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The consistent application of hydrogen peroxide controls biofilm growth and removes Vermamoeba vermiformis from multi-kingdom in-vitro dental unit water biofilms

The water systems inside a dental unit are known to be contaminated with a multi-kingdom biofilm encompassing bacteria, fungi, viruses and protozoa. Aerosolization of these micro-organisms can potentially create a health hazard for both dental staff and the patient. Very little is known on the effic...

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Autores principales: Hoogenkamp, Michel A., Mazurel, Danuta, Deutekom-Mulder, Elly, de Soet, Johannes J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10279787/
https://www.ncbi.nlm.nih.gov/pubmed/37346320
http://dx.doi.org/10.1016/j.bioflm.2023.100132
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author Hoogenkamp, Michel A.
Mazurel, Danuta
Deutekom-Mulder, Elly
de Soet, Johannes J.
author_facet Hoogenkamp, Michel A.
Mazurel, Danuta
Deutekom-Mulder, Elly
de Soet, Johannes J.
author_sort Hoogenkamp, Michel A.
collection PubMed
description The water systems inside a dental unit are known to be contaminated with a multi-kingdom biofilm encompassing bacteria, fungi, viruses and protozoa. Aerosolization of these micro-organisms can potentially create a health hazard for both dental staff and the patient. Very little is known on the efficacy of dental unit disinfection products against amoeba. In this study we have examined the effect of four different treatment regimens, with the hydrogen peroxide (H(2)O(2)) containing product Oxygenal, on an in-vitro multi-kingdom dental unit water system (DUWS) biofilm. The treatment efficacy was assessed in time using heterotrophic plate counts, the bacterial 16S rDNA, fungal 18S rDNA gene load and the number of genomic units for Legionella spp. the amoeba Vermamoeba vermiformis. The results indicated that a daily treatment of the DUWS with a low dose H(2)O(2) (0.02% for 5 h), combined with a weekly shock dose (0.25% H(2)O(2), 30 min) is necessary to reduce the heterotrophic plate count of a severely contaminated DUWS (>10(6) CFU.mL(−1)) to below 100 CFU.mL(−1). A daily treatment with a low dose hydrogen peroxide alone, is sufficient for the statistically significant reduction of the total amount of bacterial 16S rDNA gene, Legionella spp. and Vermamoeba vermiformis load (p < 0.005). Also shown is that even though hydrogen peroxide does not kill the trophozoite nor the cysts of V. vermiformis, it does however result in the detachment of the trophozoite form of this amoeba from the DUWS biofilm and hereby ultimately removing the amoeba from the system.
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spelling pubmed-102797872023-06-21 The consistent application of hydrogen peroxide controls biofilm growth and removes Vermamoeba vermiformis from multi-kingdom in-vitro dental unit water biofilms Hoogenkamp, Michel A. Mazurel, Danuta Deutekom-Mulder, Elly de Soet, Johannes J. Biofilm Article The water systems inside a dental unit are known to be contaminated with a multi-kingdom biofilm encompassing bacteria, fungi, viruses and protozoa. Aerosolization of these micro-organisms can potentially create a health hazard for both dental staff and the patient. Very little is known on the efficacy of dental unit disinfection products against amoeba. In this study we have examined the effect of four different treatment regimens, with the hydrogen peroxide (H(2)O(2)) containing product Oxygenal, on an in-vitro multi-kingdom dental unit water system (DUWS) biofilm. The treatment efficacy was assessed in time using heterotrophic plate counts, the bacterial 16S rDNA, fungal 18S rDNA gene load and the number of genomic units for Legionella spp. the amoeba Vermamoeba vermiformis. The results indicated that a daily treatment of the DUWS with a low dose H(2)O(2) (0.02% for 5 h), combined with a weekly shock dose (0.25% H(2)O(2), 30 min) is necessary to reduce the heterotrophic plate count of a severely contaminated DUWS (>10(6) CFU.mL(−1)) to below 100 CFU.mL(−1). A daily treatment with a low dose hydrogen peroxide alone, is sufficient for the statistically significant reduction of the total amount of bacterial 16S rDNA gene, Legionella spp. and Vermamoeba vermiformis load (p < 0.005). Also shown is that even though hydrogen peroxide does not kill the trophozoite nor the cysts of V. vermiformis, it does however result in the detachment of the trophozoite form of this amoeba from the DUWS biofilm and hereby ultimately removing the amoeba from the system. Elsevier 2023-05-25 /pmc/articles/PMC10279787/ /pubmed/37346320 http://dx.doi.org/10.1016/j.bioflm.2023.100132 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Hoogenkamp, Michel A.
Mazurel, Danuta
Deutekom-Mulder, Elly
de Soet, Johannes J.
The consistent application of hydrogen peroxide controls biofilm growth and removes Vermamoeba vermiformis from multi-kingdom in-vitro dental unit water biofilms
title The consistent application of hydrogen peroxide controls biofilm growth and removes Vermamoeba vermiformis from multi-kingdom in-vitro dental unit water biofilms
title_full The consistent application of hydrogen peroxide controls biofilm growth and removes Vermamoeba vermiformis from multi-kingdom in-vitro dental unit water biofilms
title_fullStr The consistent application of hydrogen peroxide controls biofilm growth and removes Vermamoeba vermiformis from multi-kingdom in-vitro dental unit water biofilms
title_full_unstemmed The consistent application of hydrogen peroxide controls biofilm growth and removes Vermamoeba vermiformis from multi-kingdom in-vitro dental unit water biofilms
title_short The consistent application of hydrogen peroxide controls biofilm growth and removes Vermamoeba vermiformis from multi-kingdom in-vitro dental unit water biofilms
title_sort consistent application of hydrogen peroxide controls biofilm growth and removes vermamoeba vermiformis from multi-kingdom in-vitro dental unit water biofilms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10279787/
https://www.ncbi.nlm.nih.gov/pubmed/37346320
http://dx.doi.org/10.1016/j.bioflm.2023.100132
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