The identification of high-preforming antibodies for Ubiquilin-2 for use in Western Blot, immunoprecipitation, and immunofluorescence

Ubiquilin-2, a member of the ubiquilin protein family, plays a role in the regulation of various protein degradation pathways, and is mutated in some neurodegenerative diseases. Well-characterized anti-Ubiquilin-2 antibodies would advance reproducible research for Ubiquilin-2 and in turn, benefit th...

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Autores principales: McDowell, Ian, Ayoubi, Riham, Fotouhi, Maryam, Southern, Kathleen, McPherson, Peter S., Laflamme, Carl
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000 Research Limited 2023
Materias:
Data Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10285353/
https://www.ncbi.nlm.nih.gov/pubmed/37359784
http://dx.doi.org/10.12688/f1000research.131851.1
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author McDowell, Ian
Ayoubi, Riham
Fotouhi, Maryam
Southern, Kathleen
McPherson, Peter S.
Laflamme, Carl
author_facet McDowell, Ian
Ayoubi, Riham
Fotouhi, Maryam
Southern, Kathleen
McPherson, Peter S.
Laflamme, Carl
author_sort McDowell, Ian
collection PubMed
description Ubiquilin-2, a member of the ubiquilin protein family, plays a role in the regulation of various protein degradation pathways, and is mutated in some neurodegenerative diseases. Well-characterized anti-Ubiquilin-2 antibodies would advance reproducible research for Ubiquilin-2 and in turn, benefit the scientific community. In this study, we characterized ten Ubiquilin-2 commercial antibodies for Western Blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. We identified many high-performing antibodies and encourage readers to use this report as a guide to select the most appropriate antibody for their specific needs.
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spelling pubmed-102853532023-06-23 The identification of high-preforming antibodies for Ubiquilin-2 for use in Western Blot, immunoprecipitation, and immunofluorescence McDowell, Ian Ayoubi, Riham Fotouhi, Maryam Southern, Kathleen McPherson, Peter S. Laflamme, Carl F1000Res Data Note Ubiquilin-2, a member of the ubiquilin protein family, plays a role in the regulation of various protein degradation pathways, and is mutated in some neurodegenerative diseases. Well-characterized anti-Ubiquilin-2 antibodies would advance reproducible research for Ubiquilin-2 and in turn, benefit the scientific community. In this study, we characterized ten Ubiquilin-2 commercial antibodies for Western Blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. We identified many high-performing antibodies and encourage readers to use this report as a guide to select the most appropriate antibody for their specific needs. F1000 Research Limited 2023-03-30 /pmc/articles/PMC10285353/ /pubmed/37359784 http://dx.doi.org/10.12688/f1000research.131851.1 Text en Copyright: © 2023 McDowell I et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Data Note
McDowell, Ian
Ayoubi, Riham
Fotouhi, Maryam
Southern, Kathleen
McPherson, Peter S.
Laflamme, Carl
The identification of high-preforming antibodies for Ubiquilin-2 for use in Western Blot, immunoprecipitation, and immunofluorescence
title The identification of high-preforming antibodies for Ubiquilin-2 for use in Western Blot, immunoprecipitation, and immunofluorescence
title_full The identification of high-preforming antibodies for Ubiquilin-2 for use in Western Blot, immunoprecipitation, and immunofluorescence
title_fullStr The identification of high-preforming antibodies for Ubiquilin-2 for use in Western Blot, immunoprecipitation, and immunofluorescence
title_full_unstemmed The identification of high-preforming antibodies for Ubiquilin-2 for use in Western Blot, immunoprecipitation, and immunofluorescence
title_short The identification of high-preforming antibodies for Ubiquilin-2 for use in Western Blot, immunoprecipitation, and immunofluorescence
title_sort identification of high-preforming antibodies for ubiquilin-2 for use in western blot, immunoprecipitation, and immunofluorescence
topic Data Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10285353/
https://www.ncbi.nlm.nih.gov/pubmed/37359784
http://dx.doi.org/10.12688/f1000research.131851.1
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