Cargando…
NMR spectroscopy-based analysis of gallstones of cancerous and benign gallbladders from different geographical regions of the Indian subcontinent
Analysis of the chemical composition of gallstones is vital for the etiopathogenesis of gallstone diseases that can ultimately help in the prevention of its formation. In the present study, gallstones from seven different regions of India were analyzed to highlight the major difference in their comp...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10289436/ https://www.ncbi.nlm.nih.gov/pubmed/37352214 http://dx.doi.org/10.1371/journal.pone.0286979 |
_version_ | 1785062278035406848 |
---|---|
author | Siddiqui, Mohd Adnan Dwivedi, Navneet Siddiqui, Mohammed Haris Rana, S. V. Sharma, Anil Dash, N. R. Pradeep, Rebala Vijayahari, Ranjit Behari, Anu Kapoor, V. K. Sinha, Neeraj |
author_facet | Siddiqui, Mohd Adnan Dwivedi, Navneet Siddiqui, Mohammed Haris Rana, S. V. Sharma, Anil Dash, N. R. Pradeep, Rebala Vijayahari, Ranjit Behari, Anu Kapoor, V. K. Sinha, Neeraj |
author_sort | Siddiqui, Mohd Adnan |
collection | PubMed |
description | Analysis of the chemical composition of gallstones is vital for the etiopathogenesis of gallstone diseases that can ultimately help in the prevention of its formation. In the present study, gallstones from seven different regions of India were analyzed to highlight the major difference in their composition. Also, gallstones of different pathological conditions i.e., benign (chronic cholecystitis, CC) and malignant gallbladder disease (gallbladder cancer GBC) were characterized. The type of polymorphs of cholesterol molecules was also studied to provide insight into the structure of gallstones. (1)H solution state NMR spectroscopy 1D experiments were performed on a total of 94 gallstone (GS) samples collected from seven different geographical regions of India. Solid-State NMR spectroscopy (13)C cross-polarization magic angle spinning (CPMAS) experiments were done on the 20 CC GS samples and 20 GBC GS samples of two regions. (1)H NMR spectra from the solution state NMR of all the stones reveal that cholesterol was a major component of the maximum stones of the north India region while in south Indian regions, GS had very less cholesterol. (13)C CPMAS experiments reveal that the quantity of cholesterol was significantly more in the GS of CC in the Lucknow region compared with GBC stones of Lucknow and Chandigarh. Our study also revealed that GS of the Lucknow region of both malignant and benign gallbladder diseases belong to the monohydrate crystalline form of cholesterol while GS of Chandigarh region of both malignant and benign gallbladder diseases exists in both monohydrate crystalline form with the amorphous type and anhydrous form. Gallstones have a complicated and poorly understood etiology. Therefore, it is important to understand the composition of gallstones, which can be found in various forms and clinical conditions. Variations in dietary practices, environmental conditions, and genetic factors may influence and contribute to the formation of GS. Prevention of gallstone formation may help in decreasing the cases of gallbladder cancer. |
format | Online Article Text |
id | pubmed-10289436 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-102894362023-06-24 NMR spectroscopy-based analysis of gallstones of cancerous and benign gallbladders from different geographical regions of the Indian subcontinent Siddiqui, Mohd Adnan Dwivedi, Navneet Siddiqui, Mohammed Haris Rana, S. V. Sharma, Anil Dash, N. R. Pradeep, Rebala Vijayahari, Ranjit Behari, Anu Kapoor, V. K. Sinha, Neeraj PLoS One Research Article Analysis of the chemical composition of gallstones is vital for the etiopathogenesis of gallstone diseases that can ultimately help in the prevention of its formation. In the present study, gallstones from seven different regions of India were analyzed to highlight the major difference in their composition. Also, gallstones of different pathological conditions i.e., benign (chronic cholecystitis, CC) and malignant gallbladder disease (gallbladder cancer GBC) were characterized. The type of polymorphs of cholesterol molecules was also studied to provide insight into the structure of gallstones. (1)H solution state NMR spectroscopy 1D experiments were performed on a total of 94 gallstone (GS) samples collected from seven different geographical regions of India. Solid-State NMR spectroscopy (13)C cross-polarization magic angle spinning (CPMAS) experiments were done on the 20 CC GS samples and 20 GBC GS samples of two regions. (1)H NMR spectra from the solution state NMR of all the stones reveal that cholesterol was a major component of the maximum stones of the north India region while in south Indian regions, GS had very less cholesterol. (13)C CPMAS experiments reveal that the quantity of cholesterol was significantly more in the GS of CC in the Lucknow region compared with GBC stones of Lucknow and Chandigarh. Our study also revealed that GS of the Lucknow region of both malignant and benign gallbladder diseases belong to the monohydrate crystalline form of cholesterol while GS of Chandigarh region of both malignant and benign gallbladder diseases exists in both monohydrate crystalline form with the amorphous type and anhydrous form. Gallstones have a complicated and poorly understood etiology. Therefore, it is important to understand the composition of gallstones, which can be found in various forms and clinical conditions. Variations in dietary practices, environmental conditions, and genetic factors may influence and contribute to the formation of GS. Prevention of gallstone formation may help in decreasing the cases of gallbladder cancer. Public Library of Science 2023-06-23 /pmc/articles/PMC10289436/ /pubmed/37352214 http://dx.doi.org/10.1371/journal.pone.0286979 Text en © 2023 Siddiqui et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Siddiqui, Mohd Adnan Dwivedi, Navneet Siddiqui, Mohammed Haris Rana, S. V. Sharma, Anil Dash, N. R. Pradeep, Rebala Vijayahari, Ranjit Behari, Anu Kapoor, V. K. Sinha, Neeraj NMR spectroscopy-based analysis of gallstones of cancerous and benign gallbladders from different geographical regions of the Indian subcontinent |
title | NMR spectroscopy-based analysis of gallstones of cancerous and benign gallbladders from different geographical regions of the Indian subcontinent |
title_full | NMR spectroscopy-based analysis of gallstones of cancerous and benign gallbladders from different geographical regions of the Indian subcontinent |
title_fullStr | NMR spectroscopy-based analysis of gallstones of cancerous and benign gallbladders from different geographical regions of the Indian subcontinent |
title_full_unstemmed | NMR spectroscopy-based analysis of gallstones of cancerous and benign gallbladders from different geographical regions of the Indian subcontinent |
title_short | NMR spectroscopy-based analysis of gallstones of cancerous and benign gallbladders from different geographical regions of the Indian subcontinent |
title_sort | nmr spectroscopy-based analysis of gallstones of cancerous and benign gallbladders from different geographical regions of the indian subcontinent |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10289436/ https://www.ncbi.nlm.nih.gov/pubmed/37352214 http://dx.doi.org/10.1371/journal.pone.0286979 |
work_keys_str_mv | AT siddiquimohdadnan nmrspectroscopybasedanalysisofgallstonesofcancerousandbenigngallbladdersfromdifferentgeographicalregionsoftheindiansubcontinent AT dwivedinavneet nmrspectroscopybasedanalysisofgallstonesofcancerousandbenigngallbladdersfromdifferentgeographicalregionsoftheindiansubcontinent AT siddiquimohammedharis nmrspectroscopybasedanalysisofgallstonesofcancerousandbenigngallbladdersfromdifferentgeographicalregionsoftheindiansubcontinent AT ranasv nmrspectroscopybasedanalysisofgallstonesofcancerousandbenigngallbladdersfromdifferentgeographicalregionsoftheindiansubcontinent AT sharmaanil nmrspectroscopybasedanalysisofgallstonesofcancerousandbenigngallbladdersfromdifferentgeographicalregionsoftheindiansubcontinent AT dashnr nmrspectroscopybasedanalysisofgallstonesofcancerousandbenigngallbladdersfromdifferentgeographicalregionsoftheindiansubcontinent AT pradeeprebala nmrspectroscopybasedanalysisofgallstonesofcancerousandbenigngallbladdersfromdifferentgeographicalregionsoftheindiansubcontinent AT vijayahariranjit nmrspectroscopybasedanalysisofgallstonesofcancerousandbenigngallbladdersfromdifferentgeographicalregionsoftheindiansubcontinent AT beharianu nmrspectroscopybasedanalysisofgallstonesofcancerousandbenigngallbladdersfromdifferentgeographicalregionsoftheindiansubcontinent AT kapoorvk nmrspectroscopybasedanalysisofgallstonesofcancerousandbenigngallbladdersfromdifferentgeographicalregionsoftheindiansubcontinent AT sinhaneeraj nmrspectroscopybasedanalysisofgallstonesofcancerousandbenigngallbladdersfromdifferentgeographicalregionsoftheindiansubcontinent |