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The Effect of miR-4800 Restoration on Proliferation and Migration of Human Breast Cancer Cells In Vitro
Purpose: MicroRNAs (miRNAs) can contribute to cancer initiation, development, and progression. In this study, the effect of miRNA-4800 restoration on the growth and migration inhibition of human breast cancer (BC) cells was investigated. Methods: For this purpose, transfection of miR-4800 was perfor...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tabriz University of Medical Sciences
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10278211/ https://www.ncbi.nlm.nih.gov/pubmed/37342379 http://dx.doi.org/10.34172/apb.2023.041 |
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author | Khordadmehr, Monireh Matin, Reyhaneh Baradaran, Behzad Baghbani, Elham Jigari-Asl, Farinaz Noorolyai, Saeed |
author_facet | Khordadmehr, Monireh Matin, Reyhaneh Baradaran, Behzad Baghbani, Elham Jigari-Asl, Farinaz Noorolyai, Saeed |
author_sort | Khordadmehr, Monireh |
collection | PubMed |
description | Purpose: MicroRNAs (miRNAs) can contribute to cancer initiation, development, and progression. In this study, the effect of miRNA-4800 restoration on the growth and migration inhibition of human breast cancer (BC) cells was investigated. Methods: For this purpose, transfection of miR-4800 was performed into MDA-MB-231 BC cells using jetPEI. Subsequently, the expression levels of miR-4800 and CXCR4, ROCK1, CD44, and vimentin genes were measured using quantitative real-time polymerase chain reaction (q-RT-PCR) and specific primers. Also, the proliferation inhibition and apoptosis induction of cancer cells were evaluated by MTT and flow cytometry (Annexin V-PI method) techniques, respectively. Additionally, cancer cell migration after miR-4800 transfection was assessed by wound-healing (scratch) assay. Results: The restoration of miR-4800 in MDA-MB-231 cells resulted in the decreased expression level of CXCR4 (P ˂ 0.01), ROCK1 (P ˂ 0.0001), CD44 (P ˂ 0.0001), and vimentin (P ˂ 0.0001) genes. Also, MTT results showed restoration of miR-4800 could significantly reduce cell viability rate (P ˂ 0.0001) compared with the control group. Cell migration remarkably inhibited (P ˂ 0.001) upon miR-4800 transfection in treated BC cells. Flow cytometry data demonstrated that miR-4800 replacement considerably induced apoptosis in cancer cells (P ˂ 0.001) compared with control cells. Conclusion: Taken together, it seems that miR-4800 can act as a tumor suppressor miRNA in BC and play an essential role in modulating apoptosis, migration, and metastasis in BC. Therefore, it may be suggested as a potential therapeutic target in treating BC by performing additional tests in the future. |
format | Online Article Text |
id | pubmed-10278211 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Tabriz University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-102782112023-06-20 The Effect of miR-4800 Restoration on Proliferation and Migration of Human Breast Cancer Cells In Vitro Khordadmehr, Monireh Matin, Reyhaneh Baradaran, Behzad Baghbani, Elham Jigari-Asl, Farinaz Noorolyai, Saeed Adv Pharm Bull Research Article Purpose: MicroRNAs (miRNAs) can contribute to cancer initiation, development, and progression. In this study, the effect of miRNA-4800 restoration on the growth and migration inhibition of human breast cancer (BC) cells was investigated. Methods: For this purpose, transfection of miR-4800 was performed into MDA-MB-231 BC cells using jetPEI. Subsequently, the expression levels of miR-4800 and CXCR4, ROCK1, CD44, and vimentin genes were measured using quantitative real-time polymerase chain reaction (q-RT-PCR) and specific primers. Also, the proliferation inhibition and apoptosis induction of cancer cells were evaluated by MTT and flow cytometry (Annexin V-PI method) techniques, respectively. Additionally, cancer cell migration after miR-4800 transfection was assessed by wound-healing (scratch) assay. Results: The restoration of miR-4800 in MDA-MB-231 cells resulted in the decreased expression level of CXCR4 (P ˂ 0.01), ROCK1 (P ˂ 0.0001), CD44 (P ˂ 0.0001), and vimentin (P ˂ 0.0001) genes. Also, MTT results showed restoration of miR-4800 could significantly reduce cell viability rate (P ˂ 0.0001) compared with the control group. Cell migration remarkably inhibited (P ˂ 0.001) upon miR-4800 transfection in treated BC cells. Flow cytometry data demonstrated that miR-4800 replacement considerably induced apoptosis in cancer cells (P ˂ 0.001) compared with control cells. Conclusion: Taken together, it seems that miR-4800 can act as a tumor suppressor miRNA in BC and play an essential role in modulating apoptosis, migration, and metastasis in BC. Therefore, it may be suggested as a potential therapeutic target in treating BC by performing additional tests in the future. Tabriz University of Medical Sciences 2023-03 2022-01-05 /pmc/articles/PMC10278211/ /pubmed/37342379 http://dx.doi.org/10.34172/apb.2023.041 Text en ©2023 The Authors. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, as long as the original authors and source are cited. No permission is required from the authors or the publishers. |
spellingShingle | Research Article Khordadmehr, Monireh Matin, Reyhaneh Baradaran, Behzad Baghbani, Elham Jigari-Asl, Farinaz Noorolyai, Saeed The Effect of miR-4800 Restoration on Proliferation and Migration of Human Breast Cancer Cells In Vitro |
title | The Effect of miR-4800 Restoration on Proliferation and Migration of Human Breast Cancer Cells In Vitro |
title_full | The Effect of miR-4800 Restoration on Proliferation and Migration of Human Breast Cancer Cells In Vitro |
title_fullStr | The Effect of miR-4800 Restoration on Proliferation and Migration of Human Breast Cancer Cells In Vitro |
title_full_unstemmed | The Effect of miR-4800 Restoration on Proliferation and Migration of Human Breast Cancer Cells In Vitro |
title_short | The Effect of miR-4800 Restoration on Proliferation and Migration of Human Breast Cancer Cells In Vitro |
title_sort | effect of mir-4800 restoration on proliferation and migration of human breast cancer cells in vitro |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10278211/ https://www.ncbi.nlm.nih.gov/pubmed/37342379 http://dx.doi.org/10.34172/apb.2023.041 |
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