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Conserved organ-specific microbial assemblages in different populations of a terrestrial crab

Microorganisms are ubiquitous in the environment and provide genetic and physiological functions to multicellular organisms. Knowledge on the associated microbiota is becoming highly relevant to understand the host’s ecology and biology. Among invertebrates, many examples of endosymbiosis have been...

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Autores principales: Bacci, Giovanni, Fratini, Sara, Meriggi, Niccolò, Cheng, Christine L. Y., Ng, Ka Hei, Pindo, Massimo, Iannucci, Alessio, Mengoni, Alessio, Cavalieri, Duccio, Cannicci, Stefano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10291174/
https://www.ncbi.nlm.nih.gov/pubmed/37378290
http://dx.doi.org/10.3389/fmicb.2023.1113617
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author Bacci, Giovanni
Fratini, Sara
Meriggi, Niccolò
Cheng, Christine L. Y.
Ng, Ka Hei
Pindo, Massimo
Iannucci, Alessio
Mengoni, Alessio
Cavalieri, Duccio
Cannicci, Stefano
author_facet Bacci, Giovanni
Fratini, Sara
Meriggi, Niccolò
Cheng, Christine L. Y.
Ng, Ka Hei
Pindo, Massimo
Iannucci, Alessio
Mengoni, Alessio
Cavalieri, Duccio
Cannicci, Stefano
author_sort Bacci, Giovanni
collection PubMed
description Microorganisms are ubiquitous in the environment and provide genetic and physiological functions to multicellular organisms. Knowledge on the associated microbiota is becoming highly relevant to understand the host’s ecology and biology. Among invertebrates, many examples of endosymbiosis have been described, such as those in corals, ants, and termites. At present, however, little is known on the presence, diversity, and putative roles of the microbiota associated to brachyuran crabs in relation to their environment. In this work we investigated the associated microbiota of three populations of the terrestrial brachyuran crab Chiromantes haematocheir to find evidence of a conserved organ-specific microbiome unrelated to the population of origin and dissimilar from environmental microbial assemblages. Bacterial 16S rRNA gene and fungal ITS sequences were obtained from selected crab organs and environmental matrices to profile microbial communities. Despite the presence of truly marine larval stages and the absence of a gregarious behaviour, favouring microbiota exchanges, we found common, organ-specific microbiota, associated with the gut and the gills of crabs from the different populations (with more than 15% of the genera detected specifically enriched only in one organ). These findings suggest the presence of possible functional roles of the organ-specific microbiota.
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spelling pubmed-102911742023-06-27 Conserved organ-specific microbial assemblages in different populations of a terrestrial crab Bacci, Giovanni Fratini, Sara Meriggi, Niccolò Cheng, Christine L. Y. Ng, Ka Hei Pindo, Massimo Iannucci, Alessio Mengoni, Alessio Cavalieri, Duccio Cannicci, Stefano Front Microbiol Microbiology Microorganisms are ubiquitous in the environment and provide genetic and physiological functions to multicellular organisms. Knowledge on the associated microbiota is becoming highly relevant to understand the host’s ecology and biology. Among invertebrates, many examples of endosymbiosis have been described, such as those in corals, ants, and termites. At present, however, little is known on the presence, diversity, and putative roles of the microbiota associated to brachyuran crabs in relation to their environment. In this work we investigated the associated microbiota of three populations of the terrestrial brachyuran crab Chiromantes haematocheir to find evidence of a conserved organ-specific microbiome unrelated to the population of origin and dissimilar from environmental microbial assemblages. Bacterial 16S rRNA gene and fungal ITS sequences were obtained from selected crab organs and environmental matrices to profile microbial communities. Despite the presence of truly marine larval stages and the absence of a gregarious behaviour, favouring microbiota exchanges, we found common, organ-specific microbiota, associated with the gut and the gills of crabs from the different populations (with more than 15% of the genera detected specifically enriched only in one organ). These findings suggest the presence of possible functional roles of the organ-specific microbiota. Frontiers Media S.A. 2023-06-12 /pmc/articles/PMC10291174/ /pubmed/37378290 http://dx.doi.org/10.3389/fmicb.2023.1113617 Text en Copyright © 2023 Bacci, Fratini, Meriggi, Cheng, Ng, Pindo, Iannucci, Mengoni, Cavalieri and Cannicci. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Bacci, Giovanni
Fratini, Sara
Meriggi, Niccolò
Cheng, Christine L. Y.
Ng, Ka Hei
Pindo, Massimo
Iannucci, Alessio
Mengoni, Alessio
Cavalieri, Duccio
Cannicci, Stefano
Conserved organ-specific microbial assemblages in different populations of a terrestrial crab
title Conserved organ-specific microbial assemblages in different populations of a terrestrial crab
title_full Conserved organ-specific microbial assemblages in different populations of a terrestrial crab
title_fullStr Conserved organ-specific microbial assemblages in different populations of a terrestrial crab
title_full_unstemmed Conserved organ-specific microbial assemblages in different populations of a terrestrial crab
title_short Conserved organ-specific microbial assemblages in different populations of a terrestrial crab
title_sort conserved organ-specific microbial assemblages in different populations of a terrestrial crab
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10291174/
https://www.ncbi.nlm.nih.gov/pubmed/37378290
http://dx.doi.org/10.3389/fmicb.2023.1113617
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