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Position-dependent sequence motif preferences of SpCas9 are largely determined by scaffold-complementary spacer motifs

Streptococcus pyogenes Cas9 (SpCas9) nuclease exhibits considerable position-dependent sequence preferences. The reason behind these preferences is not well understood and is difficult to rationalise, since the protein establishes interactions with the target-spacer duplex in a sequence-independent...

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Detalles Bibliográficos
Autores principales:	Huszár, Krisztina, Welker, Zsombor, Györgypál, Zoltán, Tóth, Eszter, Ligeti, Zoltán, Kulcsár, Péter István, Dancsó, János, Tálas, András, Krausz, Sarah Laura, Varga, Éva, Welker, Ervin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2023
Materias:	RNA and RNA-protein complexes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10287927/ https://www.ncbi.nlm.nih.gov/pubmed/37140059 http://dx.doi.org/10.1093/nar/gkad323

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