The functional and regulatory entities underlying free and peptide-bound amino acid transporters in the bovine mammary gland

Free and peptide-bound AA act as building blocks and key regulators of milk protein. To improve milk protein production, mammary epithelial cells of lactating mammals require extensive AA movement across the plasma membrane via multiple transport systems. Recent studies on bovine mammary cells/tissues have expanded the number of AA transporter systems identified and the knowledge on their contribution to AA utilization for milk protein synthesis and the regulatory machinery. However, in lactating cows, the exact intracellular location of mammary AA transporters and the extent of mammary net AA utilization for milk protein production remain unclear. This review highlights the existing knowledge on various characteristics, such as substrate specificity, kinetics, their effects on AA uptake and utilization, and regulatory mechanism, of recently examined bovine mammary free and peptide-bound AA transporters.