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Pre-sleep Protein Ingestion Increases Mitochondrial Protein Synthesis Rates During Overnight Recovery from Endurance Exercise: A Randomized Controlled Trial
BACKGROUND: Casein protein ingestion prior to sleep has been shown to increase myofibrillar protein synthesis rates during overnight sleep. It remains to be assessed whether pre-sleep protein ingestion can also increase mitochondrial protein synthesis rates. Though it has been suggested that casein...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10289916/ https://www.ncbi.nlm.nih.gov/pubmed/36857005 http://dx.doi.org/10.1007/s40279-023-01822-3 |
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author | Trommelen, Jorn van Lieshout, Glenn A. A. Pabla, Pardeep Nyakayiru, Jean Hendriks, Floris K. Senden, Joan M. Goessens, Joy P. B. van Kranenburg, Janneau M. X. Gijsen, Annemie P. Verdijk, Lex B. de Groot, Lisette C. P. G. M. van Loon, Luc J. C. |
author_facet | Trommelen, Jorn van Lieshout, Glenn A. A. Pabla, Pardeep Nyakayiru, Jean Hendriks, Floris K. Senden, Joan M. Goessens, Joy P. B. van Kranenburg, Janneau M. X. Gijsen, Annemie P. Verdijk, Lex B. de Groot, Lisette C. P. G. M. van Loon, Luc J. C. |
author_sort | Trommelen, Jorn |
collection | PubMed |
description | BACKGROUND: Casein protein ingestion prior to sleep has been shown to increase myofibrillar protein synthesis rates during overnight sleep. It remains to be assessed whether pre-sleep protein ingestion can also increase mitochondrial protein synthesis rates. Though it has been suggested that casein protein may be preferred as a pre-sleep protein source, no study has compared the impact of pre-sleep whey versus casein ingestion on overnight muscle protein synthesis rates. OBJECTIVE: We aimed to assess the impact of casein and whey protein ingestion prior to sleep on mitochondrial and myofibrillar protein synthesis rates during overnight recovery from a bout of endurance-type exercise. METHODS: Thirty-six healthy young men performed a single bout of endurance-type exercise in the evening (19:45 h). Thirty minutes prior to sleep (23:30 h), participants ingested 45 g of casein protein, 45 g of whey protein, or a non-caloric placebo. Continuous intravenous l-[ring-(13)C(6)]-phenylalanine infusions were applied, with blood and muscle tissue samples being collected to assess overnight mitochondrial and myofibrillar protein synthesis rates. RESULTS: Pooled protein ingestion resulted in greater mitochondrial (0.087 ± 0.020 vs 0.067 ± 0.016%·h(−1), p = 0.005) and myofibrillar (0.060 ± 0.014 vs 0.047 ± 0.011%·h(−1), p = 0.012) protein synthesis rates when compared with placebo. Casein and whey protein ingestion did not differ in their capacity to stimulate mitochondrial (0.082 ± 0.019 vs 0.092 ± 0.020%·h(−1), p = 0.690) and myofibrillar (0.056 ± 0.009 vs 0.064 ± 0.018%·h(−1), p = 0.440) protein synthesis rates. CONCLUSIONS: Protein ingestion prior to sleep increases both mitochondrial and myofibrillar protein synthesis rates during overnight recovery from exercise. The overnight muscle protein synthetic response to whey and casein protein does not differ. CLINICAL TRIAL REGISTRATION: NTR7251. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s40279-023-01822-3. |
format | Online Article Text |
id | pubmed-10289916 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-102899162023-06-25 Pre-sleep Protein Ingestion Increases Mitochondrial Protein Synthesis Rates During Overnight Recovery from Endurance Exercise: A Randomized Controlled Trial Trommelen, Jorn van Lieshout, Glenn A. A. Pabla, Pardeep Nyakayiru, Jean Hendriks, Floris K. Senden, Joan M. Goessens, Joy P. B. van Kranenburg, Janneau M. X. Gijsen, Annemie P. Verdijk, Lex B. de Groot, Lisette C. P. G. M. van Loon, Luc J. C. Sports Med Original Research Article BACKGROUND: Casein protein ingestion prior to sleep has been shown to increase myofibrillar protein synthesis rates during overnight sleep. It remains to be assessed whether pre-sleep protein ingestion can also increase mitochondrial protein synthesis rates. Though it has been suggested that casein protein may be preferred as a pre-sleep protein source, no study has compared the impact of pre-sleep whey versus casein ingestion on overnight muscle protein synthesis rates. OBJECTIVE: We aimed to assess the impact of casein and whey protein ingestion prior to sleep on mitochondrial and myofibrillar protein synthesis rates during overnight recovery from a bout of endurance-type exercise. METHODS: Thirty-six healthy young men performed a single bout of endurance-type exercise in the evening (19:45 h). Thirty minutes prior to sleep (23:30 h), participants ingested 45 g of casein protein, 45 g of whey protein, or a non-caloric placebo. Continuous intravenous l-[ring-(13)C(6)]-phenylalanine infusions were applied, with blood and muscle tissue samples being collected to assess overnight mitochondrial and myofibrillar protein synthesis rates. RESULTS: Pooled protein ingestion resulted in greater mitochondrial (0.087 ± 0.020 vs 0.067 ± 0.016%·h(−1), p = 0.005) and myofibrillar (0.060 ± 0.014 vs 0.047 ± 0.011%·h(−1), p = 0.012) protein synthesis rates when compared with placebo. Casein and whey protein ingestion did not differ in their capacity to stimulate mitochondrial (0.082 ± 0.019 vs 0.092 ± 0.020%·h(−1), p = 0.690) and myofibrillar (0.056 ± 0.009 vs 0.064 ± 0.018%·h(−1), p = 0.440) protein synthesis rates. CONCLUSIONS: Protein ingestion prior to sleep increases both mitochondrial and myofibrillar protein synthesis rates during overnight recovery from exercise. The overnight muscle protein synthetic response to whey and casein protein does not differ. CLINICAL TRIAL REGISTRATION: NTR7251. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s40279-023-01822-3. Springer International Publishing 2023-03-01 2023 /pmc/articles/PMC10289916/ /pubmed/36857005 http://dx.doi.org/10.1007/s40279-023-01822-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Research Article Trommelen, Jorn van Lieshout, Glenn A. A. Pabla, Pardeep Nyakayiru, Jean Hendriks, Floris K. Senden, Joan M. Goessens, Joy P. B. van Kranenburg, Janneau M. X. Gijsen, Annemie P. Verdijk, Lex B. de Groot, Lisette C. P. G. M. van Loon, Luc J. C. Pre-sleep Protein Ingestion Increases Mitochondrial Protein Synthesis Rates During Overnight Recovery from Endurance Exercise: A Randomized Controlled Trial |
title | Pre-sleep Protein Ingestion Increases Mitochondrial Protein Synthesis Rates During Overnight Recovery from Endurance Exercise: A Randomized Controlled Trial |
title_full | Pre-sleep Protein Ingestion Increases Mitochondrial Protein Synthesis Rates During Overnight Recovery from Endurance Exercise: A Randomized Controlled Trial |
title_fullStr | Pre-sleep Protein Ingestion Increases Mitochondrial Protein Synthesis Rates During Overnight Recovery from Endurance Exercise: A Randomized Controlled Trial |
title_full_unstemmed | Pre-sleep Protein Ingestion Increases Mitochondrial Protein Synthesis Rates During Overnight Recovery from Endurance Exercise: A Randomized Controlled Trial |
title_short | Pre-sleep Protein Ingestion Increases Mitochondrial Protein Synthesis Rates During Overnight Recovery from Endurance Exercise: A Randomized Controlled Trial |
title_sort | pre-sleep protein ingestion increases mitochondrial protein synthesis rates during overnight recovery from endurance exercise: a randomized controlled trial |
topic | Original Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10289916/ https://www.ncbi.nlm.nih.gov/pubmed/36857005 http://dx.doi.org/10.1007/s40279-023-01822-3 |
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