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Mass spectrometry imaging of hair identifies daily maraviroc adherence in HPTN 069/ACTG A5305
Objective measures of adherence for antiretrovirals used as pre-exposure prophylaxis (PrEP) are critical for improving preventative efficacy in both clinical trials and real-world application. Current objective adherence measures either reflect only recent behavior (eg days for plasma or urine) or c...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10289441/ https://www.ncbi.nlm.nih.gov/pubmed/37352285 http://dx.doi.org/10.1371/journal.pone.0287449 |
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author | Rosen, Elias P. White, Nicole Gilliland, William M. Gerona, Roy R. Gandhi, Monica Amico, K. Rivet Mayer, Kenneth H. Gulick, Roy M. Kashuba, Angela D. M. |
author_facet | Rosen, Elias P. White, Nicole Gilliland, William M. Gerona, Roy R. Gandhi, Monica Amico, K. Rivet Mayer, Kenneth H. Gulick, Roy M. Kashuba, Angela D. M. |
author_sort | Rosen, Elias P. |
collection | PubMed |
description | Objective measures of adherence for antiretrovirals used as pre-exposure prophylaxis (PrEP) are critical for improving preventative efficacy in both clinical trials and real-world application. Current objective adherence measures either reflect only recent behavior (eg days for plasma or urine) or cumulative behavior (eg months for dried blood spots). Here, we measured the accumulation of the antiretroviral drug maraviroc (MVC) in hair strands by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging (MSI) to evaluate adherence behavior longitudinally at high temporal resolution. An MSI threshold for classifying daily adherence was established using clinical samples from healthy volunteers following directly observed dosing of 1 to 7 doses MVC/week. We then used the benchmarked MSI assay to classify adherence to MVC-based PrEP regimens in hair samples collected throughout the 48-week HPTN069/ACTGA5305 study. We found that only ~32% of investigated hair samples collected during the study’s active dosing period showed consistent daily PrEP adherence throughout a retrospective period of 30 days, and also found that profiles of daily individual adherence from MSI hair analysis could identify when patients were and were not taking study drug. The assessment of adherence from MSI hair strand analysis was 62% lower than adherence classified using paired plasma samples, the latter of which may be influenced by white-coat adherence. These findings demonstrate the ability of MSI hair analysis to examine daily variability of adherence behavior over a longer-term measurement and offer the potential for longitudinal comparison with risk behavior to target patient-specific adherence interventions and improve outcomes. |
format | Online Article Text |
id | pubmed-10289441 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-102894412023-06-24 Mass spectrometry imaging of hair identifies daily maraviroc adherence in HPTN 069/ACTG A5305 Rosen, Elias P. White, Nicole Gilliland, William M. Gerona, Roy R. Gandhi, Monica Amico, K. Rivet Mayer, Kenneth H. Gulick, Roy M. Kashuba, Angela D. M. PLoS One Research Article Objective measures of adherence for antiretrovirals used as pre-exposure prophylaxis (PrEP) are critical for improving preventative efficacy in both clinical trials and real-world application. Current objective adherence measures either reflect only recent behavior (eg days for plasma or urine) or cumulative behavior (eg months for dried blood spots). Here, we measured the accumulation of the antiretroviral drug maraviroc (MVC) in hair strands by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging (MSI) to evaluate adherence behavior longitudinally at high temporal resolution. An MSI threshold for classifying daily adherence was established using clinical samples from healthy volunteers following directly observed dosing of 1 to 7 doses MVC/week. We then used the benchmarked MSI assay to classify adherence to MVC-based PrEP regimens in hair samples collected throughout the 48-week HPTN069/ACTGA5305 study. We found that only ~32% of investigated hair samples collected during the study’s active dosing period showed consistent daily PrEP adherence throughout a retrospective period of 30 days, and also found that profiles of daily individual adherence from MSI hair analysis could identify when patients were and were not taking study drug. The assessment of adherence from MSI hair strand analysis was 62% lower than adherence classified using paired plasma samples, the latter of which may be influenced by white-coat adherence. These findings demonstrate the ability of MSI hair analysis to examine daily variability of adherence behavior over a longer-term measurement and offer the potential for longitudinal comparison with risk behavior to target patient-specific adherence interventions and improve outcomes. Public Library of Science 2023-06-23 /pmc/articles/PMC10289441/ /pubmed/37352285 http://dx.doi.org/10.1371/journal.pone.0287449 Text en © 2023 Rosen et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Rosen, Elias P. White, Nicole Gilliland, William M. Gerona, Roy R. Gandhi, Monica Amico, K. Rivet Mayer, Kenneth H. Gulick, Roy M. Kashuba, Angela D. M. Mass spectrometry imaging of hair identifies daily maraviroc adherence in HPTN 069/ACTG A5305 |
title | Mass spectrometry imaging of hair identifies daily maraviroc adherence in HPTN 069/ACTG A5305 |
title_full | Mass spectrometry imaging of hair identifies daily maraviroc adherence in HPTN 069/ACTG A5305 |
title_fullStr | Mass spectrometry imaging of hair identifies daily maraviroc adherence in HPTN 069/ACTG A5305 |
title_full_unstemmed | Mass spectrometry imaging of hair identifies daily maraviroc adherence in HPTN 069/ACTG A5305 |
title_short | Mass spectrometry imaging of hair identifies daily maraviroc adherence in HPTN 069/ACTG A5305 |
title_sort | mass spectrometry imaging of hair identifies daily maraviroc adherence in hptn 069/actg a5305 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10289441/ https://www.ncbi.nlm.nih.gov/pubmed/37352285 http://dx.doi.org/10.1371/journal.pone.0287449 |
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