pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination

Streptococcus pyogenes, or Group A Streptococcus (GAS), is a major human pathogen for which genetic manipulation remains an ongoing challenge. We created a new temperature-sensitive plasmid pBFK expressing spectinomycin resistance adapted to homologous recombination procedure to perform a complete g...

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Detalles Bibliográficos
Autores principales: Boukthir, S., Gaudu, P., Faili, A., Kayal, S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10279790/
https://www.ncbi.nlm.nih.gov/pubmed/37346331
http://dx.doi.org/10.1016/j.heliyon.2023.e16720
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author Boukthir, S.
Gaudu, P.
Faili, A.
Kayal, S.
author_facet Boukthir, S.
Gaudu, P.
Faili, A.
Kayal, S.
author_sort Boukthir, S.
collection PubMed
description Streptococcus pyogenes, or Group A Streptococcus (GAS), is a major human pathogen for which genetic manipulation remains an ongoing challenge. We created a new temperature-sensitive plasmid pBFK expressing spectinomycin resistance adapted to homologous recombination procedure to perform a complete gene deletion in GAS. Herein the mutagenesis strategy with pBFK was performed in a highly virulent GAS emm3 genotype.
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spelling pubmed-102797902023-06-21 pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination Boukthir, S. Gaudu, P. Faili, A. Kayal, S. Heliyon Research Article Streptococcus pyogenes, or Group A Streptococcus (GAS), is a major human pathogen for which genetic manipulation remains an ongoing challenge. We created a new temperature-sensitive plasmid pBFK expressing spectinomycin resistance adapted to homologous recombination procedure to perform a complete gene deletion in GAS. Herein the mutagenesis strategy with pBFK was performed in a highly virulent GAS emm3 genotype. Elsevier 2023-05-29 /pmc/articles/PMC10279790/ /pubmed/37346331 http://dx.doi.org/10.1016/j.heliyon.2023.e16720 Text en © 2023 Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Boukthir, S.
Gaudu, P.
Faili, A.
Kayal, S.
pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination
title pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination
title_full pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination
title_fullStr pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination
title_full_unstemmed pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination
title_short pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination
title_sort pbfk, a new thermosensitive shuttle vector for streptococcus pyogenes gene deletion by homologous recombination
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10279790/
https://www.ncbi.nlm.nih.gov/pubmed/37346331
http://dx.doi.org/10.1016/j.heliyon.2023.e16720
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