Identification of hepatitis B virus genotypes detected in Lahaul & Spiti district of Himachal Pradesh, India

BACKGROUND & OBJECTIVES: Infection by hepatitis B virus (HBV) results in acute or chronic hepatitis. Based on sequence differences of eight per cent or more, HBV is divided into 10 genotypes (A to J) and 35 sub-genotypes. Molecular characterization of the circulating HBV genome has helped in und...

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Autores principales: Pacholi, Sanchita, Bishwal, Subasa Chandra, Barde, Pradip V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10278918/
https://www.ncbi.nlm.nih.gov/pubmed/37056078
http://dx.doi.org/10.4103/ijmr.ijmr_442_22
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author Pacholi, Sanchita
Bishwal, Subasa Chandra
Barde, Pradip V.
author_facet Pacholi, Sanchita
Bishwal, Subasa Chandra
Barde, Pradip V.
author_sort Pacholi, Sanchita
collection PubMed
description BACKGROUND & OBJECTIVES: Infection by hepatitis B virus (HBV) results in acute or chronic hepatitis. Based on sequence differences of eight per cent or more, HBV is divided into 10 genotypes (A to J) and 35 sub-genotypes. Molecular characterization of the circulating HBV genome has helped in understanding the epidemiology and its clinical importance. Spiti valley in Himachal Pradesh, which shares its border with Tibet, is one of the most HBV prevalent areas in India. Since information about the circulating genotype/s of HBV in this area is limited, this study was conducted to identify the circulating HBV genotypes. METHODS: The surface and partial reverse transcriptase gene regions were sequenced using 14 hepatitis B surface antigen-positive samples. RESULTS: Out of the 14 hepatitis B surface antigen-positive samples 11 sample gave quality sequence for further analysis. All the 11 samples belonged to subtype ayw2. The phylogenetic and recombination analysis revealed that five out of 11 samples were of genotype CD1 and the rest six were of genotype D3. INTERPRETATION & CONCLUSIONS: The CD1 recombinant sub-genotype might have immigrated during past or present transcontinental migration between the adjacent countries. Further studies using full-genome sequencing and high sample size will be helpful to understand this epidemiology and to combat the high prevalence of HBV in the area.
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spelling pubmed-102789182023-06-20 Identification of hepatitis B virus genotypes detected in Lahaul & Spiti district of Himachal Pradesh, India Pacholi, Sanchita Bishwal, Subasa Chandra Barde, Pradip V. Indian J Med Res Practice: Original article BACKGROUND & OBJECTIVES: Infection by hepatitis B virus (HBV) results in acute or chronic hepatitis. Based on sequence differences of eight per cent or more, HBV is divided into 10 genotypes (A to J) and 35 sub-genotypes. Molecular characterization of the circulating HBV genome has helped in understanding the epidemiology and its clinical importance. Spiti valley in Himachal Pradesh, which shares its border with Tibet, is one of the most HBV prevalent areas in India. Since information about the circulating genotype/s of HBV in this area is limited, this study was conducted to identify the circulating HBV genotypes. METHODS: The surface and partial reverse transcriptase gene regions were sequenced using 14 hepatitis B surface antigen-positive samples. RESULTS: Out of the 14 hepatitis B surface antigen-positive samples 11 sample gave quality sequence for further analysis. All the 11 samples belonged to subtype ayw2. The phylogenetic and recombination analysis revealed that five out of 11 samples were of genotype CD1 and the rest six were of genotype D3. INTERPRETATION & CONCLUSIONS: The CD1 recombinant sub-genotype might have immigrated during past or present transcontinental migration between the adjacent countries. Further studies using full-genome sequencing and high sample size will be helpful to understand this epidemiology and to combat the high prevalence of HBV in the area. Wolters Kluwer - Medknow 2022-12 2023-04-05 /pmc/articles/PMC10278918/ /pubmed/37056078 http://dx.doi.org/10.4103/ijmr.ijmr_442_22 Text en Copyright: © 2023 Indian Journal of Medical Research https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Practice: Original article
Pacholi, Sanchita
Bishwal, Subasa Chandra
Barde, Pradip V.
Identification of hepatitis B virus genotypes detected in Lahaul & Spiti district of Himachal Pradesh, India
title Identification of hepatitis B virus genotypes detected in Lahaul & Spiti district of Himachal Pradesh, India
title_full Identification of hepatitis B virus genotypes detected in Lahaul & Spiti district of Himachal Pradesh, India
title_fullStr Identification of hepatitis B virus genotypes detected in Lahaul & Spiti district of Himachal Pradesh, India
title_full_unstemmed Identification of hepatitis B virus genotypes detected in Lahaul & Spiti district of Himachal Pradesh, India
title_short Identification of hepatitis B virus genotypes detected in Lahaul & Spiti district of Himachal Pradesh, India
title_sort identification of hepatitis b virus genotypes detected in lahaul & spiti district of himachal pradesh, india
topic Practice: Original article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10278918/
https://www.ncbi.nlm.nih.gov/pubmed/37056078
http://dx.doi.org/10.4103/ijmr.ijmr_442_22
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