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I329L protein-based indirect ELISA for detecting antibodies specific to African swine fever virus
African swine fever (ASF) is a disease that causes severe economic losses to the global porcine industry. As no vaccine or drug has been discovered for the prevention and control of ASF virus (ASFV), accurate diagnosis and timely eradication of infected animals are the primary measures, which necess...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10282770/ https://www.ncbi.nlm.nih.gov/pubmed/37351180 http://dx.doi.org/10.3389/fcimb.2023.1150042 |
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author | Shen, Zhiyong Qiu, Wenchen Luan, Haorui Sun, Chunxi Cao, Xinya Wang, Gang Peng, Jun |
author_facet | Shen, Zhiyong Qiu, Wenchen Luan, Haorui Sun, Chunxi Cao, Xinya Wang, Gang Peng, Jun |
author_sort | Shen, Zhiyong |
collection | PubMed |
description | African swine fever (ASF) is a disease that causes severe economic losses to the global porcine industry. As no vaccine or drug has been discovered for the prevention and control of ASF virus (ASFV), accurate diagnosis and timely eradication of infected animals are the primary measures, which necessitate accurate and effective detection methods. In this study, the truncated ASFV I329L (amino acids 70–237), was induced using IPTG and expressed in Escherichia coli cells. The highly antigenic viral protein I329L was used to develop an indirect enzyme-linked immunosorbent assay (iELISA), named I329L-ELISA, which cut-off value was 0.384. I329L-ELISA was used to detect 186 clinical pig serum samples, and the coincidence rate between the indirect ELISA developed here and the commercial kit was 96.77%. No cross-reactivity was observed with CSFV, PRRSV, PCV2, or PRV antibody-positive pig sera, indicating good specificity. Both intra- assay and inter-assay coefficients were below 10%, and the detection sensitivity of the iELISA reached 1:3200. In this study, an iELISA for ASFV antibody detection was developed based on the truncated ASFV I329L protein. Overall, the I329L-ELISA is a user-friendly detection tool that is suitable for ASFV antibody detection and epidemiological surveillance. |
format | Online Article Text |
id | pubmed-10282770 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102827702023-06-22 I329L protein-based indirect ELISA for detecting antibodies specific to African swine fever virus Shen, Zhiyong Qiu, Wenchen Luan, Haorui Sun, Chunxi Cao, Xinya Wang, Gang Peng, Jun Front Cell Infect Microbiol Cellular and Infection Microbiology African swine fever (ASF) is a disease that causes severe economic losses to the global porcine industry. As no vaccine or drug has been discovered for the prevention and control of ASF virus (ASFV), accurate diagnosis and timely eradication of infected animals are the primary measures, which necessitate accurate and effective detection methods. In this study, the truncated ASFV I329L (amino acids 70–237), was induced using IPTG and expressed in Escherichia coli cells. The highly antigenic viral protein I329L was used to develop an indirect enzyme-linked immunosorbent assay (iELISA), named I329L-ELISA, which cut-off value was 0.384. I329L-ELISA was used to detect 186 clinical pig serum samples, and the coincidence rate between the indirect ELISA developed here and the commercial kit was 96.77%. No cross-reactivity was observed with CSFV, PRRSV, PCV2, or PRV antibody-positive pig sera, indicating good specificity. Both intra- assay and inter-assay coefficients were below 10%, and the detection sensitivity of the iELISA reached 1:3200. In this study, an iELISA for ASFV antibody detection was developed based on the truncated ASFV I329L protein. Overall, the I329L-ELISA is a user-friendly detection tool that is suitable for ASFV antibody detection and epidemiological surveillance. Frontiers Media S.A. 2023-06-07 /pmc/articles/PMC10282770/ /pubmed/37351180 http://dx.doi.org/10.3389/fcimb.2023.1150042 Text en Copyright © 2023 Shen, Qiu, Luan, Sun, Cao, Wang and Peng https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Shen, Zhiyong Qiu, Wenchen Luan, Haorui Sun, Chunxi Cao, Xinya Wang, Gang Peng, Jun I329L protein-based indirect ELISA for detecting antibodies specific to African swine fever virus |
title | I329L protein-based indirect ELISA for detecting antibodies specific to African swine fever virus |
title_full | I329L protein-based indirect ELISA for detecting antibodies specific to African swine fever virus |
title_fullStr | I329L protein-based indirect ELISA for detecting antibodies specific to African swine fever virus |
title_full_unstemmed | I329L protein-based indirect ELISA for detecting antibodies specific to African swine fever virus |
title_short | I329L protein-based indirect ELISA for detecting antibodies specific to African swine fever virus |
title_sort | i329l protein-based indirect elisa for detecting antibodies specific to african swine fever virus |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10282770/ https://www.ncbi.nlm.nih.gov/pubmed/37351180 http://dx.doi.org/10.3389/fcimb.2023.1150042 |
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