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Ethanol preservation and pretreatments facilitate quality DNA extractions in recalcitrant plant species

PREMISE: The preservation of plant tissues in ethanol is conventionally viewed as problematic. Here, we show that leaf preservation in ethanol combined with proteinase digestion can provide high‐quality DNA extracts. Additionally, as a pretreatment, ethanol can facilitate DNA extraction for recalcit...

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Detalles Bibliográficos
Autores principales: Johnson, Gabriel, Canty, Steven W. J., Lichter‐Marck, Isaac H., Wagner, Warren, Wen, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10278939/
https://www.ncbi.nlm.nih.gov/pubmed/37342166
http://dx.doi.org/10.1002/aps3.11519