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Large-scale single-cell cloning of genome-edited cultured human cells by On-chip SPiS
Single-cell cloning is the simplest strategy to isolate genome-edited cell clones, although its scalability has been an issue. Here, we present a protocol to establish genome-edited human cultured cell clones using the On-chip SPiS, a single-cell auto-dispensing device with image recognition technol...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10285693/ https://www.ncbi.nlm.nih.gov/pubmed/37329509 http://dx.doi.org/10.1016/j.xpro.2023.102364 |