Cargando…

NMR spectroscopy-based analysis of gallstones of cancerous and benign gallbladders from different geographical regions of the Indian subcontinent

Analysis of the chemical composition of gallstones is vital for the etiopathogenesis of gallstone diseases that can ultimately help in the prevention of its formation. In the present study, gallstones from seven different regions of India were analyzed to highlight the major difference in their comp...

Descripción completa

Detalles Bibliográficos
Autores principales: Siddiqui, Mohd Adnan, Dwivedi, Navneet, Siddiqui, Mohammed Haris, Rana, S. V., Sharma, Anil, Dash, N. R., Pradeep, Rebala, Vijayahari, Ranjit, Behari, Anu, Kapoor, V. K., Sinha, Neeraj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10289436/
https://www.ncbi.nlm.nih.gov/pubmed/37352214
http://dx.doi.org/10.1371/journal.pone.0286979
_version_ 1785062278035406848
author Siddiqui, Mohd Adnan
Dwivedi, Navneet
Siddiqui, Mohammed Haris
Rana, S. V.
Sharma, Anil
Dash, N. R.
Pradeep, Rebala
Vijayahari, Ranjit
Behari, Anu
Kapoor, V. K.
Sinha, Neeraj
author_facet Siddiqui, Mohd Adnan
Dwivedi, Navneet
Siddiqui, Mohammed Haris
Rana, S. V.
Sharma, Anil
Dash, N. R.
Pradeep, Rebala
Vijayahari, Ranjit
Behari, Anu
Kapoor, V. K.
Sinha, Neeraj
author_sort Siddiqui, Mohd Adnan
collection PubMed
description Analysis of the chemical composition of gallstones is vital for the etiopathogenesis of gallstone diseases that can ultimately help in the prevention of its formation. In the present study, gallstones from seven different regions of India were analyzed to highlight the major difference in their composition. Also, gallstones of different pathological conditions i.e., benign (chronic cholecystitis, CC) and malignant gallbladder disease (gallbladder cancer GBC) were characterized. The type of polymorphs of cholesterol molecules was also studied to provide insight into the structure of gallstones. (1)H solution state NMR spectroscopy 1D experiments were performed on a total of 94 gallstone (GS) samples collected from seven different geographical regions of India. Solid-State NMR spectroscopy (13)C cross-polarization magic angle spinning (CPMAS) experiments were done on the 20 CC GS samples and 20 GBC GS samples of two regions. (1)H NMR spectra from the solution state NMR of all the stones reveal that cholesterol was a major component of the maximum stones of the north India region while in south Indian regions, GS had very less cholesterol. (13)C CPMAS experiments reveal that the quantity of cholesterol was significantly more in the GS of CC in the Lucknow region compared with GBC stones of Lucknow and Chandigarh. Our study also revealed that GS of the Lucknow region of both malignant and benign gallbladder diseases belong to the monohydrate crystalline form of cholesterol while GS of Chandigarh region of both malignant and benign gallbladder diseases exists in both monohydrate crystalline form with the amorphous type and anhydrous form. Gallstones have a complicated and poorly understood etiology. Therefore, it is important to understand the composition of gallstones, which can be found in various forms and clinical conditions. Variations in dietary practices, environmental conditions, and genetic factors may influence and contribute to the formation of GS. Prevention of gallstone formation may help in decreasing the cases of gallbladder cancer.
format Online
Article
Text
id pubmed-10289436
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-102894362023-06-24 NMR spectroscopy-based analysis of gallstones of cancerous and benign gallbladders from different geographical regions of the Indian subcontinent Siddiqui, Mohd Adnan Dwivedi, Navneet Siddiqui, Mohammed Haris Rana, S. V. Sharma, Anil Dash, N. R. Pradeep, Rebala Vijayahari, Ranjit Behari, Anu Kapoor, V. K. Sinha, Neeraj PLoS One Research Article Analysis of the chemical composition of gallstones is vital for the etiopathogenesis of gallstone diseases that can ultimately help in the prevention of its formation. In the present study, gallstones from seven different regions of India were analyzed to highlight the major difference in their composition. Also, gallstones of different pathological conditions i.e., benign (chronic cholecystitis, CC) and malignant gallbladder disease (gallbladder cancer GBC) were characterized. The type of polymorphs of cholesterol molecules was also studied to provide insight into the structure of gallstones. (1)H solution state NMR spectroscopy 1D experiments were performed on a total of 94 gallstone (GS) samples collected from seven different geographical regions of India. Solid-State NMR spectroscopy (13)C cross-polarization magic angle spinning (CPMAS) experiments were done on the 20 CC GS samples and 20 GBC GS samples of two regions. (1)H NMR spectra from the solution state NMR of all the stones reveal that cholesterol was a major component of the maximum stones of the north India region while in south Indian regions, GS had very less cholesterol. (13)C CPMAS experiments reveal that the quantity of cholesterol was significantly more in the GS of CC in the Lucknow region compared with GBC stones of Lucknow and Chandigarh. Our study also revealed that GS of the Lucknow region of both malignant and benign gallbladder diseases belong to the monohydrate crystalline form of cholesterol while GS of Chandigarh region of both malignant and benign gallbladder diseases exists in both monohydrate crystalline form with the amorphous type and anhydrous form. Gallstones have a complicated and poorly understood etiology. Therefore, it is important to understand the composition of gallstones, which can be found in various forms and clinical conditions. Variations in dietary practices, environmental conditions, and genetic factors may influence and contribute to the formation of GS. Prevention of gallstone formation may help in decreasing the cases of gallbladder cancer. Public Library of Science 2023-06-23 /pmc/articles/PMC10289436/ /pubmed/37352214 http://dx.doi.org/10.1371/journal.pone.0286979 Text en © 2023 Siddiqui et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Siddiqui, Mohd Adnan
Dwivedi, Navneet
Siddiqui, Mohammed Haris
Rana, S. V.
Sharma, Anil
Dash, N. R.
Pradeep, Rebala
Vijayahari, Ranjit
Behari, Anu
Kapoor, V. K.
Sinha, Neeraj
NMR spectroscopy-based analysis of gallstones of cancerous and benign gallbladders from different geographical regions of the Indian subcontinent
title NMR spectroscopy-based analysis of gallstones of cancerous and benign gallbladders from different geographical regions of the Indian subcontinent
title_full NMR spectroscopy-based analysis of gallstones of cancerous and benign gallbladders from different geographical regions of the Indian subcontinent
title_fullStr NMR spectroscopy-based analysis of gallstones of cancerous and benign gallbladders from different geographical regions of the Indian subcontinent
title_full_unstemmed NMR spectroscopy-based analysis of gallstones of cancerous and benign gallbladders from different geographical regions of the Indian subcontinent
title_short NMR spectroscopy-based analysis of gallstones of cancerous and benign gallbladders from different geographical regions of the Indian subcontinent
title_sort nmr spectroscopy-based analysis of gallstones of cancerous and benign gallbladders from different geographical regions of the indian subcontinent
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10289436/
https://www.ncbi.nlm.nih.gov/pubmed/37352214
http://dx.doi.org/10.1371/journal.pone.0286979
work_keys_str_mv AT siddiquimohdadnan nmrspectroscopybasedanalysisofgallstonesofcancerousandbenigngallbladdersfromdifferentgeographicalregionsoftheindiansubcontinent
AT dwivedinavneet nmrspectroscopybasedanalysisofgallstonesofcancerousandbenigngallbladdersfromdifferentgeographicalregionsoftheindiansubcontinent
AT siddiquimohammedharis nmrspectroscopybasedanalysisofgallstonesofcancerousandbenigngallbladdersfromdifferentgeographicalregionsoftheindiansubcontinent
AT ranasv nmrspectroscopybasedanalysisofgallstonesofcancerousandbenigngallbladdersfromdifferentgeographicalregionsoftheindiansubcontinent
AT sharmaanil nmrspectroscopybasedanalysisofgallstonesofcancerousandbenigngallbladdersfromdifferentgeographicalregionsoftheindiansubcontinent
AT dashnr nmrspectroscopybasedanalysisofgallstonesofcancerousandbenigngallbladdersfromdifferentgeographicalregionsoftheindiansubcontinent
AT pradeeprebala nmrspectroscopybasedanalysisofgallstonesofcancerousandbenigngallbladdersfromdifferentgeographicalregionsoftheindiansubcontinent
AT vijayahariranjit nmrspectroscopybasedanalysisofgallstonesofcancerousandbenigngallbladdersfromdifferentgeographicalregionsoftheindiansubcontinent
AT beharianu nmrspectroscopybasedanalysisofgallstonesofcancerousandbenigngallbladdersfromdifferentgeographicalregionsoftheindiansubcontinent
AT kapoorvk nmrspectroscopybasedanalysisofgallstonesofcancerousandbenigngallbladdersfromdifferentgeographicalregionsoftheindiansubcontinent
AT sinhaneeraj nmrspectroscopybasedanalysisofgallstonesofcancerousandbenigngallbladdersfromdifferentgeographicalregionsoftheindiansubcontinent