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Accelerated Polyethylene Terephthalate (PET) Enzymatic Degradation by Room Temperature Alkali Pre‐treatment for Reduced Polymer Crystallinity

Polyethylene terephthalate (PET) is the most widely employed plastic for single‐use applications. The use of enzymes isolated from microorganisms, such as PETase with the capacity to hydrolyze PET into its monomers, represents a promising method for its sustainable recycling. However, the accessibil...

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Autores principales: Giraldo‐Narcizo, Sariah, Guenani, Nihal, Sánchez‐Pérez, Ana María, Guerrero, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10286761/
https://www.ncbi.nlm.nih.gov/pubmed/36341520
http://dx.doi.org/10.1002/cbic.202200503
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author Giraldo‐Narcizo, Sariah
Guenani, Nihal
Sánchez‐Pérez, Ana María
Guerrero, Antonio
author_facet Giraldo‐Narcizo, Sariah
Guenani, Nihal
Sánchez‐Pérez, Ana María
Guerrero, Antonio
author_sort Giraldo‐Narcizo, Sariah
collection PubMed
description Polyethylene terephthalate (PET) is the most widely employed plastic for single‐use applications. The use of enzymes isolated from microorganisms, such as PETase with the capacity to hydrolyze PET into its monomers, represents a promising method for its sustainable recycling. However, the accessibility of the enzyme to the hydrolysable bonds is an important challenge that needs to be addressed for effective biodegradation of postconsumer PET. Here, we combined an alkali pre‐treatment (25 °C) with PETase incubation (30 °C) with post‐consumed PET bottles. The pre‐treatment modifies the surface of the plastic and decreases its crystallinity enabling the access of the enzyme to the hydrolysable chemical bonds. When the alkali pre‐treatment is incorporated into the enzymatic process the degradation yields increase more than one order of magnitude reaching values comparable to those obtained during heating/cooling cycles. Our results show energetic advantages over other reported pre‐treatments and open new avenues for sustainable PET recycling.
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spelling pubmed-102867612023-06-23 Accelerated Polyethylene Terephthalate (PET) Enzymatic Degradation by Room Temperature Alkali Pre‐treatment for Reduced Polymer Crystallinity Giraldo‐Narcizo, Sariah Guenani, Nihal Sánchez‐Pérez, Ana María Guerrero, Antonio Chembiochem Research Articles Polyethylene terephthalate (PET) is the most widely employed plastic for single‐use applications. The use of enzymes isolated from microorganisms, such as PETase with the capacity to hydrolyze PET into its monomers, represents a promising method for its sustainable recycling. However, the accessibility of the enzyme to the hydrolysable bonds is an important challenge that needs to be addressed for effective biodegradation of postconsumer PET. Here, we combined an alkali pre‐treatment (25 °C) with PETase incubation (30 °C) with post‐consumed PET bottles. The pre‐treatment modifies the surface of the plastic and decreases its crystallinity enabling the access of the enzyme to the hydrolysable chemical bonds. When the alkali pre‐treatment is incorporated into the enzymatic process the degradation yields increase more than one order of magnitude reaching values comparable to those obtained during heating/cooling cycles. Our results show energetic advantages over other reported pre‐treatments and open new avenues for sustainable PET recycling. John Wiley and Sons Inc. 2022-11-30 2023-01-03 /pmc/articles/PMC10286761/ /pubmed/36341520 http://dx.doi.org/10.1002/cbic.202200503 Text en © 2022 The Authors. ChemBioChem published by Wiley-VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Giraldo‐Narcizo, Sariah
Guenani, Nihal
Sánchez‐Pérez, Ana María
Guerrero, Antonio
Accelerated Polyethylene Terephthalate (PET) Enzymatic Degradation by Room Temperature Alkali Pre‐treatment for Reduced Polymer Crystallinity
title Accelerated Polyethylene Terephthalate (PET) Enzymatic Degradation by Room Temperature Alkali Pre‐treatment for Reduced Polymer Crystallinity
title_full Accelerated Polyethylene Terephthalate (PET) Enzymatic Degradation by Room Temperature Alkali Pre‐treatment for Reduced Polymer Crystallinity
title_fullStr Accelerated Polyethylene Terephthalate (PET) Enzymatic Degradation by Room Temperature Alkali Pre‐treatment for Reduced Polymer Crystallinity
title_full_unstemmed Accelerated Polyethylene Terephthalate (PET) Enzymatic Degradation by Room Temperature Alkali Pre‐treatment for Reduced Polymer Crystallinity
title_short Accelerated Polyethylene Terephthalate (PET) Enzymatic Degradation by Room Temperature Alkali Pre‐treatment for Reduced Polymer Crystallinity
title_sort accelerated polyethylene terephthalate (pet) enzymatic degradation by room temperature alkali pre‐treatment for reduced polymer crystallinity
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10286761/
https://www.ncbi.nlm.nih.gov/pubmed/36341520
http://dx.doi.org/10.1002/cbic.202200503
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