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Establishment and assessment of a preclinical model of acute kidney injury induced by contrast media combined acute myocardial ischemia reperfusion surgery
Acute kidney injury (AKI) is a common complication after acute myocardial infarction (AMI) in clinical practice, and the majority of previous preclinical models were induced by a single factor. The objective of the present study was to establish a stable preclinic model of AKI induced by contrast me...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10280325/ https://www.ncbi.nlm.nih.gov/pubmed/37346411 http://dx.doi.org/10.3892/etm.2023.12020 |
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author | Yu, Sijia Dong, Xiaoli Lai, Wenguang Lu, Hongyu Xie, Yun Xu, Jun-Yan Zeng, Yewen Han, Kedong Liang, Jinqiang Liu, Jin Liu, Yong Chen, Jiyan |
author_facet | Yu, Sijia Dong, Xiaoli Lai, Wenguang Lu, Hongyu Xie, Yun Xu, Jun-Yan Zeng, Yewen Han, Kedong Liang, Jinqiang Liu, Jin Liu, Yong Chen, Jiyan |
author_sort | Yu, Sijia |
collection | PubMed |
description | Acute kidney injury (AKI) is a common complication after acute myocardial infarction (AMI) in clinical practice, and the majority of previous preclinical models were induced by a single factor. The objective of the present study was to establish a stable preclinic model of AKI induced by contrast media (CM) with acute myocardial ischemia reperfusion surgery and to identify the effect of oxidative stress on kidney injury. Rats were treated individually or with CM or myocardial ischemia reperfusion surgery. Renal baseline and AKI parameters, the level of oxidative stress and histopathological images were examined along with AKI biomarkers. Results showed the incidence of AKI in the CM group and ischemia reperfusion injury (IRI) group was 40%, χ(2) test (P<0.05 vs. CM-IRI) and 35%, χ(2) test (P<0.05 vs. CM-IRI) and the combination group had the highest incidence rate 75%. IRI surgery combined with CM diminished kidney function and induced oxidative stress by increasing creatinine, blood urea nitrogen and reactive oxygen species levels. Western blotting showed that the early AKI biomarker of NGAL and KIM-1 increased and that the combination group had the highest value. Pathology damage exhibited severe kidney damage in the combination group compared with other control groups. The present research established a reliable preclinic model of post-AMI AKI with a stable and high postoperative AKI rate. Additionally, CM was demonstrated to exacerbate AKI caused by acute myocardial infarction through oxidative stress and, thus, oxidative stress may be a potential therapeutic target. |
format | Online Article Text |
id | pubmed-10280325 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-102803252023-06-21 Establishment and assessment of a preclinical model of acute kidney injury induced by contrast media combined acute myocardial ischemia reperfusion surgery Yu, Sijia Dong, Xiaoli Lai, Wenguang Lu, Hongyu Xie, Yun Xu, Jun-Yan Zeng, Yewen Han, Kedong Liang, Jinqiang Liu, Jin Liu, Yong Chen, Jiyan Exp Ther Med Articles Acute kidney injury (AKI) is a common complication after acute myocardial infarction (AMI) in clinical practice, and the majority of previous preclinical models were induced by a single factor. The objective of the present study was to establish a stable preclinic model of AKI induced by contrast media (CM) with acute myocardial ischemia reperfusion surgery and to identify the effect of oxidative stress on kidney injury. Rats were treated individually or with CM or myocardial ischemia reperfusion surgery. Renal baseline and AKI parameters, the level of oxidative stress and histopathological images were examined along with AKI biomarkers. Results showed the incidence of AKI in the CM group and ischemia reperfusion injury (IRI) group was 40%, χ(2) test (P<0.05 vs. CM-IRI) and 35%, χ(2) test (P<0.05 vs. CM-IRI) and the combination group had the highest incidence rate 75%. IRI surgery combined with CM diminished kidney function and induced oxidative stress by increasing creatinine, blood urea nitrogen and reactive oxygen species levels. Western blotting showed that the early AKI biomarker of NGAL and KIM-1 increased and that the combination group had the highest value. Pathology damage exhibited severe kidney damage in the combination group compared with other control groups. The present research established a reliable preclinic model of post-AMI AKI with a stable and high postoperative AKI rate. Additionally, CM was demonstrated to exacerbate AKI caused by acute myocardial infarction through oxidative stress and, thus, oxidative stress may be a potential therapeutic target. D.A. Spandidos 2023-05-15 /pmc/articles/PMC10280325/ /pubmed/37346411 http://dx.doi.org/10.3892/etm.2023.12020 Text en Copyright: © Yu et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Yu, Sijia Dong, Xiaoli Lai, Wenguang Lu, Hongyu Xie, Yun Xu, Jun-Yan Zeng, Yewen Han, Kedong Liang, Jinqiang Liu, Jin Liu, Yong Chen, Jiyan Establishment and assessment of a preclinical model of acute kidney injury induced by contrast media combined acute myocardial ischemia reperfusion surgery |
title | Establishment and assessment of a preclinical model of acute kidney injury induced by contrast media combined acute myocardial ischemia reperfusion surgery |
title_full | Establishment and assessment of a preclinical model of acute kidney injury induced by contrast media combined acute myocardial ischemia reperfusion surgery |
title_fullStr | Establishment and assessment of a preclinical model of acute kidney injury induced by contrast media combined acute myocardial ischemia reperfusion surgery |
title_full_unstemmed | Establishment and assessment of a preclinical model of acute kidney injury induced by contrast media combined acute myocardial ischemia reperfusion surgery |
title_short | Establishment and assessment of a preclinical model of acute kidney injury induced by contrast media combined acute myocardial ischemia reperfusion surgery |
title_sort | establishment and assessment of a preclinical model of acute kidney injury induced by contrast media combined acute myocardial ischemia reperfusion surgery |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10280325/ https://www.ncbi.nlm.nih.gov/pubmed/37346411 http://dx.doi.org/10.3892/etm.2023.12020 |
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