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Neuroprotective effect of Astragali Radix on cerebral infarction based on proteomics

Objective: Astragali Radix (AR, Huangqi in Chinese) has a neuroprotective effect on cerebral infarction (CI). In order to explore the biological basis and therapeutic mechanism of AR in CI, a double-blind randomized controlled trial was established in this study, and proteomics analysis was carried...

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Autores principales: Li, Ying, Wang, Daoping, Guo, Rongjuan, Ma, Bo, Miao, Lan, Sun, Mingqian, He, Lijuan, Lin, Li, Pan, Yinghong, Ren, Junguo, Liu, Jianxun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10289882/
https://www.ncbi.nlm.nih.gov/pubmed/37361203
http://dx.doi.org/10.3389/fphar.2023.1162134
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author Li, Ying
Wang, Daoping
Guo, Rongjuan
Ma, Bo
Miao, Lan
Sun, Mingqian
He, Lijuan
Lin, Li
Pan, Yinghong
Ren, Junguo
Liu, Jianxun
author_facet Li, Ying
Wang, Daoping
Guo, Rongjuan
Ma, Bo
Miao, Lan
Sun, Mingqian
He, Lijuan
Lin, Li
Pan, Yinghong
Ren, Junguo
Liu, Jianxun
author_sort Li, Ying
collection PubMed
description Objective: Astragali Radix (AR, Huangqi in Chinese) has a neuroprotective effect on cerebral infarction (CI). In order to explore the biological basis and therapeutic mechanism of AR in CI, a double-blind randomized controlled trial was established in this study, and proteomics analysis was carried out on serum samples of patients. Methods: The patients were divided into the AR group (n = 35) and the control group (n = 30). The curative effect was evaluated by the traditional Chinese medicine (TCM) syndrome score and clinical indicators, and the serum of the two groups was analyzed by proteomics. Based on bioinformatics analysis methods, the changes in differential proteins between two groups of samples were explored, and the key proteins were validated through enzyme-linked immunosorbent assay (ELISA). Results: The results of this study showed that the scores of deficiency of vital energy (DVE), blood stasis (BS), and NIH Stroke Scale (NIHSS) decreased significantly (p < 0.05), while the scores of the Barthel Index (BI) increased, indicating that AR could significantly improve the symptoms of CI patients. In addition, we found that compared with the control group, AR upregulated 43 proteins and downregulated 20 proteins, especially focusing on anti-atherosclerosis and neuroprotective effects. Moreover, ELISA indicated the levels of IL-6, TNF-α, VCAM-1, MCP-1, and ICAM-1 were significantly decreased in the serum of the AR group (p < 0.05, p < 0.01). Conclusion: This study found that AR can significantly recover the clinical symptoms of CI. Serum proteomics research results show that AR may act on IL-6, TNF-α, VCAM-1, MCP-1, and ICAM-1, and play anti-atherosclerosis and neuroprotective roles. Clinical Trial Registration: [clinicaltrials.gov], identifier [NCT02846207]
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spelling pubmed-102898822023-06-24 Neuroprotective effect of Astragali Radix on cerebral infarction based on proteomics Li, Ying Wang, Daoping Guo, Rongjuan Ma, Bo Miao, Lan Sun, Mingqian He, Lijuan Lin, Li Pan, Yinghong Ren, Junguo Liu, Jianxun Front Pharmacol Pharmacology Objective: Astragali Radix (AR, Huangqi in Chinese) has a neuroprotective effect on cerebral infarction (CI). In order to explore the biological basis and therapeutic mechanism of AR in CI, a double-blind randomized controlled trial was established in this study, and proteomics analysis was carried out on serum samples of patients. Methods: The patients were divided into the AR group (n = 35) and the control group (n = 30). The curative effect was evaluated by the traditional Chinese medicine (TCM) syndrome score and clinical indicators, and the serum of the two groups was analyzed by proteomics. Based on bioinformatics analysis methods, the changes in differential proteins between two groups of samples were explored, and the key proteins were validated through enzyme-linked immunosorbent assay (ELISA). Results: The results of this study showed that the scores of deficiency of vital energy (DVE), blood stasis (BS), and NIH Stroke Scale (NIHSS) decreased significantly (p < 0.05), while the scores of the Barthel Index (BI) increased, indicating that AR could significantly improve the symptoms of CI patients. In addition, we found that compared with the control group, AR upregulated 43 proteins and downregulated 20 proteins, especially focusing on anti-atherosclerosis and neuroprotective effects. Moreover, ELISA indicated the levels of IL-6, TNF-α, VCAM-1, MCP-1, and ICAM-1 were significantly decreased in the serum of the AR group (p < 0.05, p < 0.01). Conclusion: This study found that AR can significantly recover the clinical symptoms of CI. Serum proteomics research results show that AR may act on IL-6, TNF-α, VCAM-1, MCP-1, and ICAM-1, and play anti-atherosclerosis and neuroprotective roles. Clinical Trial Registration: [clinicaltrials.gov], identifier [NCT02846207] Frontiers Media S.A. 2023-06-09 /pmc/articles/PMC10289882/ /pubmed/37361203 http://dx.doi.org/10.3389/fphar.2023.1162134 Text en Copyright © 2023 Li, Wang, Guo, Ma, Miao, Sun, He, Lin, Pan, Ren and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Li, Ying
Wang, Daoping
Guo, Rongjuan
Ma, Bo
Miao, Lan
Sun, Mingqian
He, Lijuan
Lin, Li
Pan, Yinghong
Ren, Junguo
Liu, Jianxun
Neuroprotective effect of Astragali Radix on cerebral infarction based on proteomics
title Neuroprotective effect of Astragali Radix on cerebral infarction based on proteomics
title_full Neuroprotective effect of Astragali Radix on cerebral infarction based on proteomics
title_fullStr Neuroprotective effect of Astragali Radix on cerebral infarction based on proteomics
title_full_unstemmed Neuroprotective effect of Astragali Radix on cerebral infarction based on proteomics
title_short Neuroprotective effect of Astragali Radix on cerebral infarction based on proteomics
title_sort neuroprotective effect of astragali radix on cerebral infarction based on proteomics
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10289882/
https://www.ncbi.nlm.nih.gov/pubmed/37361203
http://dx.doi.org/10.3389/fphar.2023.1162134
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