Kiss1 gene expression, sperm indices and testicular histopathology following the administration of Hibiscus sabdariffa in rats

OBJECTIVE: This study investigated the expression of Kiss1 gene on the testis and the blood of Wistar rats, following the administration of methanolic extract of Hibiscus Sabdariffa (MEHS). METHODS: Fifteen (15) rats with an average weight of 204g were randomly divided into three (3) groups (A-C). Group A was given no treatment and served as the normal control group. Groups B and C were orally administered 200mg/kg and 400mg/kg of MEHS, respectively. The extract was administered once a day for 21 days. RESULTS: There was a significant increase in the relative testicular weight in group B and C compared to the control group (p=0.035). There was no significant difference in the sperm parameters, reproductive hormones, and antioxidant levels in all the treatment groups when compared to the control group (p>0.05). There is a significantly lower expression intensity of the Kiss1 gene in the blood in groups B (p=0.000) and C (p=0.017), compared to the control group. There is no difference in the relative intensity of Kiss1 gene expression in the testis of all the experimental groups (p=0.173). CONCLUSIONS: MEHS caused no histopathological changes on the testis at both doses. MEHS shows the potential of downregulating the expression of the Kiss1 gene in the blood. However, this effect lacks a regulatory mechanism on the reproductive hormones, sperm parameters, testicular morphology, and antioxidative levels.