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Genetic mapping and identification of Rht8-B1 that regulates plant height in wheat
BACKGROUND: Plant height (PH) and spike compactness (SC) are important agronomic traits that affect yield improvement in wheat crops. The identification of the loci or genes responsible for these traits is thus of great importance for marker-assisted selection in wheat breeding. RESULTS: In this stu...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10286341/ https://www.ncbi.nlm.nih.gov/pubmed/37349717 http://dx.doi.org/10.1186/s12870-023-04343-3 |
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author | Zhou, Chunyun Xiong, Hongchun Fu, Meiyu Guo, Huijun Zhao, Linshu Xie, Yongdun Gu, Jiayu Zhao, Shirong Ding, Yuping Li, Yuting Li, Xuejun Liu, Luxiang |
author_facet | Zhou, Chunyun Xiong, Hongchun Fu, Meiyu Guo, Huijun Zhao, Linshu Xie, Yongdun Gu, Jiayu Zhao, Shirong Ding, Yuping Li, Yuting Li, Xuejun Liu, Luxiang |
author_sort | Zhou, Chunyun |
collection | PubMed |
description | BACKGROUND: Plant height (PH) and spike compactness (SC) are important agronomic traits that affect yield improvement in wheat crops. The identification of the loci or genes responsible for these traits is thus of great importance for marker-assisted selection in wheat breeding. RESULTS: In this study, we used a recombinant inbred line (RIL) population with 139 lines derived from a cross between the mutant Rht8-2 and the local wheat variety NongDa5181 (ND5181) to construct a high-density genetic linkage map by applying the Wheat 40 K Panel. We identified seven stable QTLs for PH (three) and SC (four) in two environments using the RIL population, and found that Rht8-B1 is the causal gene of qPH2B.1 by further genetic mapping, gene cloning and gene editing analyses. Our results also showed that two natural variants from GC to TT in the coding region of Rht8-B1 resulted in an amino acid change from G (ND5181) to V (Rht8-2) at the 175(th) position, reducing PH by 3.6%~6.2% in the RIL population. Moreover, gene editing analysis suggested that the height of T(2) generation in Rht8-B1 edited plants was reduced by 5.6%, and that the impact of Rht8-B1 on PH was significantly lower than Rht8-D1. Additionally, analysis of the distribution of Rht8-B1 in various wheat resources suggested that the Rht8-B1b allele has not been widely utilized in modern wheat breeding. CONCLUSIONS: The combination of Rht8-B1b with other favorable Rht genes might be an alternative approach for developing lodging-resistant crops. Our study provides important information for marker-assisted selection in wheat breeding. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04343-3. |
format | Online Article Text |
id | pubmed-10286341 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-102863412023-06-23 Genetic mapping and identification of Rht8-B1 that regulates plant height in wheat Zhou, Chunyun Xiong, Hongchun Fu, Meiyu Guo, Huijun Zhao, Linshu Xie, Yongdun Gu, Jiayu Zhao, Shirong Ding, Yuping Li, Yuting Li, Xuejun Liu, Luxiang BMC Plant Biol Research BACKGROUND: Plant height (PH) and spike compactness (SC) are important agronomic traits that affect yield improvement in wheat crops. The identification of the loci or genes responsible for these traits is thus of great importance for marker-assisted selection in wheat breeding. RESULTS: In this study, we used a recombinant inbred line (RIL) population with 139 lines derived from a cross between the mutant Rht8-2 and the local wheat variety NongDa5181 (ND5181) to construct a high-density genetic linkage map by applying the Wheat 40 K Panel. We identified seven stable QTLs for PH (three) and SC (four) in two environments using the RIL population, and found that Rht8-B1 is the causal gene of qPH2B.1 by further genetic mapping, gene cloning and gene editing analyses. Our results also showed that two natural variants from GC to TT in the coding region of Rht8-B1 resulted in an amino acid change from G (ND5181) to V (Rht8-2) at the 175(th) position, reducing PH by 3.6%~6.2% in the RIL population. Moreover, gene editing analysis suggested that the height of T(2) generation in Rht8-B1 edited plants was reduced by 5.6%, and that the impact of Rht8-B1 on PH was significantly lower than Rht8-D1. Additionally, analysis of the distribution of Rht8-B1 in various wheat resources suggested that the Rht8-B1b allele has not been widely utilized in modern wheat breeding. CONCLUSIONS: The combination of Rht8-B1b with other favorable Rht genes might be an alternative approach for developing lodging-resistant crops. Our study provides important information for marker-assisted selection in wheat breeding. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04343-3. BioMed Central 2023-06-22 /pmc/articles/PMC10286341/ /pubmed/37349717 http://dx.doi.org/10.1186/s12870-023-04343-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Zhou, Chunyun Xiong, Hongchun Fu, Meiyu Guo, Huijun Zhao, Linshu Xie, Yongdun Gu, Jiayu Zhao, Shirong Ding, Yuping Li, Yuting Li, Xuejun Liu, Luxiang Genetic mapping and identification of Rht8-B1 that regulates plant height in wheat |
title | Genetic mapping and identification of Rht8-B1 that regulates plant height in wheat |
title_full | Genetic mapping and identification of Rht8-B1 that regulates plant height in wheat |
title_fullStr | Genetic mapping and identification of Rht8-B1 that regulates plant height in wheat |
title_full_unstemmed | Genetic mapping and identification of Rht8-B1 that regulates plant height in wheat |
title_short | Genetic mapping and identification of Rht8-B1 that regulates plant height in wheat |
title_sort | genetic mapping and identification of rht8-b1 that regulates plant height in wheat |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10286341/ https://www.ncbi.nlm.nih.gov/pubmed/37349717 http://dx.doi.org/10.1186/s12870-023-04343-3 |
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