Cargando…

Absolute quantification of viable Vibrio cholerae in seawater samples using multiplex droplet digital PCR combined with propidium monoazide

INTRODUCTION: Toxigenic Vibrio cholerae serogroup O1 and O139 are the pathogens responsible for the global cholera epidemic. V. cholerae can settle in the water and spread via the fecal-oral route. Rapid and accurate monitoring of live V. cholerae in environmental water has become an important strat...

Descripción completa

Detalles Bibliográficos
Autores principales:	Yang, Jinsong, Xu, Haibin, Ke, Zili, Kan, Naipeng, Zheng, Enhui, Qiu, Yufeng, Huang, Mengying
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2023
Materias:	Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10288211/ https://www.ncbi.nlm.nih.gov/pubmed/37362935 http://dx.doi.org/10.3389/fmicb.2023.1149981

Ejemplares similares

Enumeration of Viable Non-Culturable Vibrio cholerae Using Droplet Digital PCR Combined With Propidium Monoazide Treatment
por: Zhao, Shuo, et al.
Publicado: (2021)

Experimental design for the optimization of propidium monoazide treatment to quantify viable and non-viable bacteria in piggery effluents
por: Desneux, Jérémy, et al.
Publicado: (2015)

Selective detection of viable seed-borne Acidovorax citrulli by real-time PCR with propidium monoazide
por: Tian, Qian, et al.
Publicado: (2016)

An Assay Combining Droplet Digital PCR With Propidium Monoazide Treatment for the Accurate Detection of Live Cells of Vibrio vulnificus in Plasma Samples
por: Hu, Ling, et al.
Publicado: (2022)

A Propidium Monoazide (PMAxx)-Droplet Digital PCR (ddPCR) for the Detection of Viable Burkholderia cepacia Complex in Nuclease-Free Water and Antiseptics
por: Daddy Gaoh, Soumana, et al.
Publicado: (2022)

Development of a propidium monoazide-polymerase chain reaction assay for detection of viable Lactobacillus brevis in beer
por: Ma, Yanlin, et al.
Publicado: (2017)

Evaluation of Propidium Monoazide Real-Time PCR for Early Detection of Viable Mycobacterium tuberculosis in Clinical Respiratory Specimens
por: Kim, Young Jin, et al.
Publicado: (2014)

Rapid Detection and Quantification of Viable Cells of Pectobacterium brasiliense Using Propidium Monoazide Combined with Real-Time PCR
por: Li, Junhui, et al.
Publicado: (2023)

Monitoring the prevalence of viable and dead cariogenic bacteria in oral specimens and in vitro biofilms by qPCR combined with propidium monoazide
por: Yasunaga, Ai, et al.
Publicado: (2013)

Quantitative Polymerase Chain Reaction Coupled With Sodium Dodecyl Sulfate and Propidium Monoazide for Detection of Viable Streptococcus agalactiae in Milk
por: Zhao, Yankun, et al.
Publicado: (2019)

A Novel Propidium Monoazide-Based PCR Assay Can Measure Viable Uropathogenic E. coli In Vitro and In Vivo
por: Lee, Albert S., et al.
Publicado: (2022)

An Improved Method for Quantification of Viable Fusarium Cells in Infected Soil Products by Propidium Monoazide Coupled with Real-Time PCR
por: Chen, Lida, et al.
Publicado: (2022)

Absolute Quantification of Viable but Nonculturable Vibrio cholerae Using Droplet Digital PCR with Oil-Enveloped Bacterial Cells
por: Zhao, Shuo, et al.
Publicado: (2022)

Detection of viable Salmonella in ice cream by TaqMan real-time polymerase chain reaction assay combining propidium monoazide
por: Wang, Yuexia, et al.
Publicado: (2015)

The effects of removing dead bacteria by propidium monoazide on the profile of salivary microbiome
por: Ren, Qidi, et al.
Publicado: (2021)

Designing primers and evaluation of the efficiency of propidium monoazide – Quantitative polymerase chain reaction for counting the viable cells of Lactobacillus gasseri and Lactobacillus salivarius
por: Lai, Chieh-Hsien, et al.
Publicado: (2016)

Applicability of propidium monoazide (PMA) for discrimination between living and dead phytoplankton cells
por: Joo, Sungbae, et al.
Publicado: (2019)

Whole microbial community viability is not quantitatively reflected by propidium monoazide sequencing approach
por: Wang, Ya, et al.
Publicado: (2021)

Detection of suid herpesvirus 1 infectivity in pigs by propidium monoazide-qPCR
por: Yang, Liu, et al.
Publicado: (2022)

Development and Application of a Simple “Easy To Operate” Propidium Monoazide-Crossing Priming Amplification on Detection of Viable and Viable But Non-culturable Cells of O157 Escherichia coli
por: Zhou, Wenqu, et al.
Publicado: (2020)

Advantageous Direct Quantification of Viable Closely Related Probiotics in Petit-Suisse Cheeses under In Vitro Gastrointestinal Conditions by Propidium Monoazide - qPCR
por: Villarreal, Martha Lissete Morales, et al.
Publicado: (2013)

Rapid Monitoring of Viable Genetically Modified Escherichia coli Using a Cell-Direct Quantitative PCR Method Combined with Propidium Monoazide Treatment
por: Qin, Yang, et al.
Publicado: (2023)

An Optimized Method to Assess Viable Escherichia coli O157:H7 in Agricultural Soil Using Combined Propidium Monoazide Staining and Quantitative PCR
por: Fu, Yulong, et al.
Publicado: (2020)

Detection of Viable but Non-Culturable (VBNC)-Campylobacter in the Environment of Broiler Farms: Innovative Insights Delivered by Propidium Monoazide (PMA)-v-qPCR Analysis
por: Reichelt, Benjamin, et al.
Publicado: (2023)

Peptoid Efficacy against Polymicrobial Biofilms Determined by Using Propidium Monoazide‐Modified Quantitative PCR
por: Luo, Yu, et al.
Publicado: (2016)

Determination of the viability of Toxoplasma gondii oocysts by PCR real-time after treatment with propidium monoazide
por: Marciano, Maria Aparecida Moraes, et al.
Publicado: (2020)

Propidium Monoazide Combined With RT-qPCR Detects Infectivity of Porcine Epidemic Diarrhea Virus
por: Liang, Gong, et al.
Publicado: (2022)

Point-of-Care Lateral Flow Detection of Viable Escherichia coli O157:H7 Using an Improved Propidium Monoazide-Recombinase Polymerase Amplification Method
por: Rani, Alka, et al.
Publicado: (2022)

Propidium monoazide (PMA) and ethidium bromide monoazide (EMA) improve DNA array and high-throughput sequencing of porcine reproductive and respiratory syndrome virus identification
por: Bellehumeur, Christian, et al.
Publicado: (2015)

Assessment of Probiotic Viability during Cheddar Cheese Manufacture and Ripening Using Propidium Monoazide-PCR Quantification
por: Desfossés-Foucault, Émilie, et al.
Publicado: (2012)

Limitations of Using Propidium Monoazide with qPCR to Discriminate between Live and Dead Legionella in Biofilm Samples
por: Taylor, Michael J, et al.
Publicado: (2014)

Effects of Propidium Monoazide (PMA) Treatment on Mycobiome and Bacteriome Analysis of Cystic Fibrosis Airways during Exacerbation
por: Nguyen, Linh Do Ngoc, et al.
Publicado: (2016)

Rapid molecular phenotypic antimicrobial susceptibility test for Neisseria gonorrhoeae based on propidium monoazide viability PCR
por: Tjandra, Kristel C., et al.
Publicado: (2023)

Prospects for the application of infectious virus detection technology based on propidium monoazide in African swine fever management
por: Zeng, Dexin, et al.
Publicado: (2022)

Detection of Viable and Total Bacterial Community in the Pit Mud of Chinese Strong-Flavor Liquor Using Propidium Monoazide Combined With Quantitative PCR and 16S rRNA Gene Sequencing
por: Tan, Guangxun, et al.
Publicado: (2020)

Method to quantify live and dead cells in multi-species oral biofilm by real-time PCR with propidium monoazide
por: Àlvarez, Gerard, et al.
Publicado: (2013)

Viability of Legionella pneumophila in Water Samples: A Comparison of Propidium Monoazide (PMA) Treatment on Membrane Filters and in Liquid
por: Bonetta, Sara, et al.
Publicado: (2017)

Persistence of Legionella in Routinely Disinfected Heater-Cooler Units and Heater Units assessed by Propidium Monoazide qPCR
por: Ditommaso, Savina, et al.
Publicado: (2020)

Two-Round Treatment With Propidium Monoazide Completely Inhibits the Detection of Dead Campylobacter spp. Cells by Quantitative PCR
por: Okada, Ayaka, et al.
Publicado: (2022)

Development of a Propidium Monoazide-Based Viability Quantitative PCR Assay for Red Sea Bream Iridovirus Detection
por: Kim, Kyung-Ho, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_s5t08v4u0k7vf3aoiut1mjsrjh