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Protective Effect of Vitamin D Supplementation on Some Hemogram Derived Inflammatory Indices in Normal and High-Fat Diet Fed Male Wistar Rats

BACKGROUND: Hematological inflammatory indices are currently suggested to assess systemic inflammation. This study aims to investigate a vitamin D supplementation effect on hematological indices of inflammation in rats. METHOD: Forty-eight middle-aged male rats were allocated into a normal diet (ND)...

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Autores principales: Agh, Fahimeh, Mousavi, Seyed H., Aryaeian, Naheed, Amiri, Fatemehsadat, Jalilvand, Mohammad R., Janani, Leila, Hasani, Motahareh, Sepahvand, Fatemeh, Zamani-Garmsiri, Fahimeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10284218/
https://www.ncbi.nlm.nih.gov/pubmed/37351039
http://dx.doi.org/10.4103/ijpvm.ijpvm_505_20
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author Agh, Fahimeh
Mousavi, Seyed H.
Aryaeian, Naheed
Amiri, Fatemehsadat
Jalilvand, Mohammad R.
Janani, Leila
Hasani, Motahareh
Sepahvand, Fatemeh
Zamani-Garmsiri, Fahimeh
author_facet Agh, Fahimeh
Mousavi, Seyed H.
Aryaeian, Naheed
Amiri, Fatemehsadat
Jalilvand, Mohammad R.
Janani, Leila
Hasani, Motahareh
Sepahvand, Fatemeh
Zamani-Garmsiri, Fahimeh
author_sort Agh, Fahimeh
collection PubMed
description BACKGROUND: Hematological inflammatory indices are currently suggested to assess systemic inflammation. This study aims to investigate a vitamin D supplementation effect on hematological indices of inflammation in rats. METHOD: Forty-eight middle-aged male rats were allocated into a normal diet (ND) group (10% fat) and a high-fat diet (HFD) group (60% fat). The animals were fed for 26 weeks. After this period, each group was randomly divided into three subgroups, each of 8 rats: Group (1): animals were fed the ND and HFD containing 1 IU/g vitamin D for 4 months, group (2): animals were fed the ND and HFD containing 6 IU/g vitamin D for 4 months and group (3): animals were euthanized to evaluate the HFD effect. Serum 25-hydroxyvitamin D level, white blood cell count (WBCs), platelet count, platelet crit (PCT), mean platelet volume (MPV), platelet distribution width (PDW), platelet-to-lymphocyte ratio (PLR), neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio (MLR) were measured. RESULTS: The HFD, significantly increased body weight, PCT, PDW, PLR, NLR, and MLR and significantly reduced serum vitamin D levels compared to the ND (P < 0.05). There was a significant decrease in food intake, MPV, PDW, and NLR after vitamin D supplementation in the ND-fed group (P < 0.05). A significant reduction in platelet count, PCT, and MLR was observed after vitamin D supplementation in HFD-fed rats (P < 0.05). CONCLUSIONS: In our study, some hemogram-derived inflammatory indices were higher in the HFD-fed group, and vitamin D supplementation lowering effects on some hematological indices were seen in both ND and HFD groups.
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spelling pubmed-102842182023-06-22 Protective Effect of Vitamin D Supplementation on Some Hemogram Derived Inflammatory Indices in Normal and High-Fat Diet Fed Male Wistar Rats Agh, Fahimeh Mousavi, Seyed H. Aryaeian, Naheed Amiri, Fatemehsadat Jalilvand, Mohammad R. Janani, Leila Hasani, Motahareh Sepahvand, Fatemeh Zamani-Garmsiri, Fahimeh Int J Prev Med Original Article BACKGROUND: Hematological inflammatory indices are currently suggested to assess systemic inflammation. This study aims to investigate a vitamin D supplementation effect on hematological indices of inflammation in rats. METHOD: Forty-eight middle-aged male rats were allocated into a normal diet (ND) group (10% fat) and a high-fat diet (HFD) group (60% fat). The animals were fed for 26 weeks. After this period, each group was randomly divided into three subgroups, each of 8 rats: Group (1): animals were fed the ND and HFD containing 1 IU/g vitamin D for 4 months, group (2): animals were fed the ND and HFD containing 6 IU/g vitamin D for 4 months and group (3): animals were euthanized to evaluate the HFD effect. Serum 25-hydroxyvitamin D level, white blood cell count (WBCs), platelet count, platelet crit (PCT), mean platelet volume (MPV), platelet distribution width (PDW), platelet-to-lymphocyte ratio (PLR), neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio (MLR) were measured. RESULTS: The HFD, significantly increased body weight, PCT, PDW, PLR, NLR, and MLR and significantly reduced serum vitamin D levels compared to the ND (P < 0.05). There was a significant decrease in food intake, MPV, PDW, and NLR after vitamin D supplementation in the ND-fed group (P < 0.05). A significant reduction in platelet count, PCT, and MLR was observed after vitamin D supplementation in HFD-fed rats (P < 0.05). CONCLUSIONS: In our study, some hemogram-derived inflammatory indices were higher in the HFD-fed group, and vitamin D supplementation lowering effects on some hematological indices were seen in both ND and HFD groups. Wolters Kluwer - Medknow 2023-04-26 /pmc/articles/PMC10284218/ /pubmed/37351039 http://dx.doi.org/10.4103/ijpvm.ijpvm_505_20 Text en Copyright: © 2023 International Journal of Preventive Medicine https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Agh, Fahimeh
Mousavi, Seyed H.
Aryaeian, Naheed
Amiri, Fatemehsadat
Jalilvand, Mohammad R.
Janani, Leila
Hasani, Motahareh
Sepahvand, Fatemeh
Zamani-Garmsiri, Fahimeh
Protective Effect of Vitamin D Supplementation on Some Hemogram Derived Inflammatory Indices in Normal and High-Fat Diet Fed Male Wistar Rats
title Protective Effect of Vitamin D Supplementation on Some Hemogram Derived Inflammatory Indices in Normal and High-Fat Diet Fed Male Wistar Rats
title_full Protective Effect of Vitamin D Supplementation on Some Hemogram Derived Inflammatory Indices in Normal and High-Fat Diet Fed Male Wistar Rats
title_fullStr Protective Effect of Vitamin D Supplementation on Some Hemogram Derived Inflammatory Indices in Normal and High-Fat Diet Fed Male Wistar Rats
title_full_unstemmed Protective Effect of Vitamin D Supplementation on Some Hemogram Derived Inflammatory Indices in Normal and High-Fat Diet Fed Male Wistar Rats
title_short Protective Effect of Vitamin D Supplementation on Some Hemogram Derived Inflammatory Indices in Normal and High-Fat Diet Fed Male Wistar Rats
title_sort protective effect of vitamin d supplementation on some hemogram derived inflammatory indices in normal and high-fat diet fed male wistar rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10284218/
https://www.ncbi.nlm.nih.gov/pubmed/37351039
http://dx.doi.org/10.4103/ijpvm.ijpvm_505_20
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