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High resolution mapping of QTLs for fruit color and firmness in Amrapali/Sensation mango hybrids
INTRODUCTION: Mango (Mangifera indica L.), acclaimed as the ‘king of fruits’ in the tropical world, has historical, religious, and economic values. It is grown commercially in more than 100 countries, and fresh mango world trade accounts for ~3,200 million US dollars for the year 2020. Mango is wide...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10282835/ https://www.ncbi.nlm.nih.gov/pubmed/37351213 http://dx.doi.org/10.3389/fpls.2023.1135285 |
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author | Srivastav, Manish Radadiya, Nidhi Ramachandra, Sridhar Jayaswal, Pawan Kumar Singh, Nisha Singh, Sangeeta Mahato, Ajay Kumar Tandon, Gitanjali Gupta, Ankit Devi, Rajni Subrayagowda, Sreekanth Halli Kumar, Gulshan Prakash, Pragya Singh, Shivani Sharma, Nimisha Nagaraja, A. Kar, Abhijit Rudra, Shalini Gaur Sethi, Shruti Jaiswal, Sarika Iquebal, Mir Asif Singh, Rakesh Singh, Sanjay Kumar Singh, Nagendra Kumar |
author_facet | Srivastav, Manish Radadiya, Nidhi Ramachandra, Sridhar Jayaswal, Pawan Kumar Singh, Nisha Singh, Sangeeta Mahato, Ajay Kumar Tandon, Gitanjali Gupta, Ankit Devi, Rajni Subrayagowda, Sreekanth Halli Kumar, Gulshan Prakash, Pragya Singh, Shivani Sharma, Nimisha Nagaraja, A. Kar, Abhijit Rudra, Shalini Gaur Sethi, Shruti Jaiswal, Sarika Iquebal, Mir Asif Singh, Rakesh Singh, Sanjay Kumar Singh, Nagendra Kumar |
author_sort | Srivastav, Manish |
collection | PubMed |
description | INTRODUCTION: Mango (Mangifera indica L.), acclaimed as the ‘king of fruits’ in the tropical world, has historical, religious, and economic values. It is grown commercially in more than 100 countries, and fresh mango world trade accounts for ~3,200 million US dollars for the year 2020. Mango is widely cultivated in sub-tropical and tropical regions of the world, with India, China, and Thailand being the top three producers. Mango fruit is adored for its taste, color, flavor, and aroma. Fruit color and firmness are important fruit quality traits for consumer acceptance, but their genetics is poorly understood. METHODS: For mapping of fruit color and firmness, mango varieties Amrapali and Sensation, having contrasting fruit quality traits, were crossed for the development of a mapping population. Ninety-two bi-parental progenies obtained from this cross were used for the construction of a high-density linkage map and identification of QTLs. Genotyping was carried out using an 80K SNP chip array. RESULTS AND DISCUSSION: Initially, we constructed two high-density linkage maps based on the segregation of female and male parents. A female map with 3,213 SNPs and male map with 1,781 SNPs were distributed on 20 linkages groups covering map lengths of 2,844.39 and 2,684.22cM, respectively. Finally, the integrated map was constructed comprised of 4,361 SNP markers distributed on 20 linkage groups, which consisted of the chromosome haploid number in Mangifera indica (n =20). The integrated genetic map covered the entire genome of Mangifera indica cv. Dashehari, with a total genetic distance of 2,982.75 cM and an average distance between markers of 0.68 cM. The length of LGs varied from 85.78 to 218.28 cM, with a mean size of 149.14 cM. Phenotyping for fruit color and firmness traits was done for two consecutive seasons. We identified important consistent QTLs for 12 out of 20 traits, with integrated genetic linkages having significant LOD scores in at least one season. Important consistent QTLs for fruit peel color are located at Chr 3 and 18, and firmness on Chr 11 and 20. The QTLs mapped in this study would be useful in the marker-assisted breeding of mango for improved efficiency. |
format | Online Article Text |
id | pubmed-10282835 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102828352023-06-22 High resolution mapping of QTLs for fruit color and firmness in Amrapali/Sensation mango hybrids Srivastav, Manish Radadiya, Nidhi Ramachandra, Sridhar Jayaswal, Pawan Kumar Singh, Nisha Singh, Sangeeta Mahato, Ajay Kumar Tandon, Gitanjali Gupta, Ankit Devi, Rajni Subrayagowda, Sreekanth Halli Kumar, Gulshan Prakash, Pragya Singh, Shivani Sharma, Nimisha Nagaraja, A. Kar, Abhijit Rudra, Shalini Gaur Sethi, Shruti Jaiswal, Sarika Iquebal, Mir Asif Singh, Rakesh Singh, Sanjay Kumar Singh, Nagendra Kumar Front Plant Sci Plant Science INTRODUCTION: Mango (Mangifera indica L.), acclaimed as the ‘king of fruits’ in the tropical world, has historical, religious, and economic values. It is grown commercially in more than 100 countries, and fresh mango world trade accounts for ~3,200 million US dollars for the year 2020. Mango is widely cultivated in sub-tropical and tropical regions of the world, with India, China, and Thailand being the top three producers. Mango fruit is adored for its taste, color, flavor, and aroma. Fruit color and firmness are important fruit quality traits for consumer acceptance, but their genetics is poorly understood. METHODS: For mapping of fruit color and firmness, mango varieties Amrapali and Sensation, having contrasting fruit quality traits, were crossed for the development of a mapping population. Ninety-two bi-parental progenies obtained from this cross were used for the construction of a high-density linkage map and identification of QTLs. Genotyping was carried out using an 80K SNP chip array. RESULTS AND DISCUSSION: Initially, we constructed two high-density linkage maps based on the segregation of female and male parents. A female map with 3,213 SNPs and male map with 1,781 SNPs were distributed on 20 linkages groups covering map lengths of 2,844.39 and 2,684.22cM, respectively. Finally, the integrated map was constructed comprised of 4,361 SNP markers distributed on 20 linkage groups, which consisted of the chromosome haploid number in Mangifera indica (n =20). The integrated genetic map covered the entire genome of Mangifera indica cv. Dashehari, with a total genetic distance of 2,982.75 cM and an average distance between markers of 0.68 cM. The length of LGs varied from 85.78 to 218.28 cM, with a mean size of 149.14 cM. Phenotyping for fruit color and firmness traits was done for two consecutive seasons. We identified important consistent QTLs for 12 out of 20 traits, with integrated genetic linkages having significant LOD scores in at least one season. Important consistent QTLs for fruit peel color are located at Chr 3 and 18, and firmness on Chr 11 and 20. The QTLs mapped in this study would be useful in the marker-assisted breeding of mango for improved efficiency. Frontiers Media S.A. 2023-06-07 /pmc/articles/PMC10282835/ /pubmed/37351213 http://dx.doi.org/10.3389/fpls.2023.1135285 Text en Copyright © 2023 Srivastav, Radadiya, Ramachandra, Jayaswal, Singh, Singh, Mahato, Tandon, Gupta, Devi, Subrayagowda, Kumar, Prakash, Singh, Sharma, Nagaraja, Kar, Rudra, Sethi, Jaiswal, Iquebal, Singh, Singh and Singh https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Srivastav, Manish Radadiya, Nidhi Ramachandra, Sridhar Jayaswal, Pawan Kumar Singh, Nisha Singh, Sangeeta Mahato, Ajay Kumar Tandon, Gitanjali Gupta, Ankit Devi, Rajni Subrayagowda, Sreekanth Halli Kumar, Gulshan Prakash, Pragya Singh, Shivani Sharma, Nimisha Nagaraja, A. Kar, Abhijit Rudra, Shalini Gaur Sethi, Shruti Jaiswal, Sarika Iquebal, Mir Asif Singh, Rakesh Singh, Sanjay Kumar Singh, Nagendra Kumar High resolution mapping of QTLs for fruit color and firmness in Amrapali/Sensation mango hybrids |
title | High resolution mapping of QTLs for fruit color and firmness in Amrapali/Sensation mango hybrids |
title_full | High resolution mapping of QTLs for fruit color and firmness in Amrapali/Sensation mango hybrids |
title_fullStr | High resolution mapping of QTLs for fruit color and firmness in Amrapali/Sensation mango hybrids |
title_full_unstemmed | High resolution mapping of QTLs for fruit color and firmness in Amrapali/Sensation mango hybrids |
title_short | High resolution mapping of QTLs for fruit color and firmness in Amrapali/Sensation mango hybrids |
title_sort | high resolution mapping of qtls for fruit color and firmness in amrapali/sensation mango hybrids |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10282835/ https://www.ncbi.nlm.nih.gov/pubmed/37351213 http://dx.doi.org/10.3389/fpls.2023.1135285 |
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