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Proangiogenic role of circRNA‐007371 in liver fibrosis
Circular RNAs (circRNAs) are crucially involved in cancers as competing endogenous RNA (ceRNA) or microRNA (miRNA) sponges. However, the function and mechanism of circRNAs in liver fibrosis remain unknown and are the focus of this study. Murine fibrotic models were induced by thioacetamide (TAA) or...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10280135/ https://www.ncbi.nlm.nih.gov/pubmed/36854930 http://dx.doi.org/10.1111/cpr.13432 |
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author | Zhao, Chong Qian, Shuaijie Tai, Yang Guo, Yangkun Tang, Chengwei Huang, Zhiyin Gao, Jinhang |
author_facet | Zhao, Chong Qian, Shuaijie Tai, Yang Guo, Yangkun Tang, Chengwei Huang, Zhiyin Gao, Jinhang |
author_sort | Zhao, Chong |
collection | PubMed |
description | Circular RNAs (circRNAs) are crucially involved in cancers as competing endogenous RNA (ceRNA) or microRNA (miRNA) sponges. However, the function and mechanism of circRNAs in liver fibrosis remain unknown and are the focus of this study. Murine fibrotic models were induced by thioacetamide (TAA) or carbon tetrachloride (CCl(4)). Increased angiogenesis is accompanied by liver fibrosis in TAA‐ and CCl(4)‐induced murine fibrotic livers. circRNA microarray and argonaute 2 (AGO2)‐RNA immunoprecipitation (RIP) sequencing (AGO2‐RIP sequencing) were performed in murine livers to screen for functional circRNAs. Compared to control livers, 86 differentially expressed circRNAs were obtained in TAA‐induced murine fibrotic livers using circRNA microarray. In addition, 551 circRNAs were explored by AGO2‐RIP sequencing of murine fibrotic livers. The circRNA‐007371 was then selected and verified for back‐spliced junction, resistance to RNase R, and loop formation. In vitro, murine hemangioendothelioma endothelial (EOMA) cells were transfected with circRNA‐007371 overexpressing plasmid or empty plasmid. circRNA‐007371 overexpression promoted tube formation, migration, and cell proliferation of EOMA cells. RNA sequencing and miRNA sequencing were then performed to explore the mechanism of the proangiogenic effects of circRNA‐007371. circRNA‐007371 promotes liver fibrosis via miRNA sponges or ceRNA mechanisms. Stag1, the parent gene of circRNA‐007371, may play a significant role in proangiogenic progression. In conclusion, circRNA‐007371 enhances angiogenesis via a miRNA sponge mechanism in liver fibrosis. The antiangiogenic effect of circRNA‐007371 inhibition may provide a new strategy for treating patients with liver cirrhosis. |
format | Online Article Text |
id | pubmed-10280135 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102801352023-06-21 Proangiogenic role of circRNA‐007371 in liver fibrosis Zhao, Chong Qian, Shuaijie Tai, Yang Guo, Yangkun Tang, Chengwei Huang, Zhiyin Gao, Jinhang Cell Prolif Original Articles Circular RNAs (circRNAs) are crucially involved in cancers as competing endogenous RNA (ceRNA) or microRNA (miRNA) sponges. However, the function and mechanism of circRNAs in liver fibrosis remain unknown and are the focus of this study. Murine fibrotic models were induced by thioacetamide (TAA) or carbon tetrachloride (CCl(4)). Increased angiogenesis is accompanied by liver fibrosis in TAA‐ and CCl(4)‐induced murine fibrotic livers. circRNA microarray and argonaute 2 (AGO2)‐RNA immunoprecipitation (RIP) sequencing (AGO2‐RIP sequencing) were performed in murine livers to screen for functional circRNAs. Compared to control livers, 86 differentially expressed circRNAs were obtained in TAA‐induced murine fibrotic livers using circRNA microarray. In addition, 551 circRNAs were explored by AGO2‐RIP sequencing of murine fibrotic livers. The circRNA‐007371 was then selected and verified for back‐spliced junction, resistance to RNase R, and loop formation. In vitro, murine hemangioendothelioma endothelial (EOMA) cells were transfected with circRNA‐007371 overexpressing plasmid or empty plasmid. circRNA‐007371 overexpression promoted tube formation, migration, and cell proliferation of EOMA cells. RNA sequencing and miRNA sequencing were then performed to explore the mechanism of the proangiogenic effects of circRNA‐007371. circRNA‐007371 promotes liver fibrosis via miRNA sponges or ceRNA mechanisms. Stag1, the parent gene of circRNA‐007371, may play a significant role in proangiogenic progression. In conclusion, circRNA‐007371 enhances angiogenesis via a miRNA sponge mechanism in liver fibrosis. The antiangiogenic effect of circRNA‐007371 inhibition may provide a new strategy for treating patients with liver cirrhosis. John Wiley and Sons Inc. 2023-02-28 /pmc/articles/PMC10280135/ /pubmed/36854930 http://dx.doi.org/10.1111/cpr.13432 Text en © 2023 The Authors. Cell Proliferation published by Beijing Institute for Stem Cell and Regenerative Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Zhao, Chong Qian, Shuaijie Tai, Yang Guo, Yangkun Tang, Chengwei Huang, Zhiyin Gao, Jinhang Proangiogenic role of circRNA‐007371 in liver fibrosis |
title | Proangiogenic role of circRNA‐007371 in liver fibrosis |
title_full | Proangiogenic role of circRNA‐007371 in liver fibrosis |
title_fullStr | Proangiogenic role of circRNA‐007371 in liver fibrosis |
title_full_unstemmed | Proangiogenic role of circRNA‐007371 in liver fibrosis |
title_short | Proangiogenic role of circRNA‐007371 in liver fibrosis |
title_sort | proangiogenic role of circrna‐007371 in liver fibrosis |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10280135/ https://www.ncbi.nlm.nih.gov/pubmed/36854930 http://dx.doi.org/10.1111/cpr.13432 |
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