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Circulating DNA methylation level of CXCR5 correlates with inflammation in patients with rheumatoid arthritis

OBJECTIVES: To assess the differences in circulating DNA methylation levels of CXCR5 between rheumatoid arthritis (RA) and osteoarthritis (OA) and healthy controls (HC), and the correlation of methylation changes with clinical characteristics of RA patients. METHODS: Peripheral blood samples were co...

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Autores principales: Shi, Yiming, Chang, Cen, Xu, Lingxia, Jiang, Ping, Wei, Kai, Zhao, Jianan, Xu, Linshuai, Jin, Yehua, Zhang, Runrun, Wang, Huijuan, Qian, Yi, Qin, Yingying, Ding, Qin, Jiang, Ting, Guo, Shicheng, Wang, Rongsheng, He, Dongyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10288483/
https://www.ncbi.nlm.nih.gov/pubmed/37382265
http://dx.doi.org/10.1002/iid3.902
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author Shi, Yiming
Chang, Cen
Xu, Lingxia
Jiang, Ping
Wei, Kai
Zhao, Jianan
Xu, Linshuai
Jin, Yehua
Zhang, Runrun
Wang, Huijuan
Qian, Yi
Qin, Yingying
Ding, Qin
Jiang, Ting
Guo, Shicheng
Wang, Rongsheng
He, Dongyi
author_facet Shi, Yiming
Chang, Cen
Xu, Lingxia
Jiang, Ping
Wei, Kai
Zhao, Jianan
Xu, Linshuai
Jin, Yehua
Zhang, Runrun
Wang, Huijuan
Qian, Yi
Qin, Yingying
Ding, Qin
Jiang, Ting
Guo, Shicheng
Wang, Rongsheng
He, Dongyi
author_sort Shi, Yiming
collection PubMed
description OBJECTIVES: To assess the differences in circulating DNA methylation levels of CXCR5 between rheumatoid arthritis (RA) and osteoarthritis (OA) and healthy controls (HC), and the correlation of methylation changes with clinical characteristics of RA patients. METHODS: Peripheral blood samples were collected from 239 RA patients, 30 patients with OA, and 29 HC. Target region methylation sequencing to the promoter region of CXCR5 was achieved using MethylTarget. The methylation level of cg04537602 and methylation haplotype were compared among the three groups, and the correlation between methylation levels and clinical characteristics of RA patients was performed by Spearman's rank correlation analysis. RESULTS: The methylation level of cg04537602 was significantly higher in the peripheral blood of RA patients compared with OA patients (p = 1.3 × 10(−3)) and in the HC group (p = 5.5 × 10(−) (4)). The sensitivity was enhanced when CXCR5 methylation level combined with rheumatoid factor and anti–cyclic citrullinated peptide with area under curve (AUC) of 0.982 (95% confidence interval 0.970–0.995). The methylation level of cg04537602 in RA was positively correlated with C‐reactive protein (CRP) (r = .16, p = .01), and in RA patients aged 60 years and above, cg04537602 methylation levels were positively correlated with CRP (r = .31, p = 4.7 × 10(−) (4)), tender joint count (r = .21, p = .02), visual analog scales score (r = .21, p = .02), Disease Activity Score in 28 joints (DAS28) using the CRP level DAS28‐CRP (r = .27, p = 2.1 × 10(−) (3)), and DAS28‐ESR (r = .22, p = .01). We also observed significant differences of DNA methylation haplotypes in RA patients compared with OA patients and HC, which was consistent with single‐loci‐based CpG methylation measurement. CONCLUSION: The methylation level of CXCR5 was significantly higher in RA patients than in OA and HC, and correlated with the level of inflammation in RA patients, our study establishes a link between CXCR5 DNA methylation and clinical features that may help in the diagnosis and disease management of RA patients.
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spelling pubmed-102884832023-06-24 Circulating DNA methylation level of CXCR5 correlates with inflammation in patients with rheumatoid arthritis Shi, Yiming Chang, Cen Xu, Lingxia Jiang, Ping Wei, Kai Zhao, Jianan Xu, Linshuai Jin, Yehua Zhang, Runrun Wang, Huijuan Qian, Yi Qin, Yingying Ding, Qin Jiang, Ting Guo, Shicheng Wang, Rongsheng He, Dongyi Immun Inflamm Dis Original Articles OBJECTIVES: To assess the differences in circulating DNA methylation levels of CXCR5 between rheumatoid arthritis (RA) and osteoarthritis (OA) and healthy controls (HC), and the correlation of methylation changes with clinical characteristics of RA patients. METHODS: Peripheral blood samples were collected from 239 RA patients, 30 patients with OA, and 29 HC. Target region methylation sequencing to the promoter region of CXCR5 was achieved using MethylTarget. The methylation level of cg04537602 and methylation haplotype were compared among the three groups, and the correlation between methylation levels and clinical characteristics of RA patients was performed by Spearman's rank correlation analysis. RESULTS: The methylation level of cg04537602 was significantly higher in the peripheral blood of RA patients compared with OA patients (p = 1.3 × 10(−3)) and in the HC group (p = 5.5 × 10(−) (4)). The sensitivity was enhanced when CXCR5 methylation level combined with rheumatoid factor and anti–cyclic citrullinated peptide with area under curve (AUC) of 0.982 (95% confidence interval 0.970–0.995). The methylation level of cg04537602 in RA was positively correlated with C‐reactive protein (CRP) (r = .16, p = .01), and in RA patients aged 60 years and above, cg04537602 methylation levels were positively correlated with CRP (r = .31, p = 4.7 × 10(−) (4)), tender joint count (r = .21, p = .02), visual analog scales score (r = .21, p = .02), Disease Activity Score in 28 joints (DAS28) using the CRP level DAS28‐CRP (r = .27, p = 2.1 × 10(−) (3)), and DAS28‐ESR (r = .22, p = .01). We also observed significant differences of DNA methylation haplotypes in RA patients compared with OA patients and HC, which was consistent with single‐loci‐based CpG methylation measurement. CONCLUSION: The methylation level of CXCR5 was significantly higher in RA patients than in OA and HC, and correlated with the level of inflammation in RA patients, our study establishes a link between CXCR5 DNA methylation and clinical features that may help in the diagnosis and disease management of RA patients. John Wiley and Sons Inc. 2023-06-23 /pmc/articles/PMC10288483/ /pubmed/37382265 http://dx.doi.org/10.1002/iid3.902 Text en © 2023 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Shi, Yiming
Chang, Cen
Xu, Lingxia
Jiang, Ping
Wei, Kai
Zhao, Jianan
Xu, Linshuai
Jin, Yehua
Zhang, Runrun
Wang, Huijuan
Qian, Yi
Qin, Yingying
Ding, Qin
Jiang, Ting
Guo, Shicheng
Wang, Rongsheng
He, Dongyi
Circulating DNA methylation level of CXCR5 correlates with inflammation in patients with rheumatoid arthritis
title Circulating DNA methylation level of CXCR5 correlates with inflammation in patients with rheumatoid arthritis
title_full Circulating DNA methylation level of CXCR5 correlates with inflammation in patients with rheumatoid arthritis
title_fullStr Circulating DNA methylation level of CXCR5 correlates with inflammation in patients with rheumatoid arthritis
title_full_unstemmed Circulating DNA methylation level of CXCR5 correlates with inflammation in patients with rheumatoid arthritis
title_short Circulating DNA methylation level of CXCR5 correlates with inflammation in patients with rheumatoid arthritis
title_sort circulating dna methylation level of cxcr5 correlates with inflammation in patients with rheumatoid arthritis
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10288483/
https://www.ncbi.nlm.nih.gov/pubmed/37382265
http://dx.doi.org/10.1002/iid3.902
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