Cargando…

Assessment of the effects of hydrogen water on human gingival fibroblast cell culture in patients with chronic periodontitis

BACKGROUND: Activated inflammatory cells produce reactive oxygen species (ROS) to eliminate pathogens. Under normal conditions, the pathogens are taken care of, and tissues are repaired. However, in periodontal disease, persistent inflammation causes increased ROS release and impaired healing. There...

Descripción completa

Detalles Bibliográficos
Autores principales: Bhatt, Akanksha, Nayak, Aarati, Bhat, Kishore, Bogar, Chetana, Nayak, Ranganath, Naik, Sachita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10281311/
https://www.ncbi.nlm.nih.gov/pubmed/37346858
http://dx.doi.org/10.4103/jisp.jisp_546_21
_version_ 1785060983018881024
author Bhatt, Akanksha
Nayak, Aarati
Bhat, Kishore
Bogar, Chetana
Nayak, Ranganath
Naik, Sachita
author_facet Bhatt, Akanksha
Nayak, Aarati
Bhat, Kishore
Bogar, Chetana
Nayak, Ranganath
Naik, Sachita
author_sort Bhatt, Akanksha
collection PubMed
description BACKGROUND: Activated inflammatory cells produce reactive oxygen species (ROS) to eliminate pathogens. Under normal conditions, the pathogens are taken care of, and tissues are repaired. However, in periodontal disease, persistent inflammation causes increased ROS release and impaired healing. Therefore, removal of overproduced ROS using antioxidants is necessary. Hydrogen water has an antioxidative effect on cells and impedes oxidative stress-related disorders. AIM: To study the effect of hydrogen water on cell viability, migration, and its antioxidative potential in fibroblasts obtained from chronic periodontitis patients. MATERIALS AND METHODS: The gingival tissue samples were obtained from 26 subjects (13 periodontally healthy individuals and 13 chronic periodontitis patients) and processed. The human gingival fibroblasts were cultured and the assays were commenced once adequate growth was detected. The effect of hydrogen water on cell viability was checked by neutral red assay, while the migration potential was assessed by transwell migration assay. The antioxidative potential of hydrogen water was evaluated by CUPRAC assay. STATISTICAL ANALYSIS: Intergroup comparison was done using Mann–Whitney U-test. Intragroup comparison was done using Wilcoxon signed-rank test. RESULTS: Hydrogen water was nontoxic to the fibroblasts at 24 h and 48 h. The intergroup comparison of the cell viability between hydrogen water-treated periodontally healthy gingival fibroblasts (HF) and fibroblasts from patients with chronic periodontitis (CF) showed a statistically significant (P = 0.00) difference at 24 h and 48 h. Hydrogen water also positively influenced the migratory capacity. Hydrogen water-treated fibroblasts obtained from chronic periodontitis patients showed more migration in comparison to the healthy group (P = 0.00). Hydrogen water showed an antioxidative potential. The maximum potential was seen in relation to the fibroblasts obtained from chronic periodontitis patients at 48 h. CONCLUSION: Hydrogen water was nontoxic, increased the migratory capacity, and showed an antioxidative potential on human fibroblasts obtained from periodontally healthy individuals and patients with chronic periodontitis.
format Online
Article
Text
id pubmed-10281311
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Wolters Kluwer - Medknow
record_format MEDLINE/PubMed
spelling pubmed-102813112023-06-21 Assessment of the effects of hydrogen water on human gingival fibroblast cell culture in patients with chronic periodontitis Bhatt, Akanksha Nayak, Aarati Bhat, Kishore Bogar, Chetana Nayak, Ranganath Naik, Sachita J Indian Soc Periodontol Original Article BACKGROUND: Activated inflammatory cells produce reactive oxygen species (ROS) to eliminate pathogens. Under normal conditions, the pathogens are taken care of, and tissues are repaired. However, in periodontal disease, persistent inflammation causes increased ROS release and impaired healing. Therefore, removal of overproduced ROS using antioxidants is necessary. Hydrogen water has an antioxidative effect on cells and impedes oxidative stress-related disorders. AIM: To study the effect of hydrogen water on cell viability, migration, and its antioxidative potential in fibroblasts obtained from chronic periodontitis patients. MATERIALS AND METHODS: The gingival tissue samples were obtained from 26 subjects (13 periodontally healthy individuals and 13 chronic periodontitis patients) and processed. The human gingival fibroblasts were cultured and the assays were commenced once adequate growth was detected. The effect of hydrogen water on cell viability was checked by neutral red assay, while the migration potential was assessed by transwell migration assay. The antioxidative potential of hydrogen water was evaluated by CUPRAC assay. STATISTICAL ANALYSIS: Intergroup comparison was done using Mann–Whitney U-test. Intragroup comparison was done using Wilcoxon signed-rank test. RESULTS: Hydrogen water was nontoxic to the fibroblasts at 24 h and 48 h. The intergroup comparison of the cell viability between hydrogen water-treated periodontally healthy gingival fibroblasts (HF) and fibroblasts from patients with chronic periodontitis (CF) showed a statistically significant (P = 0.00) difference at 24 h and 48 h. Hydrogen water also positively influenced the migratory capacity. Hydrogen water-treated fibroblasts obtained from chronic periodontitis patients showed more migration in comparison to the healthy group (P = 0.00). Hydrogen water showed an antioxidative potential. The maximum potential was seen in relation to the fibroblasts obtained from chronic periodontitis patients at 48 h. CONCLUSION: Hydrogen water was nontoxic, increased the migratory capacity, and showed an antioxidative potential on human fibroblasts obtained from periodontally healthy individuals and patients with chronic periodontitis. Wolters Kluwer - Medknow 2023 2023-05-01 /pmc/articles/PMC10281311/ /pubmed/37346858 http://dx.doi.org/10.4103/jisp.jisp_546_21 Text en Copyright: © 2023 Indian Society of Periodontology https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Bhatt, Akanksha
Nayak, Aarati
Bhat, Kishore
Bogar, Chetana
Nayak, Ranganath
Naik, Sachita
Assessment of the effects of hydrogen water on human gingival fibroblast cell culture in patients with chronic periodontitis
title Assessment of the effects of hydrogen water on human gingival fibroblast cell culture in patients with chronic periodontitis
title_full Assessment of the effects of hydrogen water on human gingival fibroblast cell culture in patients with chronic periodontitis
title_fullStr Assessment of the effects of hydrogen water on human gingival fibroblast cell culture in patients with chronic periodontitis
title_full_unstemmed Assessment of the effects of hydrogen water on human gingival fibroblast cell culture in patients with chronic periodontitis
title_short Assessment of the effects of hydrogen water on human gingival fibroblast cell culture in patients with chronic periodontitis
title_sort assessment of the effects of hydrogen water on human gingival fibroblast cell culture in patients with chronic periodontitis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10281311/
https://www.ncbi.nlm.nih.gov/pubmed/37346858
http://dx.doi.org/10.4103/jisp.jisp_546_21
work_keys_str_mv AT bhattakanksha assessmentoftheeffectsofhydrogenwateronhumangingivalfibroblastcellcultureinpatientswithchronicperiodontitis
AT nayakaarati assessmentoftheeffectsofhydrogenwateronhumangingivalfibroblastcellcultureinpatientswithchronicperiodontitis
AT bhatkishore assessmentoftheeffectsofhydrogenwateronhumangingivalfibroblastcellcultureinpatientswithchronicperiodontitis
AT bogarchetana assessmentoftheeffectsofhydrogenwateronhumangingivalfibroblastcellcultureinpatientswithchronicperiodontitis
AT nayakranganath assessmentoftheeffectsofhydrogenwateronhumangingivalfibroblastcellcultureinpatientswithchronicperiodontitis
AT naiksachita assessmentoftheeffectsofhydrogenwateronhumangingivalfibroblastcellcultureinpatientswithchronicperiodontitis