The effect of background noise and its removal on the analysis of single-cell expression data

BACKGROUND: In droplet-based single-cell and single-nucleus RNA-seq experiments, not all reads associated with one cell barcode originate from the encapsulated cell. Such background noise is attributed to spillage from cell-free ambient RNA or barcode swapping events. RESULTS: Here, we characterize...

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Detalles Bibliográficos
Autores principales: Janssen, Philipp, Kliesmete, Zane, Vieth, Beate, Adiconis, Xian, Simmons, Sean, Marshall, Jamie, McCabe, Cristin, Heyn, Holger, Levin, Joshua Z., Enard, Wolfgang, Hellmann, Ines
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10278251/
https://www.ncbi.nlm.nih.gov/pubmed/37337297
http://dx.doi.org/10.1186/s13059-023-02978-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10278251/
https://www.ncbi.nlm.nih.gov/pubmed/37337297
http://dx.doi.org/10.1186/s13059-023-02978-x

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