nélio
Nhampossa, Tacilta
Acácio, Sozinho
Tate, Jacqueline E.
Parashar, Umesh
Weldegebriel, Goitom
Mwenda, Jason M.
Alonso, Pedro L.
Cunha, Celso
Nyaga, Martin
Mandomando, Inácio
author_facet Manjate, Filomena
João, Eva D.
Mwangi, Peter
Chirinda, Percina
Mogotsi, Milton
Messa, Augusto
Garrine, Marcelino
Vubil, Delfino
Nobela, Nélio
Nhampossa, Tacilta
Acácio, Sozinho
Tate, Jacqueline E.
Parashar, Umesh
Weldegebriel, Goitom
Mwenda, Jason M.
Alonso, Pedro L.
Cunha, Celso
Nyaga, Martin
Mandomando, Inácio
author_sort Manjate, Filomena
collection PubMed
description Mozambique introduced the rotavirus vaccine (Rotarix®; GlaxoSmithKline Biologicals, Rixensart, Belgium) in 2015, and since then, the Centro de Investigação em Saúde de Manhiça has been monitoring its impact on rotavirus-associated diarrhea and the trend of circulating strains, where G3P[8] was reported as the predominant strain after the vaccine introduction. Genotype G3 is among the most commonly detected Rotavirus strains in humans and animals, and herein, we report on the whole genome constellation of G3P[8] detected in two children (aged 18 months old) hospitalized with moderate-to-severe diarrhea at the Manhiça District Hospital. The two strains had a typical Wa-like genome constellation (I1-R1-C1-M1-A1-N1-T1-E1-H1) and shared 100% nucleotide (nt) and amino acid (aa) identities in 10 gene segments, except for VP6. Phylogenetic analysis demonstrated that genome segments encoding VP7, VP6, VP1, NSP3, and NSP4 of the two strains clustered most closely with porcine, bovine, and equine strains with identities ranging from 86.9–99.9% nt and 97.2–100% aa. Moreover, they consistently formed distinct clusters with some G1P[8], G3P[8], G9P[8], G12P[6], and G12P[8] strains circulating from 2012 to 2019 in Africa (Mozambique, Kenya, Rwanda, and Malawi) and Asia (Japan, China, and India) in genome segments encoding six proteins (VP2, VP3, NSP1-NSP2, NSP5/6). The identification of segments exhibiting the closest relationships with animal strains shows significant diversity of rotavirus and suggests the possible occurrence of reassortment events between human and animal strains. This demonstrates the importance of applying next-generation sequencing to monitor and understand the evolutionary changes of strains and evaluate the impact of vaccines on strain diversity.
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spelling pubmed-102777372023-06-20 Genomic characterization of the rotavirus G3P[8] strain in vaccinated children, reveals possible reassortment events between human and animal strains in Manhiça District, Mozambique Manjate, Filomena João, Eva D. Mwangi, Peter Chirinda, Percina Mogotsi, Milton Messa, Augusto Garrine, Marcelino Vubil, Delfino Nobela, Nélio Nhampossa, Tacilta Acácio, Sozinho Tate, Jacqueline E. Parashar, Umesh Weldegebriel, Goitom Mwenda, Jason M. Alonso, Pedro L. Cunha, Celso Nyaga, Martin Mandomando, Inácio Front Microbiol Microbiology Mozambique introduced the rotavirus vaccine (Rotarix®; GlaxoSmithKline Biologicals, Rixensart, Belgium) in 2015, and since then, the Centro de Investigação em Saúde de Manhiça has been monitoring its impact on rotavirus-associated diarrhea and the trend of circulating strains, where G3P[8] was reported as the predominant strain after the vaccine introduction. Genotype G3 is among the most commonly detected Rotavirus strains in humans and animals, and herein, we report on the whole genome constellation of G3P[8] detected in two children (aged 18 months old) hospitalized with moderate-to-severe diarrhea at the Manhiça District Hospital. The two strains had a typical Wa-like genome constellation (I1-R1-C1-M1-A1-N1-T1-E1-H1) and shared 100% nucleotide (nt) and amino acid (aa) identities in 10 gene segments, except for VP6. Phylogenetic analysis demonstrated that genome segments encoding VP7, VP6, VP1, NSP3, and NSP4 of the two strains clustered most closely with porcine, bovine, and equine strains with identities ranging from 86.9–99.9% nt and 97.2–100% aa. Moreover, they consistently formed distinct clusters with some G1P[8], G3P[8], G9P[8], G12P[6], and G12P[8] strains circulating from 2012 to 2019 in Africa (Mozambique, Kenya, Rwanda, and Malawi) and Asia (Japan, China, and India) in genome segments encoding six proteins (VP2, VP3, NSP1-NSP2, NSP5/6). The identification of segments exhibiting the closest relationships with animal strains shows significant diversity of rotavirus and suggests the possible occurrence of reassortment events between human and animal strains. This demonstrates the importance of applying next-generation sequencing to monitor and understand the evolutionary changes of strains and evaluate the impact of vaccines on strain diversity. Frontiers Media S.A. 2023-06-05 /pmc/articles/PMC10277737/ /pubmed/37342557 http://dx.doi.org/10.3389/fmicb.2023.1193094 Text en Copyright © 2023 Manjate, João, Mwangi, Chirinda, Mogotsi, Messa, Garrine, Vubil, Nobela, Nhampossa, Acácio, Tate, Parashar, Weldegebriel, Mwenda, Alonso, Cunha, Nyaga and Mandomando. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Manjate, Filomena
João, Eva D.
Mwangi, Peter
Chirinda, Percina
Mogotsi, Milton
Messa, Augusto
Garrine, Marcelino
Vubil, Delfino
Nobela, Nélio
Nhampossa, Tacilta
Acácio, Sozinho
Tate, Jacqueline E.
Parashar, Umesh
Weldegebriel, Goitom
Mwenda, Jason M.
Alonso, Pedro L.
Cunha, Celso
Nyaga, Martin
Mandomando, Inácio
Genomic characterization of the rotavirus G3P[8] strain in vaccinated children, reveals possible reassortment events between human and animal strains in Manhiça District, Mozambique
title Genomic characterization of the rotavirus G3P[8] strain in vaccinated children, reveals possible reassortment events between human and animal strains in Manhiça District, Mozambique
title_full Genomic characterization of the rotavirus G3P[8] strain in vaccinated children, reveals possible reassortment events between human and animal strains in Manhiça District, Mozambique
title_fullStr Genomic characterization of the rotavirus G3P[8] strain in vaccinated children, reveals possible reassortment events between human and animal strains in Manhiça District, Mozambique
title_full_unstemmed Genomic characterization of the rotavirus G3P[8] strain in vaccinated children, reveals possible reassortment events between human and animal strains in Manhiça District, Mozambique
title_short Genomic characterization of the rotavirus G3P[8] strain in vaccinated children, reveals possible reassortment events between human and animal strains in Manhiça District, Mozambique
title_sort genomic characterization of the rotavirus g3p[8] strain in vaccinated children, reveals possible reassortment events between human and animal strains in manhiça district, mozambique
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10277737/
https://www.ncbi.nlm.nih.gov/pubmed/37342557
http://dx.doi.org/10.3389/fmicb.2023.1193094
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