A disulfide chaperone knockout facilitates spin labeling and pulse EPR spectroscopy of outer membrane transporters

Pulse EPR measurements provide information on distances and distance distributions in proteins but require the incorporation of pairs of spin labels that are usually attached to engineered cysteine residues. In previous work, we demonstrated that efficient in vivo labeling of the Escherichia coli ou...

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Detalles Bibliográficos
Autores principales: Wimalasiri, Viranga W., Jurczak, Kinga A., Wieliniec, Monika K., Nilaweera, Thushani D., Nakamoto, Robert K., Cafiso, David S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2023
Materias:
Methods and Applications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10288552/
https://www.ncbi.nlm.nih.gov/pubmed/37312651
http://dx.doi.org/10.1002/pro.4704

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10288552/
https://www.ncbi.nlm.nih.gov/pubmed/37312651
http://dx.doi.org/10.1002/pro.4704

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