Cargando…

Schistosoma japonicum-derived peptide SJMHE1 ameliorates allergic symptoms and responses in mice with allergic rhinitis

Helminth derived excretory/secretory molecules have shown efficacy in the treatment of allergic asthma in mice, but their roles in allergic rhinitis (AR) are little known. In this study, we aimed to determine the intervention effect of SJMHE1, a Schistosoma japonicum derived small molecular peptide,...

Descripción completa

Detalles Bibliográficos
Autores principales: Gao, Xuerong, Mao, Chaoming, Zheng, Tingting, Xu, Xiaowei, Luo, Xinkai, Zhang, Shan, Liu, Jiameng, Wang, Xuefeng, Chen, Xiaojun, Dong, Liyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10279851/
https://www.ncbi.nlm.nih.gov/pubmed/37346033
http://dx.doi.org/10.3389/fcimb.2023.1143950
_version_ 1785060677304451072
author Gao, Xuerong
Mao, Chaoming
Zheng, Tingting
Xu, Xiaowei
Luo, Xinkai
Zhang, Shan
Liu, Jiameng
Wang, Xuefeng
Chen, Xiaojun
Dong, Liyang
author_facet Gao, Xuerong
Mao, Chaoming
Zheng, Tingting
Xu, Xiaowei
Luo, Xinkai
Zhang, Shan
Liu, Jiameng
Wang, Xuefeng
Chen, Xiaojun
Dong, Liyang
author_sort Gao, Xuerong
collection PubMed
description Helminth derived excretory/secretory molecules have shown efficacy in the treatment of allergic asthma in mice, but their roles in allergic rhinitis (AR) are little known. In this study, we aimed to determine the intervention effect of SJMHE1, a Schistosoma japonicum derived small molecular peptide, on ovalbumin (OVA)-induced AR mice and investigate its possible mechanism. AR was induced in BALB/c mice, following which the mice were treated with phosphate-buffered saline (PBS), OVA(323-339) and SJMHE1 respectively. SJMHE1 treatment improved clinical symptoms (rubbing and sneezing), suppressed infiltrates of inflammatory cells and eosinophils in nasal mucosa, modulated the production of type-2 (IL-4 and IL-13) and anti-inflammatory (IL-10) cytokines in the nasal lavage fluids (NLF), spleen, and serum. To investigate the underlying mechanism, fluorescein isothiocyanate (FITC)-labeled SJMHE1 was subcutaneously injected into AR mice, and we found that the FITC-SJMHE1 could accumulate in spleen, but not in nasal mucosa. FITC-SJMHE1 mainly bound to CD19 positive cells (B cells), and the SJMHE1 treatment significantly increased the proportion of regulatory B cells (Bregs) and B10 cells, along with the enhancement of PR domain containing protein 1 (Prdm1) protein levels. SJMHE1 may alleviate AR by upregulating Bregs, and has great potential as a new avenue for the AR treatment.
format Online
Article
Text
id pubmed-10279851
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-102798512023-06-21 Schistosoma japonicum-derived peptide SJMHE1 ameliorates allergic symptoms and responses in mice with allergic rhinitis Gao, Xuerong Mao, Chaoming Zheng, Tingting Xu, Xiaowei Luo, Xinkai Zhang, Shan Liu, Jiameng Wang, Xuefeng Chen, Xiaojun Dong, Liyang Front Cell Infect Microbiol Cellular and Infection Microbiology Helminth derived excretory/secretory molecules have shown efficacy in the treatment of allergic asthma in mice, but their roles in allergic rhinitis (AR) are little known. In this study, we aimed to determine the intervention effect of SJMHE1, a Schistosoma japonicum derived small molecular peptide, on ovalbumin (OVA)-induced AR mice and investigate its possible mechanism. AR was induced in BALB/c mice, following which the mice were treated with phosphate-buffered saline (PBS), OVA(323-339) and SJMHE1 respectively. SJMHE1 treatment improved clinical symptoms (rubbing and sneezing), suppressed infiltrates of inflammatory cells and eosinophils in nasal mucosa, modulated the production of type-2 (IL-4 and IL-13) and anti-inflammatory (IL-10) cytokines in the nasal lavage fluids (NLF), spleen, and serum. To investigate the underlying mechanism, fluorescein isothiocyanate (FITC)-labeled SJMHE1 was subcutaneously injected into AR mice, and we found that the FITC-SJMHE1 could accumulate in spleen, but not in nasal mucosa. FITC-SJMHE1 mainly bound to CD19 positive cells (B cells), and the SJMHE1 treatment significantly increased the proportion of regulatory B cells (Bregs) and B10 cells, along with the enhancement of PR domain containing protein 1 (Prdm1) protein levels. SJMHE1 may alleviate AR by upregulating Bregs, and has great potential as a new avenue for the AR treatment. Frontiers Media S.A. 2023-06-06 /pmc/articles/PMC10279851/ /pubmed/37346033 http://dx.doi.org/10.3389/fcimb.2023.1143950 Text en Copyright © 2023 Gao, Mao, Zheng, Xu, Luo, Zhang, Liu, Wang, Chen and Dong https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Gao, Xuerong
Mao, Chaoming
Zheng, Tingting
Xu, Xiaowei
Luo, Xinkai
Zhang, Shan
Liu, Jiameng
Wang, Xuefeng
Chen, Xiaojun
Dong, Liyang
Schistosoma japonicum-derived peptide SJMHE1 ameliorates allergic symptoms and responses in mice with allergic rhinitis
title Schistosoma japonicum-derived peptide SJMHE1 ameliorates allergic symptoms and responses in mice with allergic rhinitis
title_full Schistosoma japonicum-derived peptide SJMHE1 ameliorates allergic symptoms and responses in mice with allergic rhinitis
title_fullStr Schistosoma japonicum-derived peptide SJMHE1 ameliorates allergic symptoms and responses in mice with allergic rhinitis
title_full_unstemmed Schistosoma japonicum-derived peptide SJMHE1 ameliorates allergic symptoms and responses in mice with allergic rhinitis
title_short Schistosoma japonicum-derived peptide SJMHE1 ameliorates allergic symptoms and responses in mice with allergic rhinitis
title_sort schistosoma japonicum-derived peptide sjmhe1 ameliorates allergic symptoms and responses in mice with allergic rhinitis
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10279851/
https://www.ncbi.nlm.nih.gov/pubmed/37346033
http://dx.doi.org/10.3389/fcimb.2023.1143950
work_keys_str_mv AT gaoxuerong schistosomajaponicumderivedpeptidesjmhe1amelioratesallergicsymptomsandresponsesinmicewithallergicrhinitis
AT maochaoming schistosomajaponicumderivedpeptidesjmhe1amelioratesallergicsymptomsandresponsesinmicewithallergicrhinitis
AT zhengtingting schistosomajaponicumderivedpeptidesjmhe1amelioratesallergicsymptomsandresponsesinmicewithallergicrhinitis
AT xuxiaowei schistosomajaponicumderivedpeptidesjmhe1amelioratesallergicsymptomsandresponsesinmicewithallergicrhinitis
AT luoxinkai schistosomajaponicumderivedpeptidesjmhe1amelioratesallergicsymptomsandresponsesinmicewithallergicrhinitis
AT zhangshan schistosomajaponicumderivedpeptidesjmhe1amelioratesallergicsymptomsandresponsesinmicewithallergicrhinitis
AT liujiameng schistosomajaponicumderivedpeptidesjmhe1amelioratesallergicsymptomsandresponsesinmicewithallergicrhinitis
AT wangxuefeng schistosomajaponicumderivedpeptidesjmhe1amelioratesallergicsymptomsandresponsesinmicewithallergicrhinitis
AT chenxiaojun schistosomajaponicumderivedpeptidesjmhe1amelioratesallergicsymptomsandresponsesinmicewithallergicrhinitis
AT dongliyang schistosomajaponicumderivedpeptidesjmhe1amelioratesallergicsymptomsandresponsesinmicewithallergicrhinitis