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Poly (A)-specific ribonuclease deficiency impacts oogenesis in zebrafish
Poly (A)-specific ribonuclease (PARN) is the most important 3′–5′exonuclease involved in the process of deadenylation, the removal of poly (A) tails of mRNAs. Although PARN is primarily known for its role in mRNA stability, recent studies suggest several other functions of PARN including a role in t...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10281955/ https://www.ncbi.nlm.nih.gov/pubmed/37340076 http://dx.doi.org/10.1038/s41598-023-37226-6 |
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author | Nanjappa, Dechamma Pandyanda De Saffel, Hanna Kalladka, Krithika Arjuna, Srividya Babu, Nishith Prasad, Kishan Sips, Patrick Chakraborty, Anirban |
author_facet | Nanjappa, Dechamma Pandyanda De Saffel, Hanna Kalladka, Krithika Arjuna, Srividya Babu, Nishith Prasad, Kishan Sips, Patrick Chakraborty, Anirban |
author_sort | Nanjappa, Dechamma Pandyanda |
collection | PubMed |
description | Poly (A)-specific ribonuclease (PARN) is the most important 3′–5′exonuclease involved in the process of deadenylation, the removal of poly (A) tails of mRNAs. Although PARN is primarily known for its role in mRNA stability, recent studies suggest several other functions of PARN including a role in telomere biology, non-coding RNA maturation, trimming of miRNAs, ribosome biogenesis and TP53 function. Moreover, PARN expression is de-regulated in many cancers, including solid tumours and hematopoietic malignancies. To better understand the in vivo role of PARN, we used a zebrafish model to study the physiological consequences of Parn loss-of-function. Exon 19 of the gene, which partially codes for the RNA binding domain of the protein, was targeted for CRISPR-Cas9-directed genome editing. Contrary to the expectations, no developmental defects were observed in the zebrafish with a parn nonsense mutation. Intriguingly, the parn null mutants were viable and fertile, but turned out to only develop into males. Histological analysis of the gonads in the mutants and their wild type siblings revealed a defective maturation of gonadal cells in the parn null mutants. The results of this study highlight yet another emerging function of Parn, i.e., its role in oogenesis. |
format | Online Article Text |
id | pubmed-10281955 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-102819552023-06-22 Poly (A)-specific ribonuclease deficiency impacts oogenesis in zebrafish Nanjappa, Dechamma Pandyanda De Saffel, Hanna Kalladka, Krithika Arjuna, Srividya Babu, Nishith Prasad, Kishan Sips, Patrick Chakraborty, Anirban Sci Rep Article Poly (A)-specific ribonuclease (PARN) is the most important 3′–5′exonuclease involved in the process of deadenylation, the removal of poly (A) tails of mRNAs. Although PARN is primarily known for its role in mRNA stability, recent studies suggest several other functions of PARN including a role in telomere biology, non-coding RNA maturation, trimming of miRNAs, ribosome biogenesis and TP53 function. Moreover, PARN expression is de-regulated in many cancers, including solid tumours and hematopoietic malignancies. To better understand the in vivo role of PARN, we used a zebrafish model to study the physiological consequences of Parn loss-of-function. Exon 19 of the gene, which partially codes for the RNA binding domain of the protein, was targeted for CRISPR-Cas9-directed genome editing. Contrary to the expectations, no developmental defects were observed in the zebrafish with a parn nonsense mutation. Intriguingly, the parn null mutants were viable and fertile, but turned out to only develop into males. Histological analysis of the gonads in the mutants and their wild type siblings revealed a defective maturation of gonadal cells in the parn null mutants. The results of this study highlight yet another emerging function of Parn, i.e., its role in oogenesis. Nature Publishing Group UK 2023-06-20 /pmc/articles/PMC10281955/ /pubmed/37340076 http://dx.doi.org/10.1038/s41598-023-37226-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Nanjappa, Dechamma Pandyanda De Saffel, Hanna Kalladka, Krithika Arjuna, Srividya Babu, Nishith Prasad, Kishan Sips, Patrick Chakraborty, Anirban Poly (A)-specific ribonuclease deficiency impacts oogenesis in zebrafish |
title | Poly (A)-specific ribonuclease deficiency impacts oogenesis in zebrafish |
title_full | Poly (A)-specific ribonuclease deficiency impacts oogenesis in zebrafish |
title_fullStr | Poly (A)-specific ribonuclease deficiency impacts oogenesis in zebrafish |
title_full_unstemmed | Poly (A)-specific ribonuclease deficiency impacts oogenesis in zebrafish |
title_short | Poly (A)-specific ribonuclease deficiency impacts oogenesis in zebrafish |
title_sort | poly (a)-specific ribonuclease deficiency impacts oogenesis in zebrafish |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10281955/ https://www.ncbi.nlm.nih.gov/pubmed/37340076 http://dx.doi.org/10.1038/s41598-023-37226-6 |
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