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Fundus autofluorescence imaging using red excitation light

Retinal disease accounts significantly for visual impairment and blindness. An important role in the pathophysiology of retinal disease and aging is attributed to lipofuscin, a complex of fluorescent metabolites. Fundus autofluorescence (AF) imaging allows non-invasive mapping of lipofuscin and is a...

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Autores principales: Birtel, Johannes, Bauer, Tobias, Pauleikhoff, Laurenz, Rüber, Theodor, Gliem, Martin, Charbel Issa, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10279676/
https://www.ncbi.nlm.nih.gov/pubmed/37336979
http://dx.doi.org/10.1038/s41598-023-36217-x
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author Birtel, Johannes
Bauer, Tobias
Pauleikhoff, Laurenz
Rüber, Theodor
Gliem, Martin
Charbel Issa, Peter
author_facet Birtel, Johannes
Bauer, Tobias
Pauleikhoff, Laurenz
Rüber, Theodor
Gliem, Martin
Charbel Issa, Peter
author_sort Birtel, Johannes
collection PubMed
description Retinal disease accounts significantly for visual impairment and blindness. An important role in the pathophysiology of retinal disease and aging is attributed to lipofuscin, a complex of fluorescent metabolites. Fundus autofluorescence (AF) imaging allows non-invasive mapping of lipofuscin and is a key technology to diagnose and monitor retinal disease. However, currently used short-wavelength (SW) excitation light has several limitations, including glare and discomfort during image acquisition, reduced image quality in case of lens opacities, limited visualization of the central retina, and potential retinal light toxicity. Here, we establish a novel imaging modality which uses red excitation light (R-AF) and overcomes these drawbacks. R-AF images are high-quality, high-contrast fundus images and image interpretation may build on clinical experience due to similar appearance of pathology as on SW-AF images. Additionally, R-AF images may uncover disease features that previously remained undetected. The R-AF signal increases with higher abundance of lipofuscin and does not depend on photopigment bleaching or on the amount of macular pigment. Improved patient comfort, limited effect of cataract on image quality, and lack of safety concerns qualify R-AF for routine clinical monitoring, e.g. for patients with age-related macular degeneration, Stargardt disease, or for quantitative analysis of AF signal intensity.
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spelling pubmed-102796762023-06-21 Fundus autofluorescence imaging using red excitation light Birtel, Johannes Bauer, Tobias Pauleikhoff, Laurenz Rüber, Theodor Gliem, Martin Charbel Issa, Peter Sci Rep Article Retinal disease accounts significantly for visual impairment and blindness. An important role in the pathophysiology of retinal disease and aging is attributed to lipofuscin, a complex of fluorescent metabolites. Fundus autofluorescence (AF) imaging allows non-invasive mapping of lipofuscin and is a key technology to diagnose and monitor retinal disease. However, currently used short-wavelength (SW) excitation light has several limitations, including glare and discomfort during image acquisition, reduced image quality in case of lens opacities, limited visualization of the central retina, and potential retinal light toxicity. Here, we establish a novel imaging modality which uses red excitation light (R-AF) and overcomes these drawbacks. R-AF images are high-quality, high-contrast fundus images and image interpretation may build on clinical experience due to similar appearance of pathology as on SW-AF images. Additionally, R-AF images may uncover disease features that previously remained undetected. The R-AF signal increases with higher abundance of lipofuscin and does not depend on photopigment bleaching or on the amount of macular pigment. Improved patient comfort, limited effect of cataract on image quality, and lack of safety concerns qualify R-AF for routine clinical monitoring, e.g. for patients with age-related macular degeneration, Stargardt disease, or for quantitative analysis of AF signal intensity. Nature Publishing Group UK 2023-06-19 /pmc/articles/PMC10279676/ /pubmed/37336979 http://dx.doi.org/10.1038/s41598-023-36217-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Birtel, Johannes
Bauer, Tobias
Pauleikhoff, Laurenz
Rüber, Theodor
Gliem, Martin
Charbel Issa, Peter
Fundus autofluorescence imaging using red excitation light
title Fundus autofluorescence imaging using red excitation light
title_full Fundus autofluorescence imaging using red excitation light
title_fullStr Fundus autofluorescence imaging using red excitation light
title_full_unstemmed Fundus autofluorescence imaging using red excitation light
title_short Fundus autofluorescence imaging using red excitation light
title_sort fundus autofluorescence imaging using red excitation light
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10279676/
https://www.ncbi.nlm.nih.gov/pubmed/37336979
http://dx.doi.org/10.1038/s41598-023-36217-x
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