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pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination
Streptococcus pyogenes, or Group A Streptococcus (GAS), is a major human pathogen for which genetic manipulation remains an ongoing challenge. We created a new temperature-sensitive plasmid pBFK expressing spectinomycin resistance adapted to homologous recombination procedure to perform a complete g...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10279790/ https://www.ncbi.nlm.nih.gov/pubmed/37346331 http://dx.doi.org/10.1016/j.heliyon.2023.e16720 |