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Convergent evolution of plant pattern recognition receptors sensing cysteine-rich patterns from three microbial kingdoms
The Arabidopsis thaliana Receptor-Like Protein RLP30 contributes to immunity against the fungal pathogen Sclerotinia sclerotiorum. Here we identify the RLP30-ligand as a small cysteine-rich protein (SCP) that occurs in many fungi and oomycetes and is also recognized by the Nicotiana benthamiana RLP...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10279758/ https://www.ncbi.nlm.nih.gov/pubmed/37336953 http://dx.doi.org/10.1038/s41467-023-39208-8 |
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author | Yang, Yuankun Steidele, Christina E. Rössner, Clemens Löffelhardt, Birgit Kolb, Dagmar Leisen, Thomas Zhang, Weiguo Ludwig, Christina Felix, Georg Seidl, Michael F. Becker, Annette Nürnberger, Thorsten Hahn, Matthias Gust, Bertolt Gross, Harald Hückelhoven, Ralph Gust, Andrea A. |
author_facet | Yang, Yuankun Steidele, Christina E. Rössner, Clemens Löffelhardt, Birgit Kolb, Dagmar Leisen, Thomas Zhang, Weiguo Ludwig, Christina Felix, Georg Seidl, Michael F. Becker, Annette Nürnberger, Thorsten Hahn, Matthias Gust, Bertolt Gross, Harald Hückelhoven, Ralph Gust, Andrea A. |
author_sort | Yang, Yuankun |
collection | PubMed |
description | The Arabidopsis thaliana Receptor-Like Protein RLP30 contributes to immunity against the fungal pathogen Sclerotinia sclerotiorum. Here we identify the RLP30-ligand as a small cysteine-rich protein (SCP) that occurs in many fungi and oomycetes and is also recognized by the Nicotiana benthamiana RLP RE02. However, RLP30 and RE02 share little sequence similarity and respond to different parts of the native/folded protein. Moreover, some Brassicaceae other than Arabidopsis also respond to a linear SCP peptide instead of the folded protein, suggesting that SCP is an eminent immune target that led to the convergent evolution of distinct immune receptors in plants. Surprisingly, RLP30 shows a second ligand specificity for a SCP-nonhomologous protein secreted by bacterial Pseudomonads. RLP30 expression in N. tabacum results in quantitatively lower susceptibility to bacterial, fungal and oomycete pathogens, thus demonstrating that detection of immunogenic patterns by Arabidopsis RLP30 is involved in defense against pathogens from three microbial kingdoms. |
format | Online Article Text |
id | pubmed-10279758 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-102797582023-06-21 Convergent evolution of plant pattern recognition receptors sensing cysteine-rich patterns from three microbial kingdoms Yang, Yuankun Steidele, Christina E. Rössner, Clemens Löffelhardt, Birgit Kolb, Dagmar Leisen, Thomas Zhang, Weiguo Ludwig, Christina Felix, Georg Seidl, Michael F. Becker, Annette Nürnberger, Thorsten Hahn, Matthias Gust, Bertolt Gross, Harald Hückelhoven, Ralph Gust, Andrea A. Nat Commun Article The Arabidopsis thaliana Receptor-Like Protein RLP30 contributes to immunity against the fungal pathogen Sclerotinia sclerotiorum. Here we identify the RLP30-ligand as a small cysteine-rich protein (SCP) that occurs in many fungi and oomycetes and is also recognized by the Nicotiana benthamiana RLP RE02. However, RLP30 and RE02 share little sequence similarity and respond to different parts of the native/folded protein. Moreover, some Brassicaceae other than Arabidopsis also respond to a linear SCP peptide instead of the folded protein, suggesting that SCP is an eminent immune target that led to the convergent evolution of distinct immune receptors in plants. Surprisingly, RLP30 shows a second ligand specificity for a SCP-nonhomologous protein secreted by bacterial Pseudomonads. RLP30 expression in N. tabacum results in quantitatively lower susceptibility to bacterial, fungal and oomycete pathogens, thus demonstrating that detection of immunogenic patterns by Arabidopsis RLP30 is involved in defense against pathogens from three microbial kingdoms. Nature Publishing Group UK 2023-06-19 /pmc/articles/PMC10279758/ /pubmed/37336953 http://dx.doi.org/10.1038/s41467-023-39208-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Yang, Yuankun Steidele, Christina E. Rössner, Clemens Löffelhardt, Birgit Kolb, Dagmar Leisen, Thomas Zhang, Weiguo Ludwig, Christina Felix, Georg Seidl, Michael F. Becker, Annette Nürnberger, Thorsten Hahn, Matthias Gust, Bertolt Gross, Harald Hückelhoven, Ralph Gust, Andrea A. Convergent evolution of plant pattern recognition receptors sensing cysteine-rich patterns from three microbial kingdoms |
title | Convergent evolution of plant pattern recognition receptors sensing cysteine-rich patterns from three microbial kingdoms |
title_full | Convergent evolution of plant pattern recognition receptors sensing cysteine-rich patterns from three microbial kingdoms |
title_fullStr | Convergent evolution of plant pattern recognition receptors sensing cysteine-rich patterns from three microbial kingdoms |
title_full_unstemmed | Convergent evolution of plant pattern recognition receptors sensing cysteine-rich patterns from three microbial kingdoms |
title_short | Convergent evolution of plant pattern recognition receptors sensing cysteine-rich patterns from three microbial kingdoms |
title_sort | convergent evolution of plant pattern recognition receptors sensing cysteine-rich patterns from three microbial kingdoms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10279758/ https://www.ncbi.nlm.nih.gov/pubmed/37336953 http://dx.doi.org/10.1038/s41467-023-39208-8 |
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