Accurate quantification of DNA using on-site PCR (osPCR) by characterizing DNA amplification at single-molecule resolution

Despite the need in various applications, accurate quantification of nucleic acids still remains a challenge. The widely-used qPCR has reduced accuracy at ultralow template concentration and is susceptible to nonspecific amplifications. The more recently developed dPCR is costly and cannot handle hi...

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Detalles Bibliográficos
Autores principales: Ding, Ruihua, Liu, Liying, Zhang, Jiali, Lv, Pengxiao, Zhou, Lin, Zhang, Tinglu, Li, Shenwei, Zhao, Ran, Yang, Zhuo, Xiong, Peng, Chen, Hu, Wang, Wei, Wang, Hualiang, Tian, Zhengan, Liu, Bo, Chen, Chang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10287937/
https://www.ncbi.nlm.nih.gov/pubmed/37194709
http://dx.doi.org/10.1093/nar/gkad388

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