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Characterization of cerebrospinal fluid (CSF) microbiota at the time of initial surgical intervention for children with hydrocephalus

OBJECTIVE: To characterize the microbiota of the cerebrospinal fluid (CSF) from children with hydrocephalus at the time of initial surgical intervention. STUDY DESIGN: CSF was obtained at initial surgical intervention. One aliquot was stored in skim milk-tryptone-glucose-glycerol (STGG) medium and t...

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Autores principales: Pandey, Shailly, Whitlock, Kathryn B., Test, Matthew R., Hodor, Paul, Pope, Christopher E., Limbrick, David D., McDonald, Patrick J., Hauptman, Jason S., Hoffman, Lucas R., Simon, Tamara D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10284395/
https://www.ncbi.nlm.nih.gov/pubmed/37342995
http://dx.doi.org/10.1371/journal.pone.0280682
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author Pandey, Shailly
Whitlock, Kathryn B.
Test, Matthew R.
Hodor, Paul
Pope, Christopher E.
Limbrick, David D.
McDonald, Patrick J.
Hauptman, Jason S.
Hoffman, Lucas R.
Simon, Tamara D.
author_facet Pandey, Shailly
Whitlock, Kathryn B.
Test, Matthew R.
Hodor, Paul
Pope, Christopher E.
Limbrick, David D.
McDonald, Patrick J.
Hauptman, Jason S.
Hoffman, Lucas R.
Simon, Tamara D.
author_sort Pandey, Shailly
collection PubMed
description OBJECTIVE: To characterize the microbiota of the cerebrospinal fluid (CSF) from children with hydrocephalus at the time of initial surgical intervention. STUDY DESIGN: CSF was obtained at initial surgical intervention. One aliquot was stored in skim milk-tryptone-glucose-glycerol (STGG) medium and the second was unprocessed; both were then stored at –70°C. Bacterial growth for CSF samples stored in STGG were subsequently characterized using aerobic and anaerobic culture on blood agar and MALDI-TOF sequencing. All unprocessed CSF samples underwent 16S quantitative polymerase chain reaction (qPCR) sequencing, and a subset underwent standard clinical microbiological culture. CSF with culture growth (either after storage in STGG or standard clinical) were further analyzed using whole-genome amplification sequencing (WGAS). RESULTS: 11/66 (17%) samples stored in STGG and 1/36 (3%) that underwent standard clinical microbiological culture demonstrated bacterial growth. Of the organisms present, 8 were common skin flora and 4 were potential pathogens; only 1 was also qPCR positive. WGAS findings and STGG culture findings were concordant for only 1 sample, identifying Staphylococcus epidermidis. No significant difference in time to second surgical intervention was observed between the STGG culture-positive and negative groups. CONCLUSION(S): Using high sensitivity methods, we detected the presence of bacteria in a subset of CSF samples at the time of first surgery. Therefore, the true presence of bacteria in CSF of children with hydrocephalus cannot be ruled out, though our findings may suggest these bacteria are contaminants or false positives of the detection methods. Regardless of origin, the detection of microbiota in the CSF of these children may not have any clinical significance.
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spelling pubmed-102843952023-06-22 Characterization of cerebrospinal fluid (CSF) microbiota at the time of initial surgical intervention for children with hydrocephalus Pandey, Shailly Whitlock, Kathryn B. Test, Matthew R. Hodor, Paul Pope, Christopher E. Limbrick, David D. McDonald, Patrick J. Hauptman, Jason S. Hoffman, Lucas R. Simon, Tamara D. PLoS One Research Article OBJECTIVE: To characterize the microbiota of the cerebrospinal fluid (CSF) from children with hydrocephalus at the time of initial surgical intervention. STUDY DESIGN: CSF was obtained at initial surgical intervention. One aliquot was stored in skim milk-tryptone-glucose-glycerol (STGG) medium and the second was unprocessed; both were then stored at –70°C. Bacterial growth for CSF samples stored in STGG were subsequently characterized using aerobic and anaerobic culture on blood agar and MALDI-TOF sequencing. All unprocessed CSF samples underwent 16S quantitative polymerase chain reaction (qPCR) sequencing, and a subset underwent standard clinical microbiological culture. CSF with culture growth (either after storage in STGG or standard clinical) were further analyzed using whole-genome amplification sequencing (WGAS). RESULTS: 11/66 (17%) samples stored in STGG and 1/36 (3%) that underwent standard clinical microbiological culture demonstrated bacterial growth. Of the organisms present, 8 were common skin flora and 4 were potential pathogens; only 1 was also qPCR positive. WGAS findings and STGG culture findings were concordant for only 1 sample, identifying Staphylococcus epidermidis. No significant difference in time to second surgical intervention was observed between the STGG culture-positive and negative groups. CONCLUSION(S): Using high sensitivity methods, we detected the presence of bacteria in a subset of CSF samples at the time of first surgery. Therefore, the true presence of bacteria in CSF of children with hydrocephalus cannot be ruled out, though our findings may suggest these bacteria are contaminants or false positives of the detection methods. Regardless of origin, the detection of microbiota in the CSF of these children may not have any clinical significance. Public Library of Science 2023-06-21 /pmc/articles/PMC10284395/ /pubmed/37342995 http://dx.doi.org/10.1371/journal.pone.0280682 Text en © 2023 Pandey et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Pandey, Shailly
Whitlock, Kathryn B.
Test, Matthew R.
Hodor, Paul
Pope, Christopher E.
Limbrick, David D.
McDonald, Patrick J.
Hauptman, Jason S.
Hoffman, Lucas R.
Simon, Tamara D.
Characterization of cerebrospinal fluid (CSF) microbiota at the time of initial surgical intervention for children with hydrocephalus
title Characterization of cerebrospinal fluid (CSF) microbiota at the time of initial surgical intervention for children with hydrocephalus
title_full Characterization of cerebrospinal fluid (CSF) microbiota at the time of initial surgical intervention for children with hydrocephalus
title_fullStr Characterization of cerebrospinal fluid (CSF) microbiota at the time of initial surgical intervention for children with hydrocephalus
title_full_unstemmed Characterization of cerebrospinal fluid (CSF) microbiota at the time of initial surgical intervention for children with hydrocephalus
title_short Characterization of cerebrospinal fluid (CSF) microbiota at the time of initial surgical intervention for children with hydrocephalus
title_sort characterization of cerebrospinal fluid (csf) microbiota at the time of initial surgical intervention for children with hydrocephalus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10284395/
https://www.ncbi.nlm.nih.gov/pubmed/37342995
http://dx.doi.org/10.1371/journal.pone.0280682
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