Light-sheet autofluorescence lifetime imaging with a single-photon avalanche diode array

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SIGNIFICANCE: Fluorescence lifetime imaging microscopy (FLIM) of the metabolic co-enzyme nicotinamide adenine dinucleotide (phosphate) [NAD(P)H] is a popular method to monitor single-cell metabolism within unperturbed, living 3D systems. However, FLIM of NAD(P)H has not been performed in a light-she...

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Detalles Bibliográficos
Autores principales: Samimi, Kayvan, Desa, Danielle E., Lin, Wei, Weiss, Kurt, Li, Joe, Huisken, Jan, Miskolci, Veronika, Huttenlocher, Anna, Chacko, Jenu V., Velten, Andreas, Rogers, Jeremy D., Eliceiri, Kevin W., Skala, Melissa C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society of Photo-Optical Instrumentation Engineers 2023
Materias:
Microscopy
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10284079/
https://www.ncbi.nlm.nih.gov/pubmed/37351197
http://dx.doi.org/10.1117/1.JBO.28.6.066502
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