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Differential expression of coagulation pathway-related proteins in diabetic urine exosomes
BACKGROUND: Coagulation function monitoring is important for the occurrence and development of diabetes. A total of 16 related proteins are involved in coagulation, but how these proteins change in diabetic urine exosomes is unclear. To explore the expression changes of coagulation-related proteins...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10288686/ https://www.ncbi.nlm.nih.gov/pubmed/37349729 http://dx.doi.org/10.1186/s12933-023-01887-4 |
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author | Liu, Tianci Liu, Na Wang, Yizhao Li, Tao Zhang, Man |
author_facet | Liu, Tianci Liu, Na Wang, Yizhao Li, Tao Zhang, Man |
author_sort | Liu, Tianci |
collection | PubMed |
description | BACKGROUND: Coagulation function monitoring is important for the occurrence and development of diabetes. A total of 16 related proteins are involved in coagulation, but how these proteins change in diabetic urine exosomes is unclear. To explore the expression changes of coagulation-related proteins in urine exosomes and their possible roles in the pathogenesis of diabetes, we performed proteomic analysis and finally applied them to the noninvasive monitoring of diabetes. METHODS: Subject urine samples were collected. LC-MS/MS was used to collect the information on coagulation-related proteins in urine exosomes. ELISA, mass spectrometry and western blotting were used to further verify the differential protein expression in urine exosomes. Correlations with clinical indicators were explored, and receiver operating characteristic (ROC) curves were drawn to evaluate the value of differential proteins in diabetes monitoring. RESULTS: Analyzing urine exosome proteomics data, eight coagulation-related proteins were found in this study. Among them, F2 was elevated in urine exosomes of diabetic patients compared with healthy controls. The results of ELISA, mass spectrometry and western blotting further verified the changes in F2. Correlation analysis showed that the expression of urine exosome F2 was correlated with clinical lipid metabolism indexes, and the concentration of F2 was strongly positively correlated with blood TG levels (P < 0.05). ROC curve analysis showed that F2 protein in urine exosomes had a good monitoring value for diabetes. CONCLUSION: Coagulation-related proteins were expressed in urine exosomes. Among them, F2 was increased in diabetic urine exosomes and may be a potential biomarker for monitoring diabetic changes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12933-023-01887-4. |
format | Online Article Text |
id | pubmed-10288686 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-102886862023-06-24 Differential expression of coagulation pathway-related proteins in diabetic urine exosomes Liu, Tianci Liu, Na Wang, Yizhao Li, Tao Zhang, Man Cardiovasc Diabetol Research BACKGROUND: Coagulation function monitoring is important for the occurrence and development of diabetes. A total of 16 related proteins are involved in coagulation, but how these proteins change in diabetic urine exosomes is unclear. To explore the expression changes of coagulation-related proteins in urine exosomes and their possible roles in the pathogenesis of diabetes, we performed proteomic analysis and finally applied them to the noninvasive monitoring of diabetes. METHODS: Subject urine samples were collected. LC-MS/MS was used to collect the information on coagulation-related proteins in urine exosomes. ELISA, mass spectrometry and western blotting were used to further verify the differential protein expression in urine exosomes. Correlations with clinical indicators were explored, and receiver operating characteristic (ROC) curves were drawn to evaluate the value of differential proteins in diabetes monitoring. RESULTS: Analyzing urine exosome proteomics data, eight coagulation-related proteins were found in this study. Among them, F2 was elevated in urine exosomes of diabetic patients compared with healthy controls. The results of ELISA, mass spectrometry and western blotting further verified the changes in F2. Correlation analysis showed that the expression of urine exosome F2 was correlated with clinical lipid metabolism indexes, and the concentration of F2 was strongly positively correlated with blood TG levels (P < 0.05). ROC curve analysis showed that F2 protein in urine exosomes had a good monitoring value for diabetes. CONCLUSION: Coagulation-related proteins were expressed in urine exosomes. Among them, F2 was increased in diabetic urine exosomes and may be a potential biomarker for monitoring diabetic changes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12933-023-01887-4. BioMed Central 2023-06-22 /pmc/articles/PMC10288686/ /pubmed/37349729 http://dx.doi.org/10.1186/s12933-023-01887-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Liu, Tianci Liu, Na Wang, Yizhao Li, Tao Zhang, Man Differential expression of coagulation pathway-related proteins in diabetic urine exosomes |
title | Differential expression of coagulation pathway-related proteins in diabetic urine exosomes |
title_full | Differential expression of coagulation pathway-related proteins in diabetic urine exosomes |
title_fullStr | Differential expression of coagulation pathway-related proteins in diabetic urine exosomes |
title_full_unstemmed | Differential expression of coagulation pathway-related proteins in diabetic urine exosomes |
title_short | Differential expression of coagulation pathway-related proteins in diabetic urine exosomes |
title_sort | differential expression of coagulation pathway-related proteins in diabetic urine exosomes |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10288686/ https://www.ncbi.nlm.nih.gov/pubmed/37349729 http://dx.doi.org/10.1186/s12933-023-01887-4 |
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