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Development of a candidate multi-epitope vaccine against Sphingobacterium spiritivorum : Reverse vaccinology and immunoinformatics approach
OBJECTIVES: To develop a candidate vaccine aginst the Sphingobacterium spiritivorum. METHODS: Since there is currently no vaccine against this pathogen, we employed in-silico methods to extensively explore the outer membrane toxin-producing proteins found specifically in S. spiritivorum to forecast...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Saudi Medical Journal
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10284220/ https://www.ncbi.nlm.nih.gov/pubmed/37343981 http://dx.doi.org/10.15537/smj.2023.44.6.20220733 |
Sumario: | OBJECTIVES: To develop a candidate vaccine aginst the Sphingobacterium spiritivorum. METHODS: Since there is currently no vaccine against this pathogen, we employed in-silico methods to extensively explore the outer membrane toxin-producing proteins found specifically in S. spiritivorum to forecast a multi-epitope chimeric vaccine design. This computational study was conducted in Saudi Arabia in 2022 (study design: computational; ethical approval not applicable). RESULTS: TThe vaccine peptide comprises multiple linear and conformational B-cell epitopes, which have the potential to elicit humoral immunity. Projected B-cell- derived T-cell epitopes for outer membrane proteins are present in the produced protein. The docking and molecular dynamic simulation results indicating that the chimeric vaccine had adequate binding stability with TLR-4. Following the immunological simulation, significant levels of immune cell expression were observed as immunoglobulin (Ig) M and IgG, IgM, IgM1, and IgM2, and independently IgG1 and IgG2. CONCLUSION: The developed vaccine candidate is suitable for further testing and can assist experimental vaccinologists in developing an effective vaccine against S. spiritivorum. |
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