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Quantitation of Residual Host Cell DNA in Recombinant Adeno-Associated Virus Using Droplet Digital Polymerase Chain Reaction

Recombinant adeno-associated virus (rAAV) is a viral vector commonly used in gene therapy. Residual host cell DNA is an impurity that has been associated with the risk of infection and oncogenicity. Thus, it needs to be monitored for quality control. We aimed to develop a droplet digital polymerase...

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Detalles Bibliográficos
Autores principales:	Higashiyama, Kiyoko, Yuan, Yuzhe, Hashiba, Noriko, Masumi-Koizumi, Kyoko, Yusa, Keisuke, Uchida, Kazuhisa
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Mary Ann Liebert, Inc., publishers 2023
Materias:	Methods
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10285681/ https://www.ncbi.nlm.nih.gov/pubmed/37058356 http://dx.doi.org/10.1089/hum.2023.006

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