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LncRNA landscape and associated ceRNA network in placental villus of unexplained recurrent spontaneous abortion

BACKGROUND: Unexplained recurrent spontaneous abortion (URSA) is one of the most challenging conditions frustrates women of childbearing age profoundly. The gene expression patterns and biological characteristics of placental villus in patients with URSA remain largely unknown. The aim of our study...

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Autores principales: Tang, Minyue, Li, Qingfang, Wan, Shan, Chen, Qingqing, Feng, Shujun, You, Jiali, Wang, Wei, Zhu, Yimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10280933/
https://www.ncbi.nlm.nih.gov/pubmed/37340405
http://dx.doi.org/10.1186/s12958-023-01107-4
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author Tang, Minyue
Li, Qingfang
Wan, Shan
Chen, Qingqing
Feng, Shujun
You, Jiali
Wang, Wei
Zhu, Yimin
author_facet Tang, Minyue
Li, Qingfang
Wan, Shan
Chen, Qingqing
Feng, Shujun
You, Jiali
Wang, Wei
Zhu, Yimin
author_sort Tang, Minyue
collection PubMed
description BACKGROUND: Unexplained recurrent spontaneous abortion (URSA) is one of the most challenging conditions frustrates women of childbearing age profoundly. The gene expression patterns and biological characteristics of placental villus in patients with URSA remain largely unknown. The aim of our study was to identify potential lncRNAs as well as their action mechanisms in URSA. METHOD: The ceRNA microarray was used to identify the mRNA and lncRNA expression profiles of URSA patients and normal pregnancy. Functional enrichment analyses for differentially expressed mRNAs in URSA were performed. Protein-protein interaction analysis of differentially expressed mRNAs was performed to identify hub genes and key modules. Subsequently, the co-dysregulated ceRNA network of URSA was established, and the enrichment analyses for the mRNAs in the ceRNA network was implemented. qRT-PCR was performed to validated the expression of key ENST00000429019 and mRNAs in URSA. RESULTS: We found that URSA placental villus have distinct mRNA and lncRNA expression profiles through ceRNA microarray, with a total of 347 mRNAs and 361 lncRNAs differentially expressed compared with controls. The functional enrichment analysis revealed that ncRNA processing, DNA replication, cell cycle, apoptosis, cytokine-mediated signaling pathway, ECM-receptor interaction were the potentially disrupted pathways in URSA patients. Then we constructed a co-dysregulated ceRNA network and found differentially expressed mRNAs were regulated by a small fraction of hub lncRNAs. Finally, we found a key network of ENST00000429019 and three cell proliferation or apoptosis related key mRNAs (CDCA3, KIFC1, NCAPH), and validated their expression and regulation in tissue and cellular levels. CONCLUSIONS: This study identified a key ceRNA network, which might take part in URSA and correlate with cell proliferation and apoptosis. Optimistically, this study may deepen our apprehensions about the underlying molecular and biological causes of URSA and provide an important theoretical basis for future therapeutic strategies for patients with URSA. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12958-023-01107-4.
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spelling pubmed-102809332023-06-21 LncRNA landscape and associated ceRNA network in placental villus of unexplained recurrent spontaneous abortion Tang, Minyue Li, Qingfang Wan, Shan Chen, Qingqing Feng, Shujun You, Jiali Wang, Wei Zhu, Yimin Reprod Biol Endocrinol Research BACKGROUND: Unexplained recurrent spontaneous abortion (URSA) is one of the most challenging conditions frustrates women of childbearing age profoundly. The gene expression patterns and biological characteristics of placental villus in patients with URSA remain largely unknown. The aim of our study was to identify potential lncRNAs as well as their action mechanisms in URSA. METHOD: The ceRNA microarray was used to identify the mRNA and lncRNA expression profiles of URSA patients and normal pregnancy. Functional enrichment analyses for differentially expressed mRNAs in URSA were performed. Protein-protein interaction analysis of differentially expressed mRNAs was performed to identify hub genes and key modules. Subsequently, the co-dysregulated ceRNA network of URSA was established, and the enrichment analyses for the mRNAs in the ceRNA network was implemented. qRT-PCR was performed to validated the expression of key ENST00000429019 and mRNAs in URSA. RESULTS: We found that URSA placental villus have distinct mRNA and lncRNA expression profiles through ceRNA microarray, with a total of 347 mRNAs and 361 lncRNAs differentially expressed compared with controls. The functional enrichment analysis revealed that ncRNA processing, DNA replication, cell cycle, apoptosis, cytokine-mediated signaling pathway, ECM-receptor interaction were the potentially disrupted pathways in URSA patients. Then we constructed a co-dysregulated ceRNA network and found differentially expressed mRNAs were regulated by a small fraction of hub lncRNAs. Finally, we found a key network of ENST00000429019 and three cell proliferation or apoptosis related key mRNAs (CDCA3, KIFC1, NCAPH), and validated their expression and regulation in tissue and cellular levels. CONCLUSIONS: This study identified a key ceRNA network, which might take part in URSA and correlate with cell proliferation and apoptosis. Optimistically, this study may deepen our apprehensions about the underlying molecular and biological causes of URSA and provide an important theoretical basis for future therapeutic strategies for patients with URSA. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12958-023-01107-4. BioMed Central 2023-06-20 /pmc/articles/PMC10280933/ /pubmed/37340405 http://dx.doi.org/10.1186/s12958-023-01107-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Tang, Minyue
Li, Qingfang
Wan, Shan
Chen, Qingqing
Feng, Shujun
You, Jiali
Wang, Wei
Zhu, Yimin
LncRNA landscape and associated ceRNA network in placental villus of unexplained recurrent spontaneous abortion
title LncRNA landscape and associated ceRNA network in placental villus of unexplained recurrent spontaneous abortion
title_full LncRNA landscape and associated ceRNA network in placental villus of unexplained recurrent spontaneous abortion
title_fullStr LncRNA landscape and associated ceRNA network in placental villus of unexplained recurrent spontaneous abortion
title_full_unstemmed LncRNA landscape and associated ceRNA network in placental villus of unexplained recurrent spontaneous abortion
title_short LncRNA landscape and associated ceRNA network in placental villus of unexplained recurrent spontaneous abortion
title_sort lncrna landscape and associated cerna network in placental villus of unexplained recurrent spontaneous abortion
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10280933/
https://www.ncbi.nlm.nih.gov/pubmed/37340405
http://dx.doi.org/10.1186/s12958-023-01107-4
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