Cargando…

Acentrosomal spindles assemble from branching microtubule nucleation near chromosomes in Xenopus laevis egg extract

Microtubules are generated at centrosomes, chromosomes, and within spindles during cell division. Whereas microtubule nucleation at the centrosome is well characterized, much remains unknown about where, when, and how microtubules are nucleated at chromosomes. To address these questions, we reconsti...

Descripción completa

Detalles Bibliográficos
Autores principales:	Gouveia, Bernardo, Setru, Sagar U., King, Matthew R., Hamlin, Aaron, Stone, Howard A., Shaevitz, Joshua W., Petry, Sabine
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10284841/ https://www.ncbi.nlm.nih.gov/pubmed/37344488 http://dx.doi.org/10.1038/s41467-023-39041-z

Ejemplares similares

A hydrodynamic instability drives protein droplet formation on microtubules to nucleate branches
por: Setru, Sagar U., et al.
Publicado: (2021)

Autocatalytic microtubule nucleation determines the size and mass of Xenopus laevis egg extract spindles
por: Decker, Franziska, et al.
Publicado: (2018)

The kinesin Eg5 drives poleward microtubule flux in Xenopus laevis egg extract spindles
por: Miyamoto, David T., et al.
Publicado: (2004)

Ran-dependent TPX2 activation promotes acentrosomal microtubule nucleation in neurons
por: Chen, Wen-Shin, et al.
Publicado: (2017)

Biochemical reconstitution of branching microtubule nucleation
por: Alfaro-Aco, Raymundo, et al.
Publicado: (2020)

Poleward transport of Eg5 by dynein–dynactin in Xenopus laevis egg extract spindles
por: Uteng, Marianne, et al.
Publicado: (2008)

Spatiotemporal organization of branched microtubule networks
por: Thawani, Akanksha, et al.
Publicado: (2019)

Obtaining Eggs from Xenopus laevis Females
por: K. Cross, Marie, et al.
Publicado: (2008)

Preparation and Fractionation of Xenopus laevis Egg Extracts
por: K. Cross, Marie, et al.
Publicado: (2008)

Meiosis-Specific Stable Binding of Augmin to Acentrosomal Spindle Poles Promotes Biased Microtubule Assembly in Oocytes
por: Colombié, Nathalie, et al.
Publicado: (2013)

Structural basis of protein condensation on microtubules underlying branching microtubule nucleation
por: Guo, Changmiao, et al.
Publicado: (2023)

Structural analysis of the role of TPX2 in branching microtubule nucleation
por: Alfaro-Aco, Raymundo, et al.
Publicado: (2017)

XCTK2: A Kinesin-related Protein That Promotes Mitotic Spindle Assembly in Xenopus laevis Egg Extracts
por: Walczak, Claire E., et al.
Publicado: (1997)

High-quality frozen extracts of Xenopus laevis eggs reveal size-dependent control of metaphase spindle micromechanics
por: Takagi, Jun, et al.
Publicado: (2017)

Xenopus laevis lectin is localized at several sites in Xenopus oocytes, eggs, and embryos
Publicado: (1983)

Augmin-dependent microtubule nucleation at microtubule walls in the spindle
por: Kamasaki, Tomoko, et al.
Publicado: (2013)

Spindle Assembly in Xenopus Egg Extracts: Respective Roles of Centrosomes and Microtubule Self-Organization
por: Heald, Rebecca, et al.
Publicado: (1997)

Self-organization of stabilized microtubules by both spindle and midzone mechanisms in Xenopus egg cytosol
por: Mitchison, Timothy J., et al.
Publicado: (2013)

Two Kinesin-14A Motors Oligomerize to Drive Poleward Microtubule Convergence for Acentrosomal Spindle Morphogenesis in Arabidopsis thaliana
por: Hotta, Takashi, et al.
Publicado: (2022)

Acentrosomal spindle assembly and maintenance in Caenorhabditis elegans oocytes requires a kinesin-12 nonmotor microtubule interaction domain
por: Wolff, Ian D., et al.
Publicado: (2022)

Microtubule-dependent pushing forces contribute to long-distance aster movement and centration in Xenopus laevis egg extracts
por: Sulerud, Taylor, et al.
Publicado: (2020)

Thrombopoietin induces production of nucleated thrombocytes from liver cells in Xenopus laevis
por: Tanizaki, Yuta, et al.
Publicado: (2015)

Twin Xenopus laevis embryos appearing from flattened eggs
por: SATO, Eiji
Publicado: (2014)

ZYG-9(ch-TOG) promotes the stability of acentrosomal poles via regulation of spindle microtubules in C. elegans oocyte meiosis
por: Cavin-Meza, Gabriel, et al.
Publicado: (2022)

Microglia reactivity entails microtubule remodeling from acentrosomal to centrosomal arrays
por: Rosito, Maria, et al.
Publicado: (2023)

Assembly of Caenorhabditis elegans acentrosomal spindles occurs without evident microtubule-organizing centers and requires microtubule sorting by KLP-18/kinesin-12 and MESP-1
por: Wolff, Ian D., et al.
Publicado: (2016)

The transition state and regulation of γ-TuRC-mediated microtubule nucleation revealed by single molecule microscopy
por: Thawani, Akanksha, et al.
Publicado: (2020)

Cortex reorganization of Xenopus laevis eggs in strong static magnetic fields
por: Mietchen, Daniel, et al.
Publicado: (2005)

Aster formation in eggs of Xenopus laevis. Induction by isolated basal bodies
Publicado: (1975)

Observations on intracellular pH during cleavage of eggs of Xenopus laevis
Publicado: (1981)

In vitro reconstitution of branching microtubule nucleation
por: Tariq, Ammarah, et al.
Publicado: (2020)

Regulation of the microtubule nucleating activity of centrosomes in Xenopus egg extracts: role of cyclin A-associated protein kinase
Publicado: (1992)

Phosphorylation by Cdk1 Increases the Binding of Eg5 to Microtubules In Vitro and in Xenopus Egg Extract Spindles
por: Cahu, Julie, et al.
Publicado: (2008)

Self-organization of an acentrosomal microtubule network at the basal cortex of polarized epithelial cells
por: Reilein, Amy, et al.
Publicado: (2005)

Lateral microtubule bundles promote chromosome alignment during acentrosomal oocyte meiosis
por: Wignall, Sarah M., et al.
Publicado: (2009)

Msps governs acentrosomal microtubule assembly and reactivation of quiescent neural stem cells
por: Deng, Qiannan, et al.
Publicado: (2021)

Spindle-to-cortex communication in cleaving, polyspermic Xenopus eggs
por: Field, Christine M., et al.
Publicado: (2015)

Subcellular Metabolite and Lipid Analysis of Xenopus laevis Eggs by LAESI Mass Spectrometry
por: Shrestha, Bindesh, et al.
Publicado: (2014)

Studying chromosome biology with single-molecule resolution in Xenopus laevis egg extracts
por: Cameron, George, et al.
Publicado: (2021)

Direct observation of microtubule dynamics at kinetochores in Xenopus extract spindles: implications for spindle mechanics
por: Maddox, Paul, et al.
Publicado: (2003)

Cannot write session to /tmp/vufind_sessions/sess_ok6cijis4jq494gi49rqihk23s