Cargando…
A disulfide chaperone knockout facilitates spin labeling and pulse EPR spectroscopy of outer membrane transporters
Pulse EPR measurements provide information on distances and distance distributions in proteins but require the incorporation of pairs of spin labels that are usually attached to engineered cysteine residues. In previous work, we demonstrated that efficient in vivo labeling of the Escherichia coli ou...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley & Sons, Inc.
2023
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10288552/ https://www.ncbi.nlm.nih.gov/pubmed/37312651 http://dx.doi.org/10.1002/pro.4704 |