Synthesis and photodynamic antimicrobial chemotherapy against multi-drug resistant Proteus mirabilis of ornithine-porphyrin conjugates in vitro and in vivo

For the treatment of bacterial infections, photodynamic antimicrobial chemotherapy (PACT) has the advantage of circumventing multi-drug resistance. In this work, new cationic photosensitizers against multi-drug resistant Proteus mirabilis (MRPM) were designed and synthesized by the conjugation of am...

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Autores principales: Meng, Shuai, Xu, Zengping, Wang, Xueming, Liu, Yang, Li, Bole, Zhang, Jie, Zhang, Xiaolong, Liu, Tianjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10285166/
https://www.ncbi.nlm.nih.gov/pubmed/37362917
http://dx.doi.org/10.3389/fmicb.2023.1196072
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author Meng, Shuai
Xu, Zengping
Wang, Xueming
Liu, Yang
Li, Bole
Zhang, Jie
Zhang, Xiaolong
Liu, Tianjun
author_facet Meng, Shuai
Xu, Zengping
Wang, Xueming
Liu, Yang
Li, Bole
Zhang, Jie
Zhang, Xiaolong
Liu, Tianjun
author_sort Meng, Shuai
collection PubMed
description For the treatment of bacterial infections, photodynamic antimicrobial chemotherapy (PACT) has the advantage of circumventing multi-drug resistance. In this work, new cationic photosensitizers against multi-drug resistant Proteus mirabilis (MRPM) were designed and synthesized by the conjugation of amino phenyl porphyrin with basic amino acid L-ornithine. Their photoinactivation efficacies against MRPM in vitro were reported and include the influence of laser energy, uptake, MIC and MBC, dose-dependent photoinactivation effects, membrane integrity, and fluorescence imaging. The PACT in vivo was evaluated using a wound mouse model infected by MRPM. Photosensitizer 4d displayed high photo inactivation efficacy against MRPM at 7.81 μM under illumination, and it could accelerate wound healing via bactericidal effect. These ornithine-porphyrin conjugates are potential photosensitizers for PACT in the treatment of MRPM infection.
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spelling pubmed-102851662023-06-23 Synthesis and photodynamic antimicrobial chemotherapy against multi-drug resistant Proteus mirabilis of ornithine-porphyrin conjugates in vitro and in vivo Meng, Shuai Xu, Zengping Wang, Xueming Liu, Yang Li, Bole Zhang, Jie Zhang, Xiaolong Liu, Tianjun Front Microbiol Microbiology For the treatment of bacterial infections, photodynamic antimicrobial chemotherapy (PACT) has the advantage of circumventing multi-drug resistance. In this work, new cationic photosensitizers against multi-drug resistant Proteus mirabilis (MRPM) were designed and synthesized by the conjugation of amino phenyl porphyrin with basic amino acid L-ornithine. Their photoinactivation efficacies against MRPM in vitro were reported and include the influence of laser energy, uptake, MIC and MBC, dose-dependent photoinactivation effects, membrane integrity, and fluorescence imaging. The PACT in vivo was evaluated using a wound mouse model infected by MRPM. Photosensitizer 4d displayed high photo inactivation efficacy against MRPM at 7.81 μM under illumination, and it could accelerate wound healing via bactericidal effect. These ornithine-porphyrin conjugates are potential photosensitizers for PACT in the treatment of MRPM infection. Frontiers Media S.A. 2023-06-08 /pmc/articles/PMC10285166/ /pubmed/37362917 http://dx.doi.org/10.3389/fmicb.2023.1196072 Text en Copyright © 2023 Meng, Xu, Wang, Liu, Li, Zhang, Zhang and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Meng, Shuai
Xu, Zengping
Wang, Xueming
Liu, Yang
Li, Bole
Zhang, Jie
Zhang, Xiaolong
Liu, Tianjun
Synthesis and photodynamic antimicrobial chemotherapy against multi-drug resistant Proteus mirabilis of ornithine-porphyrin conjugates in vitro and in vivo
title Synthesis and photodynamic antimicrobial chemotherapy against multi-drug resistant Proteus mirabilis of ornithine-porphyrin conjugates in vitro and in vivo
title_full Synthesis and photodynamic antimicrobial chemotherapy against multi-drug resistant Proteus mirabilis of ornithine-porphyrin conjugates in vitro and in vivo
title_fullStr Synthesis and photodynamic antimicrobial chemotherapy against multi-drug resistant Proteus mirabilis of ornithine-porphyrin conjugates in vitro and in vivo
title_full_unstemmed Synthesis and photodynamic antimicrobial chemotherapy against multi-drug resistant Proteus mirabilis of ornithine-porphyrin conjugates in vitro and in vivo
title_short Synthesis and photodynamic antimicrobial chemotherapy against multi-drug resistant Proteus mirabilis of ornithine-porphyrin conjugates in vitro and in vivo
title_sort synthesis and photodynamic antimicrobial chemotherapy against multi-drug resistant proteus mirabilis of ornithine-porphyrin conjugates in vitro and in vivo
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10285166/
https://www.ncbi.nlm.nih.gov/pubmed/37362917
http://dx.doi.org/10.3389/fmicb.2023.1196072
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