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Chromosome-level genome assembly of Murraya paniculata sheds light on biosynthesis of floral volatiles

BACKGROUND: Murraya paniculata (L.) Jack, commonly called orange jessamine in the family Rutaceae, is an important ornamental plant in tropical and subtropical regions which is famous for its strong fragrance. Although genome assemblies have been reported for many Rutaceae species, mainly in the gen...

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Autores principales: Yang, Tianyu, Yin, Xin, Kang, Haotong, Yang, Danni, Yang, Xingyu, Yang, Yunqiang, Yang, Yongping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10283294/
https://www.ncbi.nlm.nih.gov/pubmed/37340448
http://dx.doi.org/10.1186/s12915-023-01639-6
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author Yang, Tianyu
Yin, Xin
Kang, Haotong
Yang, Danni
Yang, Xingyu
Yang, Yunqiang
Yang, Yongping
author_facet Yang, Tianyu
Yin, Xin
Kang, Haotong
Yang, Danni
Yang, Xingyu
Yang, Yunqiang
Yang, Yongping
author_sort Yang, Tianyu
collection PubMed
description BACKGROUND: Murraya paniculata (L.) Jack, commonly called orange jessamine in the family Rutaceae, is an important ornamental plant in tropical and subtropical regions which is famous for its strong fragrance. Although genome assemblies have been reported for many Rutaceae species, mainly in the genus Citrus, full genomic information has not been reported for M. paniculata, which is a prerequisite for in-depth genetic studies on Murraya and manipulation using genetic engineering techniques. Here, we report a high-quality chromosome-level genome assembly of M. paniculata and aim to provide insights on the molecular mechanisms of flower volatile biosynthesis. RESULTS: The genome assembly with a contig N50 of 18.25 Mb consists of 9 pseudomolecules and has a total length of 216.86 Mb. Phylogenetic analysis revealed that M. paniculata diverged from the common ancestor approximately 25 million years ago and has not undergone any species-specific whole genome duplication events. Genome structural annotation and comparative genomics analysis revealed that there are obvious differences in transposon contents among the genomes of M. paniculata and Citrus species, especially in the upstream regions of genes. Research on the flower volatiles of M. paniculata and C. maxima at three flowering stages revealed significant differences in volatile composition with the flowers of C. maxima lacking benzaldehyde and phenylacetaldehyde. Notably, there are transposons inserted in the upstream region of the phenylacetaldehyde synthase (PAAS) genes Cg1g029630 and Cg1g029640 in C. maxima, but not in the upstream region of three PAAS genes Me2G_2379, Me2G_2381, and Me2G_2382 in M. paniculata. Our results indicated that compared to the low expression levels of PAAS genes in C. maxima, the higher expression levels of the three PAAS genes in M. paniculata are the main factor affecting the phenylacetaldehyde biosynthesis and causing the content difference of phenylacetaldehyde. The phenylacetaldehyde synthetic activities of the enzymes encoded by M. paniculata PAAS genes were validated by in vitro analyses. CONCLUSIONS: Our study provides useful genomic resources of M. paniculata for further research on Rutaceae plants, identifies new PAAS genes, and provides insights into how transposons contribute to variations in flower volatiles among Murraya and Citrus plants. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12915-023-01639-6.
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spelling pubmed-102832942023-06-22 Chromosome-level genome assembly of Murraya paniculata sheds light on biosynthesis of floral volatiles Yang, Tianyu Yin, Xin Kang, Haotong Yang, Danni Yang, Xingyu Yang, Yunqiang Yang, Yongping BMC Biol Research Article BACKGROUND: Murraya paniculata (L.) Jack, commonly called orange jessamine in the family Rutaceae, is an important ornamental plant in tropical and subtropical regions which is famous for its strong fragrance. Although genome assemblies have been reported for many Rutaceae species, mainly in the genus Citrus, full genomic information has not been reported for M. paniculata, which is a prerequisite for in-depth genetic studies on Murraya and manipulation using genetic engineering techniques. Here, we report a high-quality chromosome-level genome assembly of M. paniculata and aim to provide insights on the molecular mechanisms of flower volatile biosynthesis. RESULTS: The genome assembly with a contig N50 of 18.25 Mb consists of 9 pseudomolecules and has a total length of 216.86 Mb. Phylogenetic analysis revealed that M. paniculata diverged from the common ancestor approximately 25 million years ago and has not undergone any species-specific whole genome duplication events. Genome structural annotation and comparative genomics analysis revealed that there are obvious differences in transposon contents among the genomes of M. paniculata and Citrus species, especially in the upstream regions of genes. Research on the flower volatiles of M. paniculata and C. maxima at three flowering stages revealed significant differences in volatile composition with the flowers of C. maxima lacking benzaldehyde and phenylacetaldehyde. Notably, there are transposons inserted in the upstream region of the phenylacetaldehyde synthase (PAAS) genes Cg1g029630 and Cg1g029640 in C. maxima, but not in the upstream region of three PAAS genes Me2G_2379, Me2G_2381, and Me2G_2382 in M. paniculata. Our results indicated that compared to the low expression levels of PAAS genes in C. maxima, the higher expression levels of the three PAAS genes in M. paniculata are the main factor affecting the phenylacetaldehyde biosynthesis and causing the content difference of phenylacetaldehyde. The phenylacetaldehyde synthetic activities of the enzymes encoded by M. paniculata PAAS genes were validated by in vitro analyses. CONCLUSIONS: Our study provides useful genomic resources of M. paniculata for further research on Rutaceae plants, identifies new PAAS genes, and provides insights into how transposons contribute to variations in flower volatiles among Murraya and Citrus plants. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12915-023-01639-6. BioMed Central 2023-06-20 /pmc/articles/PMC10283294/ /pubmed/37340448 http://dx.doi.org/10.1186/s12915-023-01639-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Yang, Tianyu
Yin, Xin
Kang, Haotong
Yang, Danni
Yang, Xingyu
Yang, Yunqiang
Yang, Yongping
Chromosome-level genome assembly of Murraya paniculata sheds light on biosynthesis of floral volatiles
title Chromosome-level genome assembly of Murraya paniculata sheds light on biosynthesis of floral volatiles
title_full Chromosome-level genome assembly of Murraya paniculata sheds light on biosynthesis of floral volatiles
title_fullStr Chromosome-level genome assembly of Murraya paniculata sheds light on biosynthesis of floral volatiles
title_full_unstemmed Chromosome-level genome assembly of Murraya paniculata sheds light on biosynthesis of floral volatiles
title_short Chromosome-level genome assembly of Murraya paniculata sheds light on biosynthesis of floral volatiles
title_sort chromosome-level genome assembly of murraya paniculata sheds light on biosynthesis of floral volatiles
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10283294/
https://www.ncbi.nlm.nih.gov/pubmed/37340448
http://dx.doi.org/10.1186/s12915-023-01639-6
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