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Neuroprotective effect of Astragali Radix on cerebral infarction based on proteomics
Objective: Astragali Radix (AR, Huangqi in Chinese) has a neuroprotective effect on cerebral infarction (CI). In order to explore the biological basis and therapeutic mechanism of AR in CI, a double-blind randomized controlled trial was established in this study, and proteomics analysis was carried...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10289882/ https://www.ncbi.nlm.nih.gov/pubmed/37361203 http://dx.doi.org/10.3389/fphar.2023.1162134 |
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author | Li, Ying Wang, Daoping Guo, Rongjuan Ma, Bo Miao, Lan Sun, Mingqian He, Lijuan Lin, Li Pan, Yinghong Ren, Junguo Liu, Jianxun |
author_facet | Li, Ying Wang, Daoping Guo, Rongjuan Ma, Bo Miao, Lan Sun, Mingqian He, Lijuan Lin, Li Pan, Yinghong Ren, Junguo Liu, Jianxun |
author_sort | Li, Ying |
collection | PubMed |
description | Objective: Astragali Radix (AR, Huangqi in Chinese) has a neuroprotective effect on cerebral infarction (CI). In order to explore the biological basis and therapeutic mechanism of AR in CI, a double-blind randomized controlled trial was established in this study, and proteomics analysis was carried out on serum samples of patients. Methods: The patients were divided into the AR group (n = 35) and the control group (n = 30). The curative effect was evaluated by the traditional Chinese medicine (TCM) syndrome score and clinical indicators, and the serum of the two groups was analyzed by proteomics. Based on bioinformatics analysis methods, the changes in differential proteins between two groups of samples were explored, and the key proteins were validated through enzyme-linked immunosorbent assay (ELISA). Results: The results of this study showed that the scores of deficiency of vital energy (DVE), blood stasis (BS), and NIH Stroke Scale (NIHSS) decreased significantly (p < 0.05), while the scores of the Barthel Index (BI) increased, indicating that AR could significantly improve the symptoms of CI patients. In addition, we found that compared with the control group, AR upregulated 43 proteins and downregulated 20 proteins, especially focusing on anti-atherosclerosis and neuroprotective effects. Moreover, ELISA indicated the levels of IL-6, TNF-α, VCAM-1, MCP-1, and ICAM-1 were significantly decreased in the serum of the AR group (p < 0.05, p < 0.01). Conclusion: This study found that AR can significantly recover the clinical symptoms of CI. Serum proteomics research results show that AR may act on IL-6, TNF-α, VCAM-1, MCP-1, and ICAM-1, and play anti-atherosclerosis and neuroprotective roles. Clinical Trial Registration: [clinicaltrials.gov], identifier [NCT02846207] |
format | Online Article Text |
id | pubmed-10289882 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102898822023-06-24 Neuroprotective effect of Astragali Radix on cerebral infarction based on proteomics Li, Ying Wang, Daoping Guo, Rongjuan Ma, Bo Miao, Lan Sun, Mingqian He, Lijuan Lin, Li Pan, Yinghong Ren, Junguo Liu, Jianxun Front Pharmacol Pharmacology Objective: Astragali Radix (AR, Huangqi in Chinese) has a neuroprotective effect on cerebral infarction (CI). In order to explore the biological basis and therapeutic mechanism of AR in CI, a double-blind randomized controlled trial was established in this study, and proteomics analysis was carried out on serum samples of patients. Methods: The patients were divided into the AR group (n = 35) and the control group (n = 30). The curative effect was evaluated by the traditional Chinese medicine (TCM) syndrome score and clinical indicators, and the serum of the two groups was analyzed by proteomics. Based on bioinformatics analysis methods, the changes in differential proteins between two groups of samples were explored, and the key proteins were validated through enzyme-linked immunosorbent assay (ELISA). Results: The results of this study showed that the scores of deficiency of vital energy (DVE), blood stasis (BS), and NIH Stroke Scale (NIHSS) decreased significantly (p < 0.05), while the scores of the Barthel Index (BI) increased, indicating that AR could significantly improve the symptoms of CI patients. In addition, we found that compared with the control group, AR upregulated 43 proteins and downregulated 20 proteins, especially focusing on anti-atherosclerosis and neuroprotective effects. Moreover, ELISA indicated the levels of IL-6, TNF-α, VCAM-1, MCP-1, and ICAM-1 were significantly decreased in the serum of the AR group (p < 0.05, p < 0.01). Conclusion: This study found that AR can significantly recover the clinical symptoms of CI. Serum proteomics research results show that AR may act on IL-6, TNF-α, VCAM-1, MCP-1, and ICAM-1, and play anti-atherosclerosis and neuroprotective roles. Clinical Trial Registration: [clinicaltrials.gov], identifier [NCT02846207] Frontiers Media S.A. 2023-06-09 /pmc/articles/PMC10289882/ /pubmed/37361203 http://dx.doi.org/10.3389/fphar.2023.1162134 Text en Copyright © 2023 Li, Wang, Guo, Ma, Miao, Sun, He, Lin, Pan, Ren and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Li, Ying Wang, Daoping Guo, Rongjuan Ma, Bo Miao, Lan Sun, Mingqian He, Lijuan Lin, Li Pan, Yinghong Ren, Junguo Liu, Jianxun Neuroprotective effect of Astragali Radix on cerebral infarction based on proteomics |
title | Neuroprotective effect of Astragali Radix on cerebral infarction based on proteomics |
title_full | Neuroprotective effect of Astragali Radix on cerebral infarction based on proteomics |
title_fullStr | Neuroprotective effect of Astragali Radix on cerebral infarction based on proteomics |
title_full_unstemmed | Neuroprotective effect of Astragali Radix on cerebral infarction based on proteomics |
title_short | Neuroprotective effect of Astragali Radix on cerebral infarction based on proteomics |
title_sort | neuroprotective effect of astragali radix on cerebral infarction based on proteomics |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10289882/ https://www.ncbi.nlm.nih.gov/pubmed/37361203 http://dx.doi.org/10.3389/fphar.2023.1162134 |
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